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Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography
Open accessAbstract
Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized to Drosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays. [The sequence data from this study have been submitted to GEO under accession nos. GPL208, GSM2409, GSM2410, GSM2411, GSM2412, GSM2413, GSM2414, GSE81, GSE82.]
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Obesity-associated improvements in metabolic profile through expansion of adipose tissue
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Direct selection of human genomic loci by microarray hybridization
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Metadata
- Authors
- Nuwaysir, E. F., Huang, W., Albert, T. J., Singh, J., Nuwaysir, K., Pitas, A., Richmond, T., Gorski, T., Berg, J. P., Ballin, J., McCormick, M., Norton, J., Pollock, T., Sumwalt, T., Butcher, L., Porter, D., Molla, M., Hall, C., Blattner, F., Sussman, M. R., Wallace, R. L., Cerrina, F., & Green, R. D.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-11-01
- Volume
- 12
- Issue
- 11
- Pages
- 1749-1755
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W2117212863
- Cited by
- 364
- References
- 10
- Type
- posted content
- Language
- en
- Open access
- bronze
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- Nuwaysir, E. F., Huang, W., Albert, T. J., Singh, J., Nuwaysir, K., Pitas, A., Richmond, T., Gorski, T., Berg, J. P., Ballin, J., McCormick, M., Norton, J., Pollock, T., Sumwalt, T., Butcher, L., Porter, D., Molla, M., Hall, C., Blattner, F., . . . Green, R. D. (2002). Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography. Genome Research. https://doi.org/10.1101/gr.362402
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