The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor
Open accessAbstract
Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by BR. Through this screening experiment, the compound brassinazole was selected as the most potent chemical. In dark-grown Arabidopsis, brassinazole-induced morphological changes were nearly restored to those of wild type by treatment with brassinolide. The structure of brassinazole is similar to pacrobutrazol, a gibberellin biosynthesis inhibitor. However, in assays with cress (Lepidium sativum) plants, brassinazole-treated plants did not show recovery after the addition of gibberellin but showed good recovery after the addition of brassinolide. These data demonstrate that brassinazole is a specific BR biosynthesis inhibitor. Brassinazole-treated cress also showed dwarfism, with altered leaf morphology, including the downward curling and dark green color typical of Arabidopsis BR-deficient mutants, and this dwarfism was reversed by the application of 10 nm brassinolide. This result suggests that BRs are essential for plant growth, and that brassinazole can be used to clarify the function of BRs in plants as a complement to BR-deficient mutants. The brassinazole action site was also investigated by feeding BR biosynthesis intermediates to cress grown in the light.
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Cites
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GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation
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Wang, Z.-Y., Seto, H., Fujioka, S., Yoshida, S., & Chory, J. (2001) · Nature · doi:10.1038/35066597 · Cited by 846
Self-citationSantiago, J., Henzler, C., & Hothorn, M. (2013) · Science · doi:10.1126/science.1242468 · Cited by 357
Brassinosteroids and Plant Steroid Hormone Signaling
Bishop, G. J., & Koncz, C. (2002) · The Plant Cell · doi:10.1105/tpc.001461 · Cited by 270
Brassinosteroid‐Promoted Growth
Müssig, C. (2005) · Plant Biology · doi:10.1055/s-2005-837493 · Cited by 133
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Metadata
- Authors
- Asami, T., Min, Y. K., Nagata, N., Yamagishi, K., Takatsuto, S., Fujioka, S., Murofushi, N., Yamaguchi, I., & Yoshida, S.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-05-01
- Volume
- 123
- Issue
- 1
- Pages
- 93-100
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2115320729
- Cited by
- 396
- References
- 35
- FWCI
- 21.25
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Asami, T., Min, Y. K., Nagata, N., Yamagishi, K., Takatsuto, S., Fujioka, S., Murofushi, N., Yamaguchi, I., & Yoshida, S. (2000). Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor. Plant Physiology. https://doi.org/10.1104/pp.123.1.93
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