Cited by 109Selenium in Biological SystemsOrganoselenium and organotellurium chemistryHair Growth and Disorders

Selenoproteins Are Essential for Proper Keratinocyte Function and Skin Development

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Sengupta, Aniruddha, Lichti, Ulrike F., Carlson, Bradley A., Ryscavage, Andrew O., Gladyshev, Vadim N., Yuspa, Stuart H., Hatfield, Dolph L.·2010·PLoS ONE

Abstract

Dietary selenium is known to protect skin against UV-induced damage and cancer and its topical application improves skin surface parameters in humans, while selenium deficiency compromises protective antioxidant enzymes in skin. Furthermore, skin and hair abnormalities in humans and rodents may be caused by selenium deficiency, which are overcome by dietary selenium supplementation. Most important biological functions of selenium are attributed to selenoproteins, proteins containing selenium in the form of the amino acid, selenocysteine (Sec). Sec insertion into proteins depends on Sec tRNA; thus, knocking out the Sec tRNA gene (Trsp) ablates selenoprotein expression. We generated mice with targeted removal of selenoproteins in keratin 14 (K14) expressing cells and their differentiated descendents. The knockout progeny had a runt phenotype, developed skin abnormalities and experienced premature death. Lack of selenoproteins in epidermal cells led to the development of hyperplastic epidermis and aberrant hair follicle morphogenesis, accompanied by progressive alopecia after birth. Further analyses revealed that selenoproteins are essential antioxidants in skin and unveiled their role in keratinocyte growth and viability. This study links severe selenoprotein deficiency to abnormalities in skin and hair and provides genetic evidence for the role of these proteins in keratinocyte function and cutaneous development.

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Authors
Sengupta, A., Lichti, U. F., Carlson, B. A., Ryscavage, A. O., Gladyshev, V. N., Yuspa, S. H., & Hatfield, D. L.
Affiliations
  • National Institutes of Health (US)
  • Brigham and Women's Hospital (US)
Corresponding author
  • Yuspa, Stuart H.
  • Hatfield, Dolph L.
Venue
PLoS ONE
Published
2010-08-18
Volume
5
Issue
8
Pages
e12249
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2140893882
Cited by
109
References
41
Citations by year
2026: 7 · 2025: 7 · 2024: 9 · 2023: 9 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 8 · 2018: 10 · 2017: 6 · 2016: 6 · 2015: 8 · 2014: 3 · 2013: 6 · 2012: 8
FWCI
6.07
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • National Institutes of Health
  • National Cancer Institute
  • National Institute of General Medical Sciences (R01GM061603)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Organoselenium and organotellurium chemistry
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
Keywords
selenoproteins, selenocysteine, keratinocytes, skin development, hair follicle morphogenesis, hair loss, selenium deficiency, keratin 14, epidermal hyperplasia, antioxidant enzymes, keratinocyte viability, knockout mice
MeSH terms
Epidermal Cells, Animals, Animals, Newborn, Antioxidants, Cell Adhesion, Cell Differentiation, Epidermis, Female, Gene Expression Regulation, Macrophages, Male, Organ Specificity, Recombination, Genetic, RNA, Messenger, RNA, Transfer, Amino Acid-Specific, Selenium, Skin, Lipid Peroxidation, Biomarkers, Keratinocytes, Gene Deletion, Reactive Oxygen Species, Hair Follicle, Cell Proliferation, Selenoproteins, Mice, Keratin-14, Gene Knockout Techniques

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