A Large Imprinted microRNA Gene Cluster at the Mouse Dlk1-Gtl2 Domain
Abstract
microRNAs (or miRNAs) are small noncoding RNAs (21 to 25 nucleotides) that are processed from longer hairpin RNA precursors and are believed to be involved in a wide range of developmental and cellular processes, by either repressing translation or triggering mRNA degradation (RNA interference). By using a computer-assisted approach, we have identified 46 potential miRNA genes located in the human imprinted 14q32 domain, 40 of which are organized as a large cluster. Although some of these clustered miRNA genes appear to be encoded by a single-copy DNA sequence, most of them are arranged in tandem arrays of closely related sequences. In the mouse, this miRNA gene cluster is conserved at the homologous distal 12 region. In vivo all the miRNAs that we have detected are expressed in the developing embryo (both in the head and in the trunk) and in the placenta, whereas in the adult their expression is mainly restricted to the brain. We also show that the miRNA genes are only expressed from the maternally inherited chromosome and that their imprinted expression is regulated by an intergenic germline-derived differentially methylated region (IG-DMR) located ∼200 kb upstream from the miRNA cluster. The functions of these miRNAs, which seem only conserved in mammals, are discussed both in terms of epigenetic control and gene regulation during development.
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The largest miRNA cluster discovered to date consists of more than 50 miRNAs in the human imprinted 14q32 domain, with orthologous clusters in rat (6q32) and mouse (12qF1).
“The largest miRNA cluster discovered to date consists of Ͼ50 miRNAs in the human imprinted 14q32 domain (Seitz et al., 2004), which has orthologous clusters in the rat (6q32) and mouse (12qF1).”
From Comprehensive expression analyses of neural cell-type-specific miRNAs identify new determinants of the specification and maintenance of neuronal phenotypes (2013) · cites this paper as Seitz et al., 2004
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MicroRNAs in placental health and disease
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What does genetics tell us about imprinting and the placenta connection?
Varmuza, S., & Miri, K. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1714-0 · Cited by 22 · see citing works
- Authors
- Seitz, H., Royo, H., Bortolin, M.-L., Lin, S.-P., Ferguson-Smith, A. C., & Cavaillé, J.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-08-12
- Volume
- 14
- Issue
- 9
- Pages
- 1741-1748
- ISSN
- 1088-9051
- Cited by
- 450
- References
- 1
- Language
- en
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