LC‐MS/MS‐based method for long‐term steroid profiling in human scalp hair
Abstract
BACKGROUND: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the method of choice for quantification of steroids. Human scalp hair provides the possibility to measure long-term retrospective steroid concentrations, which is especially useful for steroids with large time-dependent fluctuations in concentration, such as the glucocorticoid cortisol. AIM: We set up and validated a LC-MS/MS-based method for long-term steroid profiling, quantifying cortisol, cortisone, testosterone, androstenedione, dehydroepiandrosterone sulphate (DHEAS) and 17-α-hydroxyprogesterone (17OHP). METHOD: Hair locks were cut from the posterior vertex of healthy male and female volunteers and washed in isopropanol. Steroids were extracted using methanol, and extract was cleaned up by solid-phase extraction and measured on a Waters XEVO-TQ-S LC-MS/MS. Lower limit of quantification, precision, matrix interference and intra-individual variation were determined. RESULTS: The functional sensitivity of our steroid analysis was <1·3, <9·3, 2·3, <1·3, <15·9, 1·87 pg/mg hair for cortisol, cortisone, testosterone, androstenedione, dehydroepiandrosterone sulphate (DHEAS), and 17OHP, respectively. Measured over a 9-month period, the inter-run CVs were below 16% for all steroids. Intra-individual coefficients of variation were below 15% for all steroids, except 17OHP (19·7%). CONCLUSION: The authors present a LC-MS/MS-based method for long-term steroid profiling in human scalp hair, potentially providing novel insights by a multitude of clinical and research applications in the field of endocrinology.
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Hair analysis enables retrospective assessment of glucocorticoid concentrations because both cortisol and its inactive variant cortisone are incorporated in hair.
“This method enables retrospective assessment of glucocorticoid concentrations because both cortisol and its inactive variant cortisone are incorporated in hair (63).”
From Approach to the Patient: Diagnosis of Cushing Syndrome (2022) · cites this paper as [63]
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The Determination of Endogenous Steroids in Hair and Fur: A Systematic Review of Methodologies.
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Hair cortisol-a method to detect chronic cortisol levels in patients with Prader-Willi syndrome
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- Authors
- Noppe, G., de Rijke, Y. B., Dorst, K., van den Akker, E. L. T., & van Rossum, E. F. C.
- Affiliations
- Erasmus MC (NL)
- Division of Endocrinology Department of Internal Medicine Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands
- Department of Pediatrics Section Endocrinology Sophia Children's Hospital Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands
- ORCID
- Noppe, Gerard
- Venue
- Clinical Endocrinology
- Publisher
- Wiley
- Published
- 2015-04-27
- Volume
- 83
- Issue
- 2
- Pages
- 162-166
- ISSN
- 0300-0664 (ISSN-L)
- 1365-2265
- OpenAlex
- W1999743362
- Cited by
- 127
- References
- 23
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 12 · 2023: 9 · 2022: 8 · 2021: 23 · 2020: 14 · 2019: 16 · 2018: 10 · 2017: 10 · 2016: 10 · 2015: 5
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- scalp hair, cortisol, cortisone, testosterone, androstenedione, dehydroepiandrosterone sulfate, 17-hydroxyprogesterone, glucocorticoids, method validation, hair analysis, endocrinology
- MeSH terms
- Adult, Androstenedione, Chromatography, Liquid, Cortisone, Female, Glucocorticoids, Hair, Humans, Hydrocortisone, Male, Retrospective Studies, Scalp, Steroids, Testosterone, Reproducibility of Results, Dehydroepiandrosterone Sulfate, 17-alpha-Hydroxyprogesterone, Solid Phase Extraction, Tandem Mass Spectrometry, Limit of Detection
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