PreprintCited by 642Genomics and Chromatin DynamicsChromosomal and Genetic VariationsDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record.

Temporal Control of Puffing Activity in Polytene Chromosomes

Ashburner, M., Chihara, C., Meltzer, P., Richards, G.·1974·Cold Spring Harbor Symposia on Quantitative Biology

DOI 10.1101/sqb.1974.038.01.070

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Abstract

The genetic organization of individual bands of polytene chromosomes is an enigma. On the one hand, we have evidence (Rudkin, 1965; Daneholt and Edström, 1967; Mulder et al, 1968; see also Rasch et al., 1971) that an “average” band contains DNA equivalent to 40,000 or so nucleotide pairs. The range is perhaps between 3000 and 100,000 nucleotide pairs (Beermann, 1972). On the other hand, classical (e.g., Painter, 1934; Bridges, 1935, 1936; Mackensen, 1935; Muller and Prokofyeva, 1935) and more modern (e.g., Hochman, 1971; Lefevre, 1971; Lim et al., 1971; Judd et al., 1972; Rayle, 1972) evidence strongly suggests that one band (or band plus adjacent interband(s), if you will: Crick, 1971) corresponds to a unit of genetic function.

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Crossref · 10.1101/sqb.1974.038.01.070

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Items 201–300 of 642

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Self-citation
Specific protein synthesis in cellular differentiation
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Background
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Method
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Background
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Method
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Background
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Background
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Self-citation
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Background
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Result
Delayed secondary glucocorticoid response elements. Unusual nucleotide motifs specify glucocorticoid receptor binding to transcribed regions of alpha 2u-globulin DNA.
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Background
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“In insect post-embryonic development, 20-hydroxyecdysone (20E), an active form of ecdysteroid, causes various ., 1993), and elicits hierarchical effects through the activation and/or inhibition of early and early–late genes (Ashburner et al. , 1974; Ashburner, 1990).”
Background
Ecdysteroid-dependent expression of a novel cuticle protein gene BMCPG1 in the silkworm, Bombyx mori
Suzuki, Y., Matsuoka, T., Iimura, Y., & Fujiwara, H. (2002) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(01)00136-9 · Cited by 40
The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription
Kotlikova, I. V., Demakova, O. V., Semeshin, V. F., et al. (2005) · Genetics · doi:10.1534/genetics.105.045286 · Cited by 41
“Temporal changes in the expression of distinct genes active in the salivary gland are accompanied by puffing of distinct chromosome regions in welldescribed patterns that mark the steps of development through larval and pupal stages (Ashburner et al. 1974; reviewed in Zhimulev 1999).”
Background
The reaction with polytene chromosomes of antibodies raised against Drosophila E75A protein
Hill, R. J., Segraves, W. A., Choi, D., et al. (1993) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(93)90087-9 · Cited by 41
Drosophila E93 promotes adult development and suppresses larval responses to ecdysone during metamorphosis
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Developmental expression of mRNAs for epidermal and fat body proteins and hormonally regulated transcription factors in the tobacco hornworm, Manduca sexta
Hiruma, K., & Riddiford, L. M. (2010) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2010.03.029 · Cited by 40
Drosophila hemolymph proteins: Purification, characterization, and genetic mapping of larval serum protein 2 in D. melanogaster
Akam, M. E., Roberts, D. B., & Wolfe, J. (1978) · Biochemical Genetics · doi:10.1007/bf00484387 · Cited by 40
Isolation, characterization and developmental expression of the ecdysteroid‐induced E75 gene of the wax moth Galleria mellonella
Jindra, M., Sehnal, F., & Riddiford, L. M. (1994) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1994.tb18779.x · Cited by 40
Separate glucocorticoid, heavy metal, and heat shock domains in thymic lymphocytes.
Maytin, E. V., & Young, D. A. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44235-9 · Cited by 40
History of Retinoic Acid Receptors
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Ultrastructural changes of Drosophila larval and prepupal salivary glands cultured in vitro with ecdysone
Farkas, R., & Šuťáková, G. (1998) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-998-0036-7 · Cited by 42
“…chromosomes (Ashburner, 1972b, 1972c, 1973, 1974; Ashburner and Richards, 1976; Richards, 1976a, 1976b, 1976c), and the combination of those findings with genetic tools (Walker and Ashburner, 1981) led to formulation of an ecdysone genetic model (Ashburner et al., 1974, 1990; Natzle, 1993).”
Background
Induction of the Acute-Phase Reactant, α1-Acid Glycoprotein, by Glucocorticoids in Rat Hepatoma Cells
Vannice, J. L., RINGOLD, G. M., McLEAN, J. W., & Taylor, J. M. (1983) · DNA · doi:10.1089/dna.1983.2.205 · Cited by 36
The hsp27 gene of the Mediterranean fruit fly, Ceratitis capitata : structural characterization, regulation and developmental expression
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“As in Drosophila (Ashburner et al., 1974), in vivo and in vitro studies in medfly salivary glands have shown that ecdysone triggers the induction of two puffing cycles; one at the larval-prepupal transition and another at the mid-prepupal stage (Gariou-Papalexiou et al., 1999, 2001).”
Background
Downregulation of the tissue-specific transcription factor Fork head byBroad-Complexmediates a stage-specific hormone response
Renault, N., King‐Jones, K., & Lehmann, M. (2001) · Development · doi:10.1242/dev.128.19.3729 · Cited by 37
Nongenomic effects of ecdysteroids
Tomaschko, K. (1999) · Archives of Insect Biochemistry and Physiology · doi:10.1002/(sici)1520-6327(1999)41:2 · Cited by 41

Authors: Ashburner, M., Chihara, C., Meltzer, P., & Richards, G.
Affiliations: University of Cambridge (GB)
ORCID: Ashburner, M.
Venue: Cold Spring Harbor Symposia on Quantitative Biology
Publisher: Cold Spring Harbor Laboratory
Published: 1974-01-01
Volume: 38
Issue: 0
Pages: 655-662
ISSN: 0091-7451 (ISSN-L)
1943-4456
DOI: 10.1101/sqb.1974.038.01.070
OpenAlex: W2136267625
Cited by: 641
References: 0
Citations by year: 2026: 6 · 2025: 7 · 2024: 10 · 2023: 7 · 2022: 4 · 2021: 7 · 2020: 9 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 9 · 2015: 5 · 2014: 12 · 2013: 11 · 2012: 14
Type: posted content
Language: en
Open access: closed
Locations: Cold Spring Harbor Symposia on Quantitative Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: polytene chromosomes, puffing, chromosome banding, genetic organization, interbands, Drosophila
MeSH terms: Animals, Anti-Bacterial Agents, Chromosomes, Cycloheximide, DNA, Drosophila melanogaster, Ecdysone, Enzyme Repression, Genes, Puromycin, RNA, Time Factors, Transcription, Genetic, Protein Biosynthesis

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Temporal Control of Puffing Activity in Polytene Chromosomes

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