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Cited by 66Influenza Virus Research StudiesPneumonia and Respiratory InfectionsRespiratory viral infections research

Modulation of potential respiratory pathogens by pH1N1 viral infection.

Open access
Leung, R.K.-K., Zhou, J.-W., Guan, W., Li, S.-K., Yang, Z.-F., Tsui, S.K.-W.·2013·Clinical Microbiology and Infection
DOI 10.1111/1469-0691.12054
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Abstract

While much effort has been made to characterize influenza A pdm09 virus (pH1N1), the flu that was responsible for the fourth influenza pandemic, there is a lack of study on the composition of bacteria that lead to secondary infection. In this study, we recruited pneumonia patients with and without pH1N1 infection and characterized their oropharyngeal microbiota by the unbiased high-throughput sequencing method. While there were no significant differences in common bacterial pneumonia-causative agents (Acinetobacter and Streptococcus species), previously unreported Pseudomonas species equipped with chemotaxis and flagellar assembly genes significantly increased (>20-fold) in the pH1N1-infected group. Bacillus and Ralstonia species that also increased significantly (5-10-fold) were also found to possess similar signaling and motility genes. In contrast, no such genes were found in oral commensal Prevotella, Veillonella and Neisseria species, which decreased significantly, or in either Acinetobacter or 10 out of 21 Streptococcus species, including Streptococcus pneumoniae. Our results support the notion that pH1N1 infection provides a niche for previously unnoticed potential respiratory pathogens that were able to access the lower respiratory tract with weakened immunity.

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Crossref · 10.1111/1469-0691.12054

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  • Leung et al. (2013) found that the number of Pseudomonas and Bacillus in the oropharynx increased significantly after influenza virus infection, whereas the number of Prevotella, Veillonella, and Neisseria decreased significantly.

    “Leung et al. (2013) conducted an unbiased high-throughput sequencing method to analyze the oropharyngeal microorganisms of pneumonia patients with or without influenza virus infection, and found that the number of Pseudomonas and Bacillus in the oropharynx increased significantly after influenza virus infection, whereas the number of Prevotella, Veillonella, and Neisseria decreased significantly.”

    From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as Leung et al., 2013

  • Viruses can drive changes in both local microbial composition and quantity in the lungs.

    “Previous research has shown that viruses can drive changes in both local microbial composition and quantity in the lungs.”

    From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as Leung et al., 2013

  • These studies demonstrated that there was considerable inter-subject variability, highlighting the need for longitudinal studies to decipher changes following viral infection.

    “However, these studies demonstrated that there was considerable inter-subject variability, highlighting the need for longitudinal studies to decipher changes following viral infection.”

    From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [108]

  • An examination of the oropharyngeal microbiome of pneumonia patients with and without 2009 influenza A H1N1 pandemic viral infection showed that Firmicutes (which include Staphylococcus and Streptococcus spp.) and Proteobacteria (mainly Pseudomonas amygdali, P. fluorescens, Pseudomonas sp. UK4, Acinetobacter baumanii and A. junii) were significantly enriched in patients with influenza.

    “An examination of the oropharyngeal microbiome of pneumonia patients with and without 2009 influenza A H1N1 pandemic viral infection showed that Firmicutes (which include Staphylococcus and Streptococcus spp.) and Proteobacteria (mainly Pseudomonas amygdali, P. fluorescens, Pseudomonas sp. UK4, Acinetobacter baumanii and A. junii)—were significantly enriched in patients with influenza (108).”

    From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [108]

  • A metagenomic analysis showed that pH1N1-associated airway microbiotas were enriched in genes associated with cell motility, transcriptional regulation, metabolism, and response to chemotaxis compared to the same bacteria in non-infected patients.

    “A metagenomic analysis showed that pH1N1-associated airway microbiotas were enriched in genes associated with cell motility, transcriptional regulation, metabolism, and response to chemotaxis compared to the same bacteria in non-infected patients (108).”

    From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [108]

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Authors
Leung, R. K.-K., Zhou, J.-W., Guan, W., Li, S.-K., Yang, Z.-F., & Tsui, S. K.-W.
Affiliations
  • Chinese University of Hong Kong (HK)
  • State Key Laboratory of Respiratory Disease (CN)
Corresponding author
  • Yang, Z.-F.
  • Tsui, S.K.-W.
ORCID
Venue
Clinical Microbiology and Infection
Publisher
Elsevier BV
Published
2013-10
Volume
19
Issue
10
Pages
930-935
ISSN
1198-743X (ISSN-L)
DOI
10.1111/1469-0691.12054
OpenAlex
W1772185144
Cited by
66
References
30
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 4 · 2021: 14 · 2020: 6 · 2019: 1 · 2018: 9 · 2017: 6 · 2016: 2 · 2015: 7 · 2014: 4 · 2013: 1
FWCI
2.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Clinical Microbiology and Infection (publishedVersion)
  • PubMed (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
  • Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
Keywords
secondary bacterial infection, pneumonia, oropharyngeal microbiota, Pseudomonas, Bacillus, Ralstonia, chemotaxis genes, Streptococcus pneumoniae, Acinetobacter, oral commensals, respiratory pathogens, immunosuppression
MeSH terms
Adolescent, Adult, Aged, Bacteria, Chemotaxis, China, Female, Humans, Influenza, Human, Male, Middle Aged, Oropharynx, Pseudomonas, Pneumonia, Bacterial, Influenza A Virus, H1N1 Subtype, Young Adult, Coinfection, Microbiota

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