PreprintCited by 404Bioinformatics and Genomic NetworksAdvanced Proteomics Techniques and ApplicationsBacterial Genetics and Biotechnology

Large-scale identification of protein–protein interaction of Escherichia coli K-12

Open access
Arifuzzaman, Mohammad, Maeda, Maki, Itoh, Aya, Nishikata, Kensaku, Takita, Chiharu, Saito, Rintaro, Ara, Takeshi, Nakahigashi, Kenji +15 more·2006·Genome Research

Abstract

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni 2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology.

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  • High-throughput experimental techniques designed to identify interacting protein partners have results that are far from complete and often mutually inconsistent.

    “Several high-throughput experimental techniques have been designed to identify interacting protein partners23–26, but their results are far from complete and are often mutually inconsistent.”

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Authors
Arifuzzaman, M., Maeda, M., Itoh, A., Nishikata, K., Takita, C., Saito, R., Ara, T., Nakahigashi, K., Huang, H.-C., Hirai, A., Tsuzuki, K., Nakamura, S., Altaf-Ul-Amin, M., Oshima, T., Baba, T., Yamamoto, N., Kawamura, T., Ioka-Nakamichi, T., Kitagawa, M., Tomita, M., Kanaya, S., Wada, C., & Mori, H.
Affiliations
  • Japan Bioindustry Association (JP)
  • Japan Science and Technology Agency (JP)
  • Institute for Advanced Biosciences, Keio University (JP)
  • Nara Institute of Science and Technology (JP)
  • National Yang Ming University (TW)
  • Kyoto University (JP)
Venue
Genome Research
Published
2006-04-10
Volume
16
Issue
5
Pages
686-691
ISSN
1088-9051 (ISSN-L)
OpenAlex
W2137012036
Cited by
403
References
44
Citations by year
2026: 4 · 2025: 7 · 2024: 12 · 2023: 9 · 2022: 11 · 2021: 3 · 2020: 16 · 2019: 15 · 2018: 12 · 2017: 12 · 2016: 16 · 2015: 23 · 2014: 22 · 2013: 21 · 2012: 36
Type
posted content
Language
en
Open access
Locations
  • Genome Research (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
Funders
Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
protein-protein interaction, Escherichia coli K-12, pull-down assay, protein-protein interaction network, interactome networks, protein function, systems biology, drug target identification
MeSH terms
Histidine, Models, Biological, Gene Library, Open Reading Frames, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteome, Escherichia coli Proteins, Proteomics, Escherichia coli K12

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