Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells
Open accessAbstract
Hair follicle formation and cycling involve extensive and continuous interactions between epithelial and mesenchymal components. A system for rapidly and reproducibly generating hair follicles from dissociated epithelial and mesenchymal cells is described here. The system serves both as a tool for measuring the trichogenic property of cells and as a tool for studying the mechanisms that dissociated cells use to assemble an organ. In this system, hair follicles develop when dissociated cells, isolated from newborn mouse skin, are injected into adult mouse truncal skin. This morphogenetic process involves the aggregation of epithelial cells to form clusters that are sculpted by apoptosis to generate "infundibular cysts". From the "infundibular cysts", hair germs form centrifugally followed by follicular buds and then pegs that grow asymmetrically to differentiate into cycling mature pilosebaceous structures. Marker studies correlate the molecular differentiation of these follicles with in situ systems. This study suggests that the earliest phase of a developing epithelial-mesenchymal system--even from dissociated cell preparations--requires an epithelial platform.
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HF-bearing cysts arise when dissociated embryonic skin from various mammals, including humans, is subcutaneously transplanted in mice.
“Likewise, HF-bearing cysts arise when dissociated embryonic skin from various mammals, including humans, is subcutaneously transplanted in mice (Zheng et al., 2005b; 2010).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Zheng et al., 2005b
The Results and “Patch assay follicles cycle” sections report that dissociated skin cells from newborn mice produced hair follicles growing from infundibular cystic structures after injection into mouse skin. They do not establish the claim for embryonic skin from various mammals, including humans. The Results also say that injection into the deep subcutis produced few to no follicles. Page numbers are unavailable in our copy. (LLM, conf=0.90)
Experimental — accuracy not yet measuredThe mouse is a standard model for investigating skin development and disease in vivo.
“The mouse is a standard model for investigating skin development and disease in vivo”
From Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells (2022) · cites this paper as [17]
The Results (page unavailable) describe an in vivo mouse assay in which injected skin-derived cells formed hair follicles, supporting the use of mice to investigate an aspect of skin development. The cited paper does not establish that mice are a standard model for skin research generally, or report an investigation of skin disease. (LLM, conf=0.83)
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Cites
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Tissue Engineering and Regenerative Medicine: History, Progress, and Challenges
Berthiaume, F., Maguire, T. J., & Yarmush, M. L. (2011) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-061010-114257 · Cited by 734 · see citing works
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Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids
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Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells
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- Authors
- Zheng, Y., Du, X., Wang, W., Boucher, M., Parimoo, S., & Stenn, K. S.
- Corresponding author
- Stenn, Kurt S.
- ORCID
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2005-05
- Volume
- 124
- Issue
- 5
- Pages
- 867-876
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W1975847309
- Cited by
- 232
- References
- 1
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 7 · 2023: 12 · 2022: 9 · 2021: 14 · 2020: 11 · 2019: 12 · 2018: 14 · 2017: 16 · 2016: 15 · 2015: 10 · 2014: 6 · 2013: 22 · 2012: 12
- FWCI
