Label‐free imaging of intracellular structures in living mammalian cells via external apodization phase‐contrast microscopy
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Abstract
Developing techniques to visualize intracellular structures, which influence the spatiotemporal functionality of biomolecules, is essential for elucidating mechanisms governing cellular behavior. In this study, we demonstrate that label‐free external apodized phase‐contrast (ExAPC) microscopy serves as a valuable tool for the simultaneous observation of various intracellular structures with high spatiotemporal resolution, while successfully mitigating halo artifacts. Additionally, through quantitative analysis of images obtained by combining ExAPC microscopy with fluorescence microscopy, we identified distinct heterogeneities in biomolecular condensates, lipid droplets, and mitochondria. Our findings highlight the potential of ExAPC microscopy to provide detailed insights into alterations in intracellular structures associated with diverse cellular processes, corroborating the existing knowledge and potentially contributing to the discovery of previously unknown cellular mechanisms.
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- Authors
- Ohno, H., Nishimura, T., Kainoh, K., Ohashi, Y., Onodera, N., Kano, M., Sari, L. N., Masuda, M., Tamura, Y., Nishino, T., Hayashi, Y., Yamamoto, Y., Takahashi, S., Mishima, Y., Yoneyama, Y., Takeuchi, Y., Sekiya, M., Matsuzaka, T., Miyamoto, T., & Shimano, H.
- Affiliations
- University of Tsukuba (JP)
- The University of Tokyo (JP)
- Toyo University (JP)
- National Cancer Centre Japan (JP)
- Tokyo Medical and Dental University (JP)
- Corresponding author
- Miyamoto, Takafumi
- ORCID
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2025-10-17
- Volume
- 293
- Issue
- 4
- Pages
- 1127-1149
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- PMC
- PMC12914761
- OpenAlex
- W4415286773
- Cited by
- 2
- References
- 74
- Citations by year
- 2026: 1 · 2025: 1
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- FEBS Journal (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Funders
- Core Research for Evolutional Science and Technology (JPMJCR1927)
- Japan Science and Technology Agency (JPMJPF2017)
- Takeda Science Foundation (DGM06005J)
- Japan Society for the Promotion of Science (KAKENHI (22H02296))
- Cabinet Office, Government of Japan
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Digital Holography and Microscopy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- label-free imaging, intracellular structures, biomolecular condensates, lipid droplets, mitochondria, live-cell imaging, spatiotemporal resolution
- MeSH terms
- Biomolecular Condensates, Animals, HeLa Cells, Humans, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Mitochondria, Lipid Droplets
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