Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein
Open accessAbstract
The PfEMP1 proteins of the malaria parasite Plasmodium falciparum are inserted into the membrane of infected red blood cells, where they mediate adhesion to a variety of human receptors. The DBL domains of the var2csa-encoded PfEMP1 protein play a critical role in malaria of pregnancy, tethering infected cells to the surface of the placenta through interactions with the glycosaminoglycan carbohydrate chondroitin sulfate A (CSA). A CSA-binding DBL domain has been overproduced in a bacterial expression system, purified and crystallized. Native data sets extending to 1.9 A resolution have been collected and phasing is under way.
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Cites
Genome sequence of the human malaria parasite Plasmodium falciparum
Gardner, M. J., Hall, N., Fung, E., White, O., Berriman, M., Hyman, R. W., Carlton, J. M., Pain, A., Nelson, K. E., Bowman, S., et al. (2002) · Nature · doi:10.1038/nature01097 · Cited by 4,407 · see citing works
Adherence of <i>Plasmodium falciparum</i> to Chondroitin Sulfate A in the Human Placenta
Fried, M., & Duffy, P. E. (1996) · Science · doi:10.1126/science.272.5267.1502 · Cited by 1,108 · see citing works
Salanti, A., Staalsoe, T., Lavstsen, T., Jensen, A. T. R., Sowa, M. P. K., Arnot, D. E., Hviid, L., & Theander, T. G. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03570.x · Cited by 702 · see citing works
Maternal antibodies block malaria
Fried, M., Nosten, F., Brockman, A., Brabin, B. J., & Duffy, P. E. (1998) · Nature · doi:10.1038/27570 · Cited by 659 · see citing works
Evidence for the Involvement of VAR2CSA in Pregnancy-associated Malaria
Salanti, A., Dahlbäck, M., Turner, L., Nielsen, M. A., Barfod, L., Magistrado, P., Jensen, A. T. R., Lavstsen, T., Ofori, M. F., Marsh, K., et al. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20041579 · Cited by 604 · see citing works
Rowe, J. A., Moulds, J. M., Newbold, C., & Miller, L. H. (1997) · Nature · doi:10.1038/40888 · Cited by 580 · see citing works
Baruch, D. I., Gormely, J. A., Ma, C., Howard, R. J., & Pasloske, B. L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3497 · Cited by 399 · see citing works
Tolia, N. H., Enemark, E. J., Sim, B. K. L., & Joshua‐Tor, L. (2005) · Cell · doi:10.1016/j.cell.2005.05.033 · Cited by 331 · see citing works
Ricke, C. H., Staalsoe, T., Koram, K., Akanmori, B. D., Riley, E. M., Theander, T. G., & Hviid, L. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.6.3309 · Cited by 303 · see citing works
Chen, Q., Barragán, A., Fernandez, V., Sundström, A., Schlichtherle, M., Sahlén, A., Carlson, J., Datta, S., & Wahlgren, M. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.187.1.15 · Cited by 294 · see citing works
Duffy, P. E., & Fried, M. (2003) · Infection and Immunity · doi:10.1128/iai.71.11.6620-6623.2003 · Cited by 260 · see citing works
Structural basis for Duffy recognition by the malaria parasite Duffy-binding-like domain
Singh, S. K., Hora, R., Belrhali, H., Chitnis, C. E., & Sharma, A. (2006) · Nature · doi:10.1038/nature04443 · Cited by 247 · see citing works
Viebig, N. K., Gamain, B., Scheidig, C., Lépolard, C., Przyborski, J., Lanzer, M., Gysin, J., & Scherf, A. (2005) · The EMBO Reports · doi:10.1038/sj.embor.7400466 · Cited by 201 · see citing works
High Level of<i>var2csa</i>Transcription by<i>Plasmodium falciparum</i>Isolated from the Placenta
Tuikue Ndam, N. G., Salanti, A., Bertin, G., Dahlbäck, M., Fievet, N., Turner, L., Gaye, A., Theander, T., & Deloron, P. (2005) · The Journal of Infectious Diseases · doi:10.1086/430933 · Cited by 179 · see citing works
O'Neil-Dunne, I., Achur, R. N., Agbor-Enoh, S. T., Valiyaveettil, M., Naik, R. S., Ockenhouse, C. F., Zhou, A., Megnekou, R., Leke, R., Taylor, D. W., et al. (2001) · Infection and Immunity · doi:10.1128/iai.69.12.7487-7492.2001 · Cited by 156 · see citing works
Vogt, A. M., Barragán, A., Chen, Q., Kironde, F., Spillmann, D., & Wahlgren, M. (2003) · Blood · doi:10.1182/blood-2002-07-2016 · Cited by 150 · see citing works
Gamain, B., Trimnell, A. R., Scheidig, C., Scherf, A., Miller, L. H., & Smith, J. D. (2005) · The Journal of Infectious Diseases · doi:10.1086/428137 · Cited by 145 · see citing works
Baca, A. M., & Hol, W. G. J. (2000) · International Journal for Parasitology · doi:10.1016/s0020-7519(00)00019-9 · Cited by 143 · see citing works
Duffy, M. F., Maier, A. G., Byrne, T. J., Marty, A. J., Elliott, S. R., O’Neill, M. T., Payne, P. D., Rogerson, S. J., Cowman, A. F., Crabb, B. S., et al. (2006) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2006.03.006 · Cited by 114 · see citing works
Barfod, L., Bernasconi, N. L., Dahlbäck, M., Jarrossay, D., Andersen, P. H., Salanti, A., Ofori, M. F., Turner, L., Resende, M., Nielsen, M. A., et al. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05503.x · Cited by 110 · see citing works
Fried, M., Domingo, G. J., Gowda, C. D., Mutabingwa, T. K., & Duffy, P. E. (2006) · Experimental Parasitology · doi:10.1016/j.exppara.2005.12.003 · Cited by 107 · see citing works
MicroReview: The role of Plasmodium falciparum var genes in malaria in pregnancy
Rowe, J. A., & Kyes, S. A. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04256.x · Cited by 72 · see citing works
Barfod, L. K., Nielsen, M. A., Turner, L., Dahlbäck, M., Jensen, A. T. R., Hviid, L., Theander, T. G., & Salanti, A. (2006) · Infection and Immunity · doi:10.1128/iai.01617-05 · Cited by 59 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [4]4. Baruch, D. I., Gormley, J. A., Howard, R. J. & Pasloske, B. L. (1996). Proc. Natl Acad. Sci. USA, 93, 3497-3502.
