HPLC-FLU detection of cortisol distribution in human hair
Abstract
Objective Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft.Design and methods Hair sample was pulverized and incubated in 0.1 M HCl and extracted with ethyl acetate. The extraction was derived with sulfuric acid. The derivative was extracted with SPE C18 column.Results The detection limit was 1 pg/mg, the intraday and interday precisions were 7.3% and 8.5%, and average recovery was 102.5+/-13.0%. Cortisol concentrations of 32 humans' hair ranged from 3.28 to 24.83 pg/mg. There was a distinct decline in the cortisol of 1-cm segment along hair shaft.Conclusions This method showed that the precision was matched with mass spectroscopy. The cortisol in 1-cm hair segment sensitively reflects a retrospective calendar over 1 month.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Three independent published samples report cortisol decline rates per 1-cm hair segment of 2.5 pg/mg, 2.7±0.3 pg/mg, and 2.9±0.6 pg/mg.
“Three independent, published samples report cortisol decline rates per 1-cm hair segment of 2.5 pg/mg (Kirschbaum et al., 2009), 2.7±0.3 pg/mg (Gao et al., 2010), and 2.9±0.6 pg/mg (Xie et al., 2011).”
From Chronic Stress, Hair Cortisol and Depression: A Prospective and Longitudinal Study of Medical Internship (2018) · cites this paper as Gao et al., 2010
Cites
Analysis of endogenous cortisol concentrations in the hair of rhesus macaques
Davenport, M. D., Tiefenbacher, S., Lutz, C. K., Novak, M. A., & Meyer, J. S. (2006) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2006.01.005 · Cited by 659 · see citing works
Kirschbaum, C., Tietze, A., Skoluda, N., & Dettenborn, L. (2009) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2008.08.024 · Cited by 584 · see citing works
Ito, N., Ito, T., Kromminga, A., Bettermann, A., Takigawa, M., Kees, F., Straub, R. H., & Paus, R. (2005) · The FASEB Journal · doi:10.1096/fj.04-1968fje · Cited by 498 · see citing works
Detection of physiological concentrations of cortisol and cortisone in human hair
Raul, J.-S., Cirimele, V., Ludes, B., & Kintz, P. (2004) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2004.02.010 · Cited by 348 · see citing works
The relationship between stress and hair cortisol in healthy pregnant women
Kalra, S., Einarson, A., Karaskov, T., Uum, S. H. M. V., & Koren, G. (2007) · Clinical and investigative medicine · doi:10.25011/cim.v30i2.986 · Cited by 238 · see citing works
Hair analysis provides a historical record of cortisol levels in Cushing's syndrome
Thomson, S., Koren, G., Fraser, L.-A., Rieder, M., Friedman, T. C., & Van Uum, S. H. M. (2009) · Experimental and Clinical Endocrinology & Diabetes · doi:10.1055/s-0029-1220771 · Cited by 218 · see citing works
Cirimele, V., Kintz, P., Dumestre-Toulet, V., Goullé, J., & Ludes, B. (2000) · Forensic Science International · doi:10.1016/s0379-0738(99)00180-2 · Cited by 190 · see citing works
Influence of the cosmetic treatment of hair on drug testing
Jurado, C., Kintz, P., Menéndez‐González, M., et al. (1997) · International Journal of Legal Medicine · doi:10.1007/s004140050056 · Cited by 185 · see citing works
Hair Cortisol as a Potential Biologic Marker of Chronic Stress in Hospitalized Neonates
Yamada, J., Stevens, B., de Silva, N., Gibbins, S., Beyene, J., Taddio, A., Newman, C. E., & Koren, G. (2007) · Neonatology · doi:10.1159/000100085 · Cited by 176 · see citing works
Mechanisms of Drug Incorporation into Hair
Cone, E. J. (1996) · Therapeutic Drug Monitoring · doi:10.1097/00007691-199608000-00022 · Cited by 156 · see citing works
Salivary cortisol, stress and mood in healthy older adults: The Zenith study
Simpson, E. E. A., McConville, C. F., Rae, G., O’Connor, J. M., Stewart-Knox, B. J., Coudray, C., & Strain, J. (2008) · Biological Psychology · doi:10.1016/j.biopsycho.2007.12.001 · Cited by 50 · see citing works
Stout, P. R., Ropero‐Miller, J. D., Baylor, M. R., et al. (2007) · Forensic Science International · doi:10.1016/j.forsciint.2007.01.011 · Cited by 27 · see citing works
