Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 1,141

The DOI matches its Crossref record.

Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5.

Open access
Lyons, I, Parsons, L M, Hartley, L, Li, R, Andrews, J E, Robb, L, Harvey, R P·1995·Genes & Development
DOI 10.1101/gad.9.13.1654
Open this paper in CoChat — free account

Abstract

The murine homeo box gene Nkx2-5 is expressed in precardiac mesoderm and in the myocardium of embryonic and fetal hearts. Targeted interruption of Nkx2-5 resulted in abnormal heart morphogenesis, growth retardation and embryonic lethality at approximately 9-10 days postcoitum (p.c.). Heart tube formation occurred normally in mutant embryos, but looping morphogenesis, a critical determinant of heart form, was not initiated at the linear heart tube stage (8.25-8.5 days p.c.). Commitment to the cardiac muscle lineage, expression of most myofilament genes and myofibrillogenesis were not compromised. However, the myosin light-chain 2V gene (MLC2V) was not expressed in mutant hearts nor in mutant ES cell-derived cardiocytes. MLC2V expression normally occurs only in ventricular cells and is the earliest known molecular marker of ventricular differentiation. The regional expression in mutant hearts of two other ventricular markers, myosin heavy-chain beta and cyclin D2, indicated that not all ventricle-specific gene expression is dependent on Nkx2-5. The data demonstrate that Nkx2-5 is essential for normal heart morphogenesis, myogenesis, and function. Furthermore, this gene is a component of a genetic pathway required for myogenic specialization of the ventricles.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/gad.9.13.1654

