Cited by 16Malaria Research and ControlEnzyme Structure and FunctionBiochemical and Structural Characterization

Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein

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Higgins, Matthew K.·2008·Acta Crystallographica Section F Structural Biology and Crystallization Communications

Abstract

The PfEMP1 proteins of the malaria parasite Plasmodium falciparum are inserted into the membrane of infected red blood cells, where they mediate adhesion to a variety of human receptors. The DBL domains of the var2csa-encoded PfEMP1 protein play a critical role in malaria of pregnancy, tethering infected cells to the surface of the placenta through interactions with the glycosaminoglycan carbohydrate chondroitin sulfate A (CSA). A CSA-binding DBL domain has been overproduced in a bacterial expression system, purified and crystallized. Native data sets extending to 1.9 A resolution have been collected and phasing is under way.

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  1. [4]4. Baruch, D. I., Gormley, J. A., Howard, R. J. & Pasloske, B. L. (1996). Proc. Natl Acad. Sci. USA, 93, 3497-3502.
  2. [8]8. Evans, P. R. (1993). Proceedings of the CCP4 Study Weekend. Data Collection and Processing, edited by L. Sawyer, N. Isaacs & S. Bailey, pp. 114-133.

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Authors
Higgins, M. K.
Affiliations
University of Cambridge (GB)
Corresponding author
Higgins, Matthew K.
Venue
Acta Crystallographica Section F Structural Biology and Crystallization Communications
Published
2008-02-29
Volume
64
Issue
3
Pages
221-223
ISSN
1744-3091 (ISSN-L)
OpenAlex
W2020862529
Cited by
16
References
23
Citations by year
2025: 1 · 2024: 1 · 2022: 1 · 2017: 1 · 2015: 3
FWCI
2.97
Type
journal article
Language
en
Open access
Locations
  • Acta Crystallographica Section F Structural Biology and Crystallization Communications (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
PfEMP1, DBL domains, VAR2CSA, chondroitin sulfate A, Plasmodium falciparum, placental adhesions, glycosaminoglycan binding, protein crystallization, recombinant protein overexpression, protein purification
MeSH terms
Animals, Antigens, Protozoan, Chondroitin Sulfates, Crystallization, Plasmodium falciparum, Protein Binding, X-Ray Diffraction, Protozoan Proteins

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