The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells.
Open accessAbstract
The CCAAT/enhancer-binding protein alpha (C/EBP alpha) has been implicated in the regulation of adipoblast differentiation. In this study we investigate the potential of C/EBP alpha to promote the adipogenic program in a variety of fibroblastic cells. Transduction of the C/EBP alpha gene into eight mouse fibroblastic cell lines by retroviruses and DNA transfection generates adipocyte colonies at variable frequencies. The most dramatic results are obtained with NIH-3T3 cells, in which the percentage of G418-resistant colonies that exhibit the adipocyte morphology is reproducibly > 50% when the C/EBP alpha gene is transduced by retroviruses. The ability to promote the adipogenic program requires the potent transcriptional activation domain of C/EBP alpha and is not observed with C/EBP beta. Paradoxically, in spite of its antimitogenic effects, clonal cell lines that stably express high amounts of C/EBP alpha can readily be generated. Stable expression of C/EBP alpha in BALB/c-3T3 cells dramatically enhances their ability to terminally differentiate into adipocytes. The results demonstrate that C/EBP alpha can efficiently promote the adipogenic program in a variety of mouse fibroblastic cells, including those that have little or no spontaneous capacity to undergo adipogenesis.
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Cites
The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins
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Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.
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CCAAT-Enhancer Binding Protein: A Component of a Differentiation Switch
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Cited by
Understanding Adipocyte Differentiation
GREGOIRE, F. M., SMAS, C. M., & SUL, H. S. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.3.783 · Cited by 2,376
Adipogenesis and metabolic health
Ghaben, A. L., & Scherer, P. E. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0093-z · Cited by 1,395
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Inhibition of Adipogenesis Through MAP Kinase-Mediated Phosphorylation of PPARγ
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Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation
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Metadata
- Authors
- Freytag, S. O., Paielli, D. L., & Gilbert, J. D.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1994-07-15
- Volume
- 8
- Issue
- 14
- Pages
- 1654-1663
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2025505666
- Cited by
- 456
- References
- 4
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Freytag, S. O., Paielli, D. L., & Gilbert, J. D. (1994). Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells. Genes & Development. https://doi.org/10.1101/gad.8.14.1654
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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