The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis
Open accessAbstract
A plasma membrane-enriched fraction prepared from barley roots was analyzed by two-dimensional gel electrophoresis. Four methods of sample solubilization were assessed on silver stained gels. When membranes were solubilized with 2% sodium dodecyl sulfate followed by addition of Nonidet P-40, gels had high background staining and few proteins because of incomplete solubilization. Gels of membranes solubilized in urea and Nonidet P-40 had a greater number of proteins but proteins with molecular weights greater than 85,000 were absent and proteins with low molecular weights were diffuse. High molecular weight proteins were present in gels of membranes solubilized in 4% sodium dodecyl sulfate followed by acetone precipitation but background staining and streaking remained a problem. Gels of the best quality were obtained when membrane proteins were extracted with phenol and precipitated with ammonium acetate in methanol; background staining and streaking were diminished and proteins were clearly resolved. This method makes possible the resolution required for meaningful qualitative and quantitative comparisons of protein patterns on two-dimensional gels of plant membrane proteins.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
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High resolution two-dimensional electrophoresis of proteins.
O'Farrell, P. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41496-8 · Cited by 19,246
Morrissey, J. H. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90783-1 · Cited by 4,061
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels
Oakley, B. R., Kirsch, D. R., & Morris, N. R. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90470-4 · Cited by 3,247
Two-dimensional gel electrophoresis of membrane proteins
Ames, G. F. L., & Nikaido, K. (1976) · Biochemistry · doi:10.1021/bi00648a026 · Cited by 697
Uemura, M., & Yoshida, S. (1984) · PLANT PHYSIOLOGY · doi:10.1104/pp.75.3.818 · Cited by 137
Zurfluh, L. L., & Guilfoyle, T. J. (1982) · PLANT PHYSIOLOGY · doi:10.1104/pp.69.2.332 · Cited by 115
Cited by
Pontier, D., Yahubyan, G., Vega, D., Bulski, A., Saez-Vasquez, J., Hakimi, M.-A., Lerbs-Mache, S., Colot, V., & Lagrange, T. (2005) · Genes & Development · doi:10.1101/gad.348405 · Cited by 382
Wang, H., Alvarez, S., & Hicks, L. M. (2012) · Journal of Proteome Research · doi:10.1021/pr2008225 · Cited by 142
Trejo-Hernández, A., Andrade-Domínguez, A., Hernández, M., & Encarnación, S. (2014) · The ISME Journal · doi:10.1038/ismej.2014.53 · Cited by 135
Tétard-Jones, C., Edwards, M., Rempelos, L., Gatehouse, A., Eyre, M., Wilcockson, S., & Leifert, C. (2013) · Agronomy · doi:10.3390/agronomy3010059 · Cited by 14
Kairouani, A., Pontier, D., Picart, C., Mounet, F., Martinez, Y., Le-Bot, L., Fanuel, M., Hammann, P., Belmudes, L., Merret, R., et al. (2023) · eLife · doi:10.7554/elife.88207 · Cited by 7
Metadata
- Authors
- Hurkman, W. J., & Tanaka, C. K.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1986-07-01
- Volume
- 81
- Issue
- 3
- Pages
- 802-806
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2061765768
- Cited by
- 915
- References
- 8
- FWCI
- 6.93
- Type
- journal article
- Language
- en
- Open access
- green
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- Hurkman, W. J., & Tanaka, C. K. (1986). Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis. Plant Physiology. https://doi.org/10.1104/pp.81.3.802
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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