Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum
Open accessAbstract
The presence of ozone (O(3)) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O(3)-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O(3) by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O(3) with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O(3) doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O(3) exposures resulted in cumulative oxidative effects in the SC: As compared with O(3) exposures of 0 ppm (alpha-tocopherol, 8.95 +/- 1.3 pmol per mg; gamma-tocopherol, 3.00 +/- 0.3 pmol per mg; MDA, 3.69 +/- 0.3 pmol per mg), vitamin E was depleted (alpha-tocopherol, 2.90 +/- 0.6 pmol per mg, p < 0.001; gamma-tocopherol, 0.5 +/- 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 +/- 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O(3).
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Experimental animal studies have shown that exposure to ozone results in signs of oxidative stress including significant depletion of cutaneous antioxidants.
“Experimental animal studies have shown that exposure to ozone results in signs of oxidative stress including significant depletion of cutaneous antioxidants [34,35].”
From Oxidative Stress and Ageing: The Influence of Environmental Pollution, Sunlight and Diet on Skin (2017) · cites this paper as [34]
Cites
The chemistry and antioxidant properties of tocopherols and tocotrienols
Kamal‐Eldin, A., & Appelqvist, L. (1996) · Lipids · doi:10.1007/bf02522884 · Cited by 1,836 · see citing works
Epidermal Lipids, Barrier Function, and Desquamation
Elias, P. M. (1983) · Journal of Investigative Dermatology · doi:10.1038/jid.1983.12 · Cited by 1,356 · see citing works
Antioxidant defenses and lipid peroxidation in human blood plasma.
Frei, B. B., Stocker, R., & Ames, B. N. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.24.9748 · Cited by 1,254 · see citing works
Role of vitamin E in preventing the oxidation of low-density lipoprotein
Esterbauer, H., Dieber‐Rotheneder, M., Striegl, G., & Waeg, G. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.1.314s · Cited by 640 · see citing works
Pryor, W. A., Stanley, J. P., & Blair, E. (1976) · Lipids · doi:10.1007/bf02532843 · Cited by 504 · see citing works
Vitamin E in Humans: Demand and Delivery
Traber, M. G., & Sies, H. (1996) · Annual Review of Nutrition · doi:10.1146/annurev.nu.16.070196.001541 · Cited by 502 · see citing works
Self-citationAtopic dermatitis: A genetic-epidemiologic study in a population-based twin sample
Larsen, F. S. (1993) · Journal of the American Academy of Dermatology · doi:10.1016/0190-9622(93)70099-f · Cited by 498 · see citing works
Biochemical basis of ozone toxicity
Mustafa, M. G. (1990) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(90)90035-h · Cited by 386 · see citing works
Siegenthaler, U., Laine, A. M., & Polák, L. (1983) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12531034 · Cited by 352 · see citing works
The cascade mechanism to explain ozone toxicity: The role of lipid ozonation products
Pryor, W. A., Squadrito, G. L., & Friedman, M. B. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)02033-7 · Cited by 340 · see citing works
Oxidants, antioxidants, and respiratory tract lining fluids.
Cross, C. E., van der Vliet, A., O’Neill, C., Louie, S., & Halliwell, B. B. (1994) · Environmental Health Perspectives · doi:10.1289/ehp.94102s10185 · Cited by 308 · see citing works
Self-citationOptimization of Physiological Lipid Mixtures for Barrier Repair
Man, M., Feingold, K. R., Thornfeldt, C. R., & Elias, P. M. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12340135 · Cited by 294 · see citing works
Young, I. S., & Trimble, E. R. (1991) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456329102800514 · Cited by 275 · see citing works
How far does ozone penetrate into the pulmonary air/tissue boundary before it reacts?
Pryor, W. A. (1992) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(92)90060-t · Cited by 270 · see citing works
Phase behavior of isolated skin lipids
Bouwstra, J. A., Gooris, G. S., Cheng, K., Weerheim, A. M., Bras, W., & Ponec, M. (1996) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42010-3 · Cited by 216 · see citing works
Ozone: An overview of its toxicity in man and animals
Menzel, D. B. (1984) · Journal of Toxicology and Environmental Health · doi:10.1080/15287398409530493 · Cited by 212 · see citing works
Aldehydes, hydrogen peroxide, and organic radicals as mediators of ozone toxicity
Pryor, W. A., & Church, D. F. (1991) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(91)90186-7 · Cited by 170 · see citing works
Oxidation of Unsaturated Fatty Acids by Ozone and Nitrogen Dioxide
Roehm, J. N., Hadley, J. G., & Menzel, D. B. (1971) · Archives of Environmental Health An International Journal · doi:10.1080/00039896.1971.10665972 · Cited by 164 · see citing works
Ubiquinone-Dependent Recycling of Vitamin E Radicals by Superoxide
Stoyanovsky, D. A., Osipov, A. N., Quinn, P. J., & Kagan, V. E. (1995) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1995.9955 · Cited by 163 · see citing works
Lipids and the epidermal water barrier: metabolism, regulation, and pathophysiology.
Elias, P. M., & Kr, F. (1992) · PubMed · Cited by 155 · see citing works
Hydroperoxides in plants exposed to ozone mediate air pollution damage to alkene emitters
HEWITT, C. N., Kok, G. L., & Fall, R. (1990) · Nature · doi:10.1038/344056a0 · Cited by 148 · see citing works
Thiele, J. J., Traber, M. G., Tsang, K., Cross, C. E., & Packer, L. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(96)00617-x · Cited by 138 · see citing works
Self-citationKagan, V. E., Witt, E. H., Goldman, R., Scita, G., & Packer, L. (1992) · Free Radical Research Communications · doi:10.3109/10715769209049159 · Cited by 120 · see citing works
Self-citationCan vitamin E protect humans against the pathological effects of ozone in smog?
