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Cited by 309

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Analysis of Flanking Sequences from Dissociation Insertion Lines: A Database for Reverse Genetics in Arabidopsis

Open access
Parinov, Serguei, Sevugan, Mayalagu, Ye, De, Yang, Wei-Cai, Kumaran, Mande, Sundaresan, Venkatesan·1999·The Plant Cell

Abstract

We have generated Dissociation (Ds) element insertions throughout the Arabidopsis genome as a means of random mutagenesis. Here, we present the molecular analysis of genomic sequences that flank the Ds insertions of 931 independent transposant lines. Flanking sequences from 511 lines proved to be identical or homologous to DNA or protein sequences in public databases, and disruptions within known or putative genes were indicated for 354 lines. Because a significant portion (45%) of the insertions occurred within sequences defined by GenBank BAC and P1 clones, we were able to assess the distribution of Ds insertions throughout the genome. We discovered a significant preference for Ds transposition to the regions adjacent to nucleolus organizer regions on chromosomes 2 and 4. Otherwise, the mapped insertions appeared to be evenly dispersed throughout the genome. For any given gene, insertions preferentially occurred at the 5′ end, although disruption was clearly possible at any intragenic position. The insertion sites of >500 lines that could be characterized by reference to public databases are presented in a tabular format at http://www.plantcell.org/cgi/content/full/11/12/2263/DC1. This database should be of value to researchers using reverse genetics approaches to determine gene function.

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Metadata

Authors
Parinov, S., Sevugan, M., Ye, D., Yang, W.-C., Kumaran, M., & Sundaresan, V.
Venue
The Plant Cell
Published
1999-12-01
Volume
11
Issue
12
Pages
2263-2270
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W2115094987
Cited by
309
References
23
FWCI
34.07
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Analysis of Flanking Sequences from Dissociation Insertion Lines: A Database for Reverse Genetics in Arabidopsis. https://app.cochat.ai/papers/10.1105/tpc.11.12.2263
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Parinov, S., Sevugan, M., Ye, D., Yang, W.-C., Kumaran, M., & Sundaresan, V. (1999). Analysis of Flanking Sequences from Dissociation Insertion Lines: A Database for Reverse Genetics in Arabidopsis. The Plant Cell. https://doi.org/10.1105/tpc.11.12.2263

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