- [8]8. Evans, P. R. (1993). Proceedings of the CCP4 Study Weekend. Data Collection and Processing, edited by L. Sawyer, N. Isaacs & S. Bailey, pp. 114-133.
Cited by
Sorted by citations
(2015) · Advances in Parasitology · doi:10.1016/bs.apar.2015.02.004 · Cited by 162 · see citing works
The Structure of a Chondroitin Sulfate-binding Domain Important in Placental Malaria
Higgins, M. K. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.c800086200 · Cited by 99 · see citing works
Self-citationDesigning a VAR2CSA-based vaccine to prevent placental malaria
Fried, M., & Duffy, P. E. (2015) · Vaccine · doi:10.1016/j.vaccine.2015.10.011 · Cited by 86 · see citing works
“Following these observations, two crystallography studies using recombinant DBL3 protein of the A4 allele confirmed binding to CSA [19,20].”
BackgroundKhunrae, P., Philip, J. M. D., Bull, D. R., & Higgins, M. K. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2009.08.027 · Cited by 61 · see citing works
Self-citationGhumra, A., Khunrae, P., Ataíde, R., Raza, A., Rogerson, S. J., Higgins, M. K., & Rowe, J. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016414 · Cited by 57 · see citing works
Self-citationHommel, M., Elliott, S. R., Soma, V., Kelly, G., Fowkes, F. J. I., Chesson, J. M., Duffy, M. F., Bockhorst, J. P., Avril, M., Müeller, I., et al. (2010) · Infection and Immunity · doi:10.1128/iai.01365-09 · Cited by 56 · see citing works
Juillerat, A., Igonet, S., Vigan-Womas, I., Guillotte-Blisnick, M., Gangnard, S., Faure, G., Baron, B., Raynal, B. D. E., Mercereau‐Puijalon, O., & Bentley, G. A. (2010) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2009.12.008 · Cited by 28 · see citing works
Joergensen, L. M., Salanti, A., Dobrilovic, T., Barfod, L. K., Hassenkam, T., Theander, T. G., Hviid, L., & Arnot, D. E. (2010) · Malaria Journal · doi:10.1186/1475-2875-9-100 · Cited by 25 · see citing works
Apicomplexan parasite adhesins: novel strategies for targeting host cell carbohydrates
Boulanger, M. J., Tonkin, M. L., & Crawford, J. (2010) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2010.08.003 · Cited by 21 · see citing works
Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain
Semblat, J., Ghumra, A., Czajkowsky, D. M., Wallis, R., Mitchell, D. A., Raza, A., & Rowe, J. A. (2015) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2015.06.001 · Cited by 14 · see citing works
Requena, P., Arévalo-Herrera, M., Menegon, M., Martínez-Espinosa, F. E., Padilla, N., Bôtto-Menezes, C., Malheiro, A., Hans, D., Castellanos, M. E., Robinson, L., et al. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.00163 · Cited by 13 · see citing works
McLean, F. E., Omondi, B. R., Diallo, N., Otoboh, S. E., Kifude, C., Abdi, A. I., Lim, R., Otto, T. D., Ghumra, A., & Rowe, J. A. (2025) · PLoS Pathogens · doi:10.1371/journal.ppat.1012434 · Cited by 8 · see citing works
SURFACE ANTIGENS AND VIRULENCE IN PLASMODIUM FALCIPARUM MALARIA
Normark, J. (2008) · Figshare · Cited by 1 · see citing works
Blomqvist, K. (2011) · Figshare
Quintana, M. D. P. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2189-9_20
- Authors
- Higgins, M. K.
- Affiliations
- University of Cambridge (GB)
- Corresponding author
- Higgins, Matthew K.
- ORCID
- Higgins, Matthew K.
- Venue
- Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Published
- 2008-02-29
- Volume
- 64
- Issue
- 3
- Pages
- 221-223
- ISSN
- 1744-3091 (ISSN-L)
- PMC
- PMC2374165
- OpenAlex
- W2020862529
- Cited by
- 16
- References
- 23
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2017: 1 · 2015: 3
- FWCI
- 2.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Acta Crystallographica Section F Structural Biology and Crystallization Communications (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- PfEMP1, DBL domains, VAR2CSA, chondroitin sulfate A, Plasmodium falciparum, placental adhesions, glycosaminoglycan binding, protein crystallization, recombinant protein overexpression, protein purification
- MeSH terms
- Animals, Antigens, Protozoan, Chondroitin Sulfates, Crystallization, Plasmodium falciparum, Protein Binding, X-Ray Diffraction, Protozoan Proteins
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