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 75

  1. 1993, Multiple regulatory elements contribute differentially to muscle creatine kinase enhancer activity in skeletal and cardiac muscle., Mol. Cell. Biol., 13, 2753
  2. 10.1101/gad.7.7b.1325 · DOI · Paper page
  3. 10.1016/0092-8674(92)90187-H · DOI · Paper page
  4. 1993, The gene tinman is required for specification of the heart and visceral muscles in Drosophila., Development, 118, 719, 10.1242/dev.118.3.719 · DOI · Paper page
  5. Bodmer, R. 1995. Heart development in Drosophila and its relationship to vertebrates. TCM (in press). · DOI · Paper page
  6. 1990, A new homeobox-containing gene, msh-2, is transiently expressed early during mesoderm formation in Drosophila., Development, 110, 661, 10.1242/dev.110.3.661 · DOI · Paper page
  7. Bradley, A. 1987. Production and analysis of chimaeric mice. In Teratocarcinomas and embryonic stem cells: A practical approach (ed. E.J. Robertson), pp. 113–151. IRL Press, Washington, DC.
  8. 10.1016/0959-437X(94)90142-P · doi:10.1016/0959-437x(94)90142-p
  9. 1994, A novel basic helix-loop-helix protein expressed in paraxial mesoderm and immature somites of mouse embryos and in heart and skeletal muscle of the adult mouse., J. Cell. Biochem., 18D, 487
  10. 10.1101/gad.8.19.2293 · DOI · Paper page
  11. 10.1016/0003-2697(87)90021-2 · DOI · Paper page
  12. 1993, Regulation of the human cardiac/slow-twitch troponin C gene by multiple, cooperative, cell-type-specific, and myoD-responsive elements., Mol. Cell. Biol., 13, 6752
  13. 1994, Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis., Development, 120, 1251, 10.1242/dev.120.5.1251 · DOI · Paper page
  14. 1994, Heterokaryons of cardiac myocytes and fibroblasts reveal the lack of dominance of the cardiac phenotype., Mol. Cell. Biol., 14, 4269
  15. 1992, M-CAT binding factor is related to the SV40 enhancer binding factor, TEF-1., J. Biol. Chem., 267, 17234, 10.1016/S0021-9258(18)41917-5 · DOI
  16. 10.1016/0092-8674(94)90292-5 · DOI · Paper page
  17. 1992, The involvement of adherens junction components in myofibrillogenesis in cultured cardiac myocytes., Development, 114, 173, 10.1242/dev.114.1.173 · DOI
  18. 1994, A hormone-encoding gene identifies a pathway for cardiac but Not skeletal muscle gene transcription., Mol. Cell. Biol., 14, 3115
  19. 10.1016/0092-8674(93)90110-C · DOI · Paper page
  20. 1991, Egr-1, a serum-inducible zinc finger protein, regulates transcription of the rat cardiac α-myosin heavy chain gene., J. Biol. Chem., 266, 12813, 10.1016/S0021-9258(18)98762-4 · DOI
  21. 10.1006/dbio.1994.1206 · DOI · Paper page
  22. 10.1007/BF00292022 · DOI
  23. 10.1038/326292a0 · DOI · Paper page
  24. 1991, Actin bundles on the right side in the caudal part of the heart tube play a role in dextro-looping in the embryonic chick heart., Anat. Embryol., 183, 29
  25. 10.1038/ng0794-353 · DOI · Paper page
  26. 1988, Features of embryonic induction., Development, 104, 341, 10.1242/dev.104.3.341 · doi:10.1242/dev.104.3.341
  27. 10.1126/science.7973707 · DOI · Paper page
  28. 10.1073/pnas.86.20.7716 · DOI · Paper page
  29. 10.1073/pnas.90.17.8145 · DOI · Paper page
  30. 1994, Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis., J. Biol. Chem., 269, 16961, 10.1016/S0021-9258(19)89483-8 · DOI
  31. 10.1093/nar/19.15.4293 · DOI · Paper page
  32. 10.1016/0955-0674(94)90037-X · DOI
  33. Layton, W.M., M.D. Manasek, and D.M.D. Manasek. 1980. Cardiac looping in early iv/iv mouse embryos. In Etiology and morphogenesis of congenital heart disease (ed. R. Van Pragh), pp. 109–126. Futura, Mount Kisco, New York.
  34. 1993, Nkx-2.5: A novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants., Development, 119, 419, 10.1242/dev.119.2.419 · DOI · Paper page
  35. 10.1006/dbio.1993.1088 · doi:10.1006/dbio.1993.1088
  36. 1993, Induction of cardiac muscle differentiation in isolated animal pole explants of Xenopus laevis embryos., Development, 118, 865, 10.1242/dev.118.3.865 · DOI
  37. 1994, In situ analysis of the cardiac muscle gene program during embryogenesis., TCM, 4, 70
  38. 10.1083/jcb.111.6.2427 · DOI · Paper page
  39. 10.1021/bi00570a013 · doi:10.1021/bi00570a013
  40. Manasek, F.J. 1976. Interactions involved in cardiac morphogenesis. In The cell surface in animal embryogenesis and development (ed. G. Poste, and G.C. Nicholson), pp. 545–598. North-Holland, Amsterdam, Holland.
  41. 10.1016/0012-1606(72)90077-2 · DOI