Pryor, W. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.3.702 · Cited by 118 · see citing works
Uppu, R. M., Cueto, R., Squadrito, G. L., & PRYOR, W. A. (1995) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1995.1290 · Cited by 80 · see citing works
Oxidative stress mediates synthesis of cytosolic phospholipase A2 after UVB injury
Chen, X., Gresham, A., Morrison, A. R., & Pentland, A. P. (1996) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(95)00166-2 · Cited by 80 · see citing works
Ozone depletes tocopherols and tocotrienols topically applied to murine skin.
Thiele, J. J., Traber, M. G., Podda, M., Tsang, K., Cross, C. E., & Packer, L. (1997) · FEBS Letters · doi:10.1016/s0014-5793(96)01463-9 · Cited by 79 · see citing works
Self-citationYu, L. W., Latriano, L., Duncan, S., et al. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90261-7 · Cited by 60 · see citing works
Cholesterol ozonation products as biomarkers for ozone exposure in rats
Pryor, W. A., Wang, K., & Bermúdez, E. (1992) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(92)91101-u · Cited by 54 · see citing works
[On the histotopography of cytochrome oxidase in normal and pathological skin and in skin tumors].
Braun‐Falco, O. (1961) · PubMed · Cited by 41 · see citing works
Braun‐Falco, O. (1961) · Archives of Dermatological Research · doi:10.1007/bf00515391 · Cited by 38 · see citing works
Vitamin E as a human skin penetration enhancer
Trivedi, J. S., Krill, S. L., & Fort, J. J. (1995) · European Journal of Pharmaceutical Sciences · doi:10.1016/0928-0987(95)00014-5 · Cited by 36 · see citing works
Signal Transduction Mechanisms in Photocarcinogenesis
Pentland, A. P. (1996) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1996.tb03048.x · Cited by 13 · see citing works
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Sorted by citations
Krutmann, J., Bouloc, A., Sore, G., Bernard, B. A., & Passeron, T. (2017) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2016.09.015 · Cited by 768 · see citing works
The dual action of ozone on the skin
Valacchi, G., Fortino, V., & Bocci, V. (2005) · British Journal of Dermatology · doi:10.1111/j.1365-2133.2005.06939.x · Cited by 243 · see citing works
Vitamin E in human skin: Organ-specific physiology and considerations for its use in dermatology
Thiele, J. J., & Ekanayake-Mudiyanselage, S. (2007) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2007.06.001 · Cited by 218 · see citing works
Self-citationRecognizing the impact of ambient air pollution on skin health
Mancebo, S. E., & Wang, S. Q. (2015) · Journal of the European Academy of Dermatology and Venereology · doi:10.1111/jdv.13250 · Cited by 190 · see citing works
Weber, S. U., Thiele, J. J., Packer, L., & Cross, C. E. (1999) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.1999.00789.x · Cited by 125 · see citing works
Self-citationEvaluation of the impact of urban pollution on the quality of skin: a multicentre study in Mexico
Lefebvre, M. ‐., Pham, D. ‐., Boussouira, B., Bernard, D., Camus, C., & Nguyen, Q. ‐. (2015) · International Journal of Cosmetic Science · doi:10.1111/ics.12203 · Cited by 113 · see citing works
Selenoproteins Are Essential for Proper Keratinocyte Function and Skin Development
Sengupta, A., Lichti, U. F., Carlson, B. A., Ryscavage, A. O., Gladyshev, V. N., Yuspa, S. H., & Hatfield, D. L. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012249 · Cited by 109 · see citing works
Aryl Hydrocarbon Receptor Is an Ozone Sensor in Human Skin
Afaq, F., Zaid, M. A., Pelle, E., Khan, N., Syed, D. N., Matsui, M. S., Maes, D., & Mukhtar, H. (2009) · Journal of Investigative Dermatology · doi:10.1038/jid.2009.85 · Cited by 99 · see citing works
Oxidative Stress and Ageing: The Influence of Environmental Pollution, Sunlight and Diet on Skin
Naidoo, K., & Birch-Machin, M. (2017) · Cosmetics · doi:10.3390/cosmetics4010004 · Cited by 83 · see citing works
Ushio, H., Nohara, K., & Fujimaki, H. (1999) · Toxicology Letters · doi:10.1016/s0378-4274(98)00379-8 · Cited by 68 · see citing works
Effects of environmentally realistic levels of ozone on stratum corneum function
He, Q. C., Tavakkol, A., Wietecha, K., Begum‐Gafur, R., Ansari, S. A., & Polefka, T. (2006) · International Journal of Cosmetic Science · doi:10.1111/j.1467-2494.2006.00347.x · Cited by 63 · see citing works
Self-citation
- Authors
- Thiele, J. J., Traber, M. G., Polefka, T. G., Cross, C. E., & Packer, L.
- Affiliations
- University of California, Berkeley (US)
- Colgate-Palmolive (United States) (US)
- University of California, Davis (US)
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 1997-05
- Volume
- 108
- Issue
- 5
- Pages
- 753-757
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2020829024
- Cited by
- 123
- References
- 33
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 5 · 2018: 2 · 2017: 7 · 2016: 5 · 2015: 7 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 5.51
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- ozone exposure, stratum corneum, vitamin E depletion, lipid peroxidation, malondialdehyde, oxidative stress, photochemical smog, skin permeability barrier, alpha-tocopherol, gamma-tocopherol, SKH-1 mice
- MeSH terms
- Air Pollutants, Animals, Dose-Response Relationship, Drug, Malondialdehyde, Mice, Hairless, Oxidants, Photochemical, Ozone, Skin, Vitamin E, Lipid Peroxidation, Oxidative Stress, Mice
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