Okumura, T., Nakajima, Y., Takamatsu, T., & Matsuoka, M. (1995) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/0378-4347(95)00132-3 · Cited by 27 · see citing works
Nozaki, O., Ohata, T., Ohba, Y., et al. (1991) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/0378-4347(91)80195-i · Cited by 24 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Russell, E., Koren, G., Rieder, M., & Van Uum, S. (2012) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2011.09.009 · Cited by 1,063 · see citing works
Stress-related and basic determinants of hair cortisol in humans: a meta-analysis
Stalder, T., Steudte-Schmiedgen, S., Alexander, N., Klucken, T., Vater, A., Wichmann, S., Kirschbaum, C., & Miller, R. (2017) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2016.12.017 · Cited by 855 · see citing works
Hair cortisol, stress exposure, and mental health in humans: A systematic review
Staufenbiel, S. M., Penninx, B. W. J. H., Spijker, A. T., Elzinga, B. M., & van Rossum, E. F. C. (2013) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2012.11.015 · Cited by 736 · see citing works
Recent advances in cortisol sensing technologies for point-of-care application
Kaushik, A., Vasudev, A., Arya, S. K., Pasha, S. K., & Bhansali, S. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2013.09.060 · Cited by 316 · see citing works
Dettenborn, L., Tietze, A., Kirschbaum, C., & Stalder, T. (2012) · Stress · doi:10.3109/10253890.2012.654479 · Cited by 277 · see citing works
Clinical applications of cortisol measurements in hair
Wester, V. L., & van Rossum, E. F. C. (2015) · European Journal of Endocrinology · doi:10.1530/eje-15-0313 · Cited by 210 · see citing works
Dettenborn, L., Muhtz, C., Skoluda, N., Stalder, T., Steudte, S., Hinkelmann, K., Kirschbaum, C., & Otte, C. (2012) · Stress · doi:10.3109/10253890.2011.619239 · Cited by 181 · see citing works
Assessing cortisol from hair samples in a large observational cohort: the Whitehall II study
Abell, J. G., Stalder, T., Ferrie, J. E., Shipley, M. J., Kirschbaum, C., Kivimäki, M., & Kumari, M. (2016) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2016.07.214 · Cited by 175 · see citing works
Luo, H., Hu, X., Liu, X., Ma, X., Guo, W., Qiu, C., Wang, Y., Wang, Q., Zhang, X., Zhang, W., et al. (2012) · Biological Psychiatry · doi:10.1016/j.biopsych.2011.12.020 · Cited by 149 · see citing works
Mayer, S. E., Lopez-Duran, N. L., Sen, S., & Abelson, J. L. (2018) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2018.03.020 · Cited by 135 · see citing works
LC‐MS/MS‐based method for long‐term steroid profiling in human scalp hair
Noppe, G., de Rijke, Y. B., Dorst, K., van den Akker, E. L. T., & van Rossum, E. F. C. (2015) · Clinical Endocrinology · doi:10.1111/cen.12781 · Cited by 127 · see citing works
Hair cortisol and cortisone are decreased by natural sunlight
Wester, V. L., van der Wulp, N. R. P., Koper, J. W., de Rijke, Y. B., & van Rossum, E. F. C. (2016) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2016.06.016 · Cited by 93 · see citing works
Xie, Q., Gao, W., Li, J., Qiao, T., Jin, J., Deng, H., & Lu, Z. (2011) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.2011.706 · Cited by 85 · see citing works
Self-citation
- Authors
- Gao, W., Xie, Q., Jin, J., Qiao, T., Wang, H., Chen, L., Deng, H., & Lu, Z.
- Affiliations
- Southeast University (CN)
- Corresponding author
- Deng, Huihua
- Venue
- Clinical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 43
- Issue
- 7-8
- Pages
- 677-682
- ISSN
- 0009-9120 (ISSN-L)
- OpenAlex
- W1973977127
- Cited by
- 101
- References
- 14
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 11 · 2015: 8 · 2014: 4 · 2013: 15 · 2012: 10
- FWCI
- 4.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Keywords
- cortisol, human hair, hair cortisol, chronic stress biomarkers, hair shaft, HPLC-fluorescence detection, hair extraction, stress biomarkers
- MeSH terms
- Acetates, Adolescent, Adult, Child, Child, Preschool, Chromatography, High Pressure Liquid, Female, Hair, Humans, Hydrocortisone, Male, Middle Aged, Reproducibility of Results, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
