Protocols for dry DNA storage and shipment at room temperature
Open accessAbstract
The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica(®) DNAstable(®) plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at -20 °C. PCR and selective sequencing were performed over a 4-year interval to test the condition of DNA extracts. Biomatrica(®) provided better protection of DNA at 56 °C and at room temperature than trehalose and PVA, especially for diluted samples. PVA was the second best protectant after Biomatrica(®) at room temperature, whereas trehalose was the second best protectant at 56 °C. In spite of lower PCR success, the DNA stored at -20 °C yielded longer sequence reads and stronger signal, indicating that temperature is a crucial factor for DNA quality which has to be considered especially for long-term storage. Although it is premature to advocate a transition to DNA storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All three forms of DNA preservation enable shipment of dry DNA and PCR products between barcoding facilities.
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Ivanowa and Kuzmina (2013) indicate that additives are generally insufficient for long-term DNA storage; diluted DNA in trehalose solution stored for a month at room temperature granted only 46% PCR success, and 2-year preservation in Tris-buffered PVA granted 50% PCR success.
“However, Ivanowa and Kuzmina (2013) indicate that, generally, the additives are insufficient for long-term DNA storage. Diluted DNA in trehalose solution stored for a month at room temperature granted only 46% PCR success, and 2-year preservation in Tris-buffered PVA granted 50% PCR success, where PCR success was calculated as a percentage of positive wells per plate (96 samples) [42].”
From DNA Data Storage. (2023) · cites this paper as [42]
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“Diluted DNA in trehalose solution stored for a month at room temperature granted only 46% PCR success, and 2-year preservation in Tris-buffered PVA granted 50% PCR success, where PCR success was calculated as a percentage of positive wells per plate (96 samples) [42].”
- Authors
- Ivanova, N. V., & Kuzmina, M. L.
- Affiliations
- University of Guelph (CA)
- Biodiversity Institute of Ontario University of Guelph Guelph ON N1G 2W1 Canada
- Corresponding author
- Ivanova, Natalia V.
- Venue
- Molecular Ecology Resources
- Publisher
- Wiley
- Published
- 2013-06-24
- Volume
- 13
- Issue
- 5
- Pages
- 890-898
- ISSN
- 1755-098X (ISSN-L)
- 1755-0998
- PMC
- PMC3910151
- OpenAlex
- W2000031559
- Cited by
- 72
- References
- 27
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 5 · 2021: 8 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 7 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 1
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Ecology Resources (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Genome Canada
- Ontario Genomics
- Ontario Genomics Institute (2008‐OGI‐ICI‐03)
- Topics
- Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Invertebrate Taxonomy and Ecology
- Keywords
- DNA barcoding, DNA stabilization, room temperature storage, trehalose, polyvinyl alcohol, insect DNA, DNA degradation, sample shipment
- MeSH terms
- Animals, Desiccation, DNA, Insecta, Preservation, Biological, Specimen Handling, Temperature, Time Factors, Polymerase Chain Reaction, Sequence Analysis, DNA
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