The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits
Open accessAbstract
Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless fruits can be of great value for consumers, the processing industry, and breeding companies. In this article, we propose a novel strategy to obtain parthenocarpic tomatoes by down-regulation of the flavonoid biosynthesis pathway using RNA interference (RNAi)-mediated suppression of chalcone synthase (CHS), the first gene in the flavonoid pathway. In CHS RNAi plants, total flavonoid levels, transcript levels of both Chs1 and Chs2, as well as CHS enzyme activity were reduced by up to a few percent of the corresponding wild-type values. Surprisingly, all strong Chs-silenced tomato lines developed parthenocarpic fruits. Although a relation between flavonoids and parthenocarpic fruit development has never been described, it is well known that flavonoids are essential for pollen development and pollen tube growth and, hence, play an essential role in plant reproduction. The observed parthenocarpic fruit development appeared to be pollination dependent, and Chs RNAi fruits displayed impaired pollen tube growth. Our results lead to novel insight in the mechanisms underlying parthenocarpic fruit development. The potential of this technology for applications in plant breeding and biotechnology will be discussed.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
A plant DNA minipreparation: Version II
Dellaporta, S. L., Wood, J., & Hicks, J. B. (1983) · Plant Molecular Biology Reporter · doi:10.1007/bf02712670 · Cited by 7,526
REPuter: the manifold applications of repeat analysis on a genomic scale
Kurtz, S. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.22.4633 · Cited by 2,618
Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis
Schwab, R., Ossowski, S., Riester, M., Warthmann, N., & Weigel, D. (2006) · The Plant Cell · doi:10.1105/tpc.105.039834 · Cited by 1,346
How genes paint flowers and seeds
Mol, J., Grotewold, E., & Koes, R. (1998) · Trends in Plant Science · doi:10.1016/s1360-1385(98)01242-4 · Cited by 848
Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis
Brown, D. E., Rashotte, A. M., Murphy, A. S., Normanly, J., Tague, B. W., Peer, W. A., Taiz, L., & Muday, G. K. (2001) · Plant Physiology · doi:10.1104/pp.126.2.524 · Cited by 799
The flavonoid biosynthetic pathway in plants: Function and evolution
Koes, R. E., Quattrocchio, F., & Mol, J. N. M. (1994) · BioEssays · doi:10.1002/bies.950160209 · Cited by 768
Flavonoids as developmental regulators
Taylor, L. P., & Grotewold, E. (2005) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2005.03.005 · Cited by 646
The Tomato Aux/IAA Transcription Factor IAA9 Is Involved in Fruit Development and Leaf Morphogenesis
Wang, H., Jones, B., Li, Z., Frasse, P., Delalande, C., Regad, F., Chaabouni, S., Latché, A., Pech, J.-C., & Bouzayen, M. (2005) · The Plant Cell · doi:10.1105/tpc.105.033415 · Cited by 601
Pollen and Stigma Structure and Function: The Role of Diversity in Pollination
Edlund, A. F. (2004) · THE PLANT CELL ONLINE · doi:10.1105/tpc.015800 · Cited by 590
Cited by
The Flavonoid Biosynthesis Network in Plants
Liu, W., Feng, Y., Yu, S., Fan, Z., Li, X., Li, J., & Yin, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222312824 · Cited by 1,020
Metabolomics-assisted breeding: a viable option for crop improvement?
Fernie, A. R., & Schauer, N. (2009) · Trends in Genetics · doi:10.1016/j.tig.2008.10.010 · Cited by 550
Ballester, A.-R., Molthoff, J., de Vos, R., Hekkert, B. T. L., Orzaez, D., Fernaݩndez-Moreno, J.-P., Tripodi, P., Grandillo, S., Martin, C., Heldens, J., et al. (2009) · Plant Physiology · doi:10.1104/pp.109.147322 · Cited by 398
Self-citationMuhlemann, J. K., Younts, T. L. B., & Muday, G. K. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1811492115 · Cited by 367
Perspectives on deciphering mechanisms underlying plant heat stress response and thermotolerance
Bokszczanin, K. (2013) · Frontiers in Plant Science · doi:10.3389/fpls.2013.00315 · Cited by 353
Metadata
- Authors
- Schijlen, E. G. W. M., de Vos, C. H. R., Martens, S., Jonker, H. H., Rosin, F. M., Molthoff, J. W., Tikunov, Y. M., Angenent, G. C., van Tunen, A. J., & Bovy, A. G.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-05-03
- Volume
- 144
- Issue
- 3
- Pages
- 1520-1530
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2114817617
- Cited by
- 310
- References
- 31
- FWCI
- 4.31
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits. https://app.cochat.ai/papers/10.1104/pp.107.100305
- The paper
- Schijlen, E. G. W. M., de Vos, C. H. R., Martens, S., Jonker, H. H., Rosin, F. M., Molthoff, J. W., Tikunov, Y. M., Angenent, G. C., van Tunen, A. J., & Bovy, A. G. (2007). RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato Fruits. Plant Physiology. https://doi.org/10.1104/pp.107.100305
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
