Non‐passaged muscle precursor cells from 32‐month old rat skeletal muscle have delayed proliferation and differentiation
Open accessAbstract
Objectives: The systemic environment and satellite cell dysfunction have been proposed as important contributors in the development of sarcopenia and impaired skeletal muscle regrowth with ageing. In the present study, we investigated effects of serum age on proliferation of muscle precursor cells (MPC s) isolated from skeletal muscles of young and old rats. Materials and methods: We examined proliferation and subsequent differentiation of non‐passaged MPC s isolated from skeletal muscles of 1‐, 3‐ and 32‐month old rats over a 72‐h time course, using a serum cross‐over design. Results and conclusions: We found no effect of serum age on MPC proliferation, but we did discover that MPCs isolated from skeletal muscle of 32‐month old rats had delayed onset of, and exit from proliferation, compared to MPCs isolated from skeletal muscle of 1‐month old rats. Delayed proliferation of MPCs from 32‐month old rats was associated with delayed p38 MAPK phosphorylation, and MyoD and p21 Cip1 protein expression. We also demonstrate that MPCs from 32‐month old rats exhibited lower levels of muscle creatine kinase m RNA compared to 1‐month old rats, but elevated levels of myogenin m RNA, when stimulated to differentiate after 36 h proliferation. These findings suggest that delayed entry and exit of the cell cycle observed in MPCs from 32‐month old rats may compromise their ability to respond to differentiation stimuli and subsequently impair myogenic potential of 32‐month old skeletal muscle, in this model.
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Muscle cells can regenerate through satellite mesenchymal progenitor cells, but their numbers fall with age, contributing to reduced muscle function.
“While muscle cells can regenerate through satellite mesenchymal progenitor cells, their numbers decline with age, which contributes to reduced muscle function88,89.”
From Sarcopenic obesity in older adults: aetiology, epidemiology and treatment strategies (2018) · cites this paper as [89]
The Introduction (page unavailable) says satellite cells are muscle progenitor cells involved in skeletal-muscle repair and growth, and that their dysfunction could contribute to sarcopenia. The Discussion (page unavailable) reports delayed proliferation of precursor cells from old rats, but our copy does not establish that satellite-cell numbers decline with age or that a decline in numbers causes reduced muscle function. (LLM, conf=0.87)
Experimental — accuracy not yet measuredAging-induced myosteatosis promotes lipotoxicity and contributes to inflammation.
“Furthermore, aging-induced myosteatosis promotes lipotoxicity (Lptx) and contributes to inflammation [49, 50].”
From The critical role of oxidative stress in sarcopenic obesity (2021) · cites this paper as [50]
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- Authors
- Zwetsloot, K. A., Childs, T. E., Gilpin, L. T., & Booth, F. W.
- Affiliations
- Appalachian State University (US)
- University of Missouri (US)
- Venue
- Cell Proliferation
- Publisher
- Wiley
- Published
- 2012-12-21
- Volume
- 46
- Issue
- 1
- Pages
- 45-57
- ISSN
- 0960-7722 (ISSN-L)
- 1365-2184
- PMC
- PMC3540166
- OpenAlex
- W2042183830
- Cited by
- 35
- References
- 51
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2022: 7 · 2021: 2 · 2020: 3 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 2
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Cell Proliferation (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- NC Digital Online Collection of Knowledge and Scholarship (The University of North Carolina at Greensboro) (submittedVersion)
- Figshare (submittedVersion)
- Appalachian State University
- Funders
- University of Missouri
- National Institutes of Health
- National Institute on Aging (R01AG018780, R01 AG18780)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- muscle precursor cells, satellite cells, skeletal muscle aging, sarcopenia, myogenesis, p38 MAPK phosphorylation, MyoD, p21, myogenin, creatine kinase, cell cycle, myogenic potential, rats
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Creatine Kinase, Male, Phosphorylation, Rats, Inbred F344, RNA, Messenger, Time Factors, MyoD Protein, Muscle, Skeletal, Myoblasts, p38 Mitogen-Activated Protein Kinases, Cell Proliferation, Cyclin-Dependent Kinase Inhibitor p21, Rats
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