The DOI matches its Crossref record; 39 of the 40 references it cites were found in a registry or an index.
KSR is a scaffold required for activation of the ERK/MAPK module
Open accessAbstract
Mechanisms that regulate signal propagation through the ERK/MAPK pathway are still poorly understood. Several proteins are suspected to play critical roles in this process. One of these is Kinase Suppressor of Ras (KSR), a component previously identified in RAS-dependent genetic screens in Drosophila and Caenorhabditis elegans . Here, we show that KSR functions upstream of MEK within the ERK/MAPK module. In agreement with this, we found that KSR facilitates the phosphorylation of MEK by RAF. We further show that KSR associates independently with RAF and MEK, and that these interactions lead to the formation of a RAF/MEK complex, thereby positioning RAF in close proximity to its substrate MEK. These findings suggest that KSR functions as a scaffold that assembles the RAF/MEK functional pair.
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“both of which promote ERK signaling (133–136)”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [134]
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1 retracted
Kinase Suppressor of Ras Signals through Thr269 of c-Raf-1
Xing, H., & Kolesnick, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008096200 · Cited by 69
RetractedSignaling Through Scaffold, Anchoring, and Adaptor Proteins
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Mitogen-Activated Protein Kinases: Specific Messages from Ubiquitous Messengers
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Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions
Kölch, W. (2000) · Biochemical Journal · doi:10.1042/bj3510289 · Cited by 1,443
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Bmi-1 collaborates with c-Myc in tumorigenesis by inhibiting c-Myc-induced apoptosis via INK4a/ARFPreprint
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A Cytoplasmic Inhibitor of the JNK Signal Transduction Pathway
Dickens, M., Rogers, J. S., Cavanagh, J., Raitano, A., Xia, Z., Halpern, J. R., Greenberg, M. E., Sawyers, C. L., & Davis, R. J. (1997) · Science · doi:10.1126/science.277.5326.693 · Cited by 675
A Mammalian Scaffold Complex That Selectively Mediates MAP Kinase Activation
Whitmarsh, A. J., Cavanagh, J., Tournier, C., Yasuda, J., & Davis, R. J. (1998) · Science · doi:10.1126/science.281.5383.1671 · Cited by 652
Osmotic Activation of the HOG MAPK Pathway via Ste11p MAPKKK: Scaffold Role of Pbs2p MAPKK
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Kinase drug discovery 20 years after imatinib: progress and future directions
Cohen, P., Cross, D. A. E., & Janne, P. A. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00195-4 · Cited by 1,092
LKB1-Dependent Signaling Pathways
Alessi, D. R., Sakamoto, K., & Bayascas, J. R. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.75.103004.142702 · Cited by 805
Regulation of MAP Kinase Signaling Modules by Scaffold Proteins in Mammals
Morrison, D. K., & Davis, R. J. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111401.091942 · Cited by 787
Regulation of RAF protein kinases in ERK signalling
Lavoie, H., & Therrien, M. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3979 · Cited by 702
Self-citationRAF-MEK-ERK pathway in cancer evolution and treatment
Ullah, R., Yin, Q., Snell, A. H., & Wan, L. (2022) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2021.05.010 · Cited by 553
Metadata
- Authors
- Roy, F., Laberge, G., Douziech, M., Ferland-McCollough, D., & Therrien, M.
- Affiliations
- Montreal Clinical Research Institute (CA)
- Corresponding author
- Therrien, Marc
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-02-15
- Volume
- 16
- Issue
- 4
- Pages
- 427-438
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC155344
- OpenAlex
- W2099267053
- Cited by
- 217
- References
- 40
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 9 · 2023: 2 · 2022: 6 · 2021: 8 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 9 · 2012: 4
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Keywords
- KSR, MAPK/ERK pathway, MEK phosphorylation, Raf, scaffold proteins, signal transduction, Ras signaling, protein-protein interaction
- MeSH terms
- Animals, Cells, Cultured, Drosophila melanogaster, Phosphorylation, Protein Kinases, Protein Processing, Post-Translational, Recombinant Fusion Proteins, Transfection, ras Proteins, Proto-Oncogene Proteins c-raf, Mitogen-Activated Protein Kinase Kinases, MAP Kinase Signaling System, Drosophila Proteins, Macromolecular Substances
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