Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus
Open accessAbstract
The type 1 diabetes-associated 16p13 locus contains the CLEC16A gene. Its preferential immune cell expression suggests involvement in autoimmunity. Given its elevated expression in dendritic and B cells - known professional antigen-presenting cells (APCs) - we hypothesize that C-type lectin domain family 16 member A (CLEC16A) may be involved in T cell co-stimulation and consequent activation and proliferation. We also sought to identify CLEC16A's subcellular localization. The effect of the CLEC16A knock-down (KD) on B cell co-stimulation and activation of T cells was tested in human lymphoblastoid cell lines (LCLs) by co-culture with CD4(+) T cells. T cell activation and proliferation were determined by flow-cytometric analysis of CD69 and CD25 expression and carboxyfluorescein succinimidyl ester (CFSE) dilution, respectively. CLEC16A subcellular localization in K562 cells was examined by immunofluorescence. We show that the CLEC16A KD did not affect the tested indices of lymphoblastoid cell line (LCL) APC capacity. Additionally, the percentage of activated T cells following LCL co-culture was not affected significantly by the CLEC16A KD. T cells co-cultured with KD or control LCLs also exhibited similar cell division profiles. CLEC16A co-localized with an endoplasmic reticulum (ER) marker, suggesting that it may be an ER protein. In conclusion, CLEC16A may not be involved in T cell co-stimulation. Additional studies on CLEC16A, accounting for its ER localization, are needed…
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These data are similar to those obtained upon CLEC16A siRNA knockdown in human B cells serving as antigen presenting cells.
“These data are similar to those obtained upon CLEC16A siRNA knockdown in human B cells serving as antigen presenting cells34.”
From Clec16a is Critical for Autolysosome Function and Purkinje Cell Survival (2016) · cites this paper as [34]
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- Authors
- Zouk, H., D'Hennezel, E., Du, X., Ounissi-Benkalha, H., Piccirillo, C. A., & Polychronakos, C.
- Affiliations
- Montreal Children's Hospital (CA)
- McGill University Health Centre (CA)
- Endocrine Genetics Laboratory, McGill University Health Center, Montreal Children's Hospital Research Institute, Montreal, QC, Canada
- Corresponding author
- Polychronakos, C
- Venue
- Clinical and Experimental Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-02-04
- Volume
- 175
- Issue
- 3
- Pages
- 485-497
- ISSN
- 0009-9104 (ISSN-L)
- 1365-2249
- PMC
- PMC3927909
- OpenAlex
- W1930060214
- Semantic Scholar
- d1349724f839b75b2b6449d28012313a48f20eb1
- Cited by
- 17
- References
- 45
- Citations by year
- 2025: 1 · 2023: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 1
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Clinical & Experimental Immunology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Juvenile Diabetes Research Foundation
- Fonds de Recherche en Santé du Québec
- Montreal Children's Hospital Research Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- CLEC16A, type 1 diabetes, T cell costimulation, antigen-presenting cells, B cells, dendritic cells, T cell activation, T cell proliferation, endoplasmic reticulum localization, subcellular localization, gene knockdown, lymphoblastoid cell lines, autoimmunity
- MeSH terms
- Antigen-Presenting Cells, B-Lymphocytes, Chromosomes, Human, Pair 16, Endoplasmic Reticulum, Humans, Leukocytes, Mononuclear, Lymphocyte Activation, Monosaccharide Transport Proteins, T-Lymphocytes, Coculture Techniques, K562 Cells, Protein Transport, Lectins, C-Type, Gene Knockdown Techniques, Genetic Loci
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