  42. 1978, Cyto-differentiation: A causal antecedent of looping?, Birth Defects: Original Article Series, 14, 161
  43. 10.1083/jcb.107.2.573 · DOI
  44. 1987, Duplication CArG box domains have positive and mutually dependent regulatory roles in expression of the human α-cardiac actin gene., Mol. Cell. Biol., 7, 2802
  45. 1993, Defects in heart and lung development in compound heterozygotes for two different targeted mutations at the N-myc locus., Development, 119, 485, 10.1242/dev.119.2.485 · DOI · Paper page
  46. 1993, Expression of the α-myosin heavy chain gene in the heart is regulated in part by and E-box-dependent mechanism., J. Biol. Chem., 268, 2602, 10.1016/S0021-9258(18)53817-5 · doi:10.1016/s0021-9258(18)53817-5
  47. 10.1006/dbio.1994.1180 · doi:10.1006/dbio.1994.1180
  48. 10.1016/0092-8674(89)90434-0 · DOI · Paper page
  49. 1992, A ubiquitous factor (Hf-1a) and a distinct muscle factor (Hf-1b/MEF2) form an E-box-independent pathway for cardiac muscle gene expression., Mol. Cell. Biol., 12, 1469
  50. 1994, The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements., Mol. Cell. Biol., 14, 7331
  51. 10.1073/pnas.90.11.5157 · DOI · Paper page
  52. 1994, The Caenorhabditis elegans NK-2 class homeoprotein CEH-22 is involved in combinatorial activation of gene expression in pharyngeal muscle., Development, 120, 2175, 10.1242/dev.120.8.2175 · DOI · Paper page
  53. 10.1161/01.RES.72.1.1 · DOI · Paper page
  54. 1994, bHLH factors in muscle development: Deadlines and commitment, what to leave in and what to leave out., Genes & Dev., 8, 1, 10.1101/gad.8.1.1 · DOI · Paper page
  55. 1992, Identification and characterization of a cardiac-specific transcriptional regulatory element in the slow/cardiac troponin C gene., Mol. Cell. Biol., 12, 1967
  56. 1990, Mouse embryonic stem cells express the cardiac myosin heavy chain genes during development in vitro., J. Biol. Chem., 265, 11905, 10.1016/S0021-9258(19)38485-6 · DOI
  57. 10.1016/0092-8674(93)90621-V · DOI · Paper page
  58. 10.1016/S0074-7696(08)60228-4 · DOI · Paper page
  59. 10.1101/gad.8.11.1311 · DOI · Paper page
  60. 10.1073/pnas.89.9.4047 · DOI
  61. 10.1016/0012-1606(90)90083-U · doi:10.1016/0012-1606(90)90083-u
  62. 1990, The role of the dorsal lip in induction of heart mesoderm in Xenopus laevis., Development, 108, 461, 10.1242/dev.108.3.461 · DOI
  63. 10.1126/science.8197461 · DOI · Paper page
  64. 10.1016/0092-8674(94)90001-9 · DOI
  65. 1988, The localization of laminin mRNA and protein in the postimplantation embryo and placenta of the mouse: An in situ and immunocytochemical study., Development, 104, 431, 10.1242/dev.104.3.431 · DOI
  66. 1986, Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells., Cell, 51, 503
  67. 10.1006/dbio.1994.1089 · DOI
  68. 1993, Different regulatory sequences control creatine kinase-M gene expression in directly injected skeletal and cardiac muscle., Mol. Cell. Biol., 13, 1264
  69. 10.1016/S0022-5320(73)80002-4 · DOI · Paper page
  70. 10.1073/pnas.86.14.5434 · DOI · Paper page
  71. 10.1126/science.1846704 · DOI · Paper page
  72. Wilkinson, D.G. 1992. Whole mount in situ hybridization of vertebrate embryos. In In situ hybridization: A practical approach (ed. D.G. Wilkinson), pp. 75–84. Oxford University Press, Oxford, UK.
  73. 10.1111/j.1432-0436.1991.tb00255.x · DOI · Paper page
  74. Wurst, W. and A. Joyner. 1993. Production of targeted embryonic stem cell clones. In Gene targeting a practical approach (ed. A.L. Joyner), pp. 33–61. Oxford University Press, New York.
  75. 1993, A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1B/MEF-2) in the rat myosin light-chain 2 gene., Mol. Cell. Biol., 13, 4432

Cited by

See all 1,141

Metadata

Authors
Lyons, I., Parsons, L. M., Hartley, L., Li, R., Andrews, J. E., Robb, L., & Harvey, R. P.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
1995-07-01
Volume
9
Issue
13
Pages
1654-1666
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
DOI
10.1101/gad.9.13.1654
OpenAlex
W2076600697
Cited by
1,141
References
0
Type
posted content
Language
en
Open access
diamond

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5. https://app.cochat.ai/papers/10.1101/gad.9.13.1654
The paper
Lyons, I., Parsons, L. M., Hartley, L., Li, R., Andrews, J. E., Robb, L., & Harvey, R. P. (1995). Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5. Genes & Development. https://doi.org/10.1101/gad.9.13.1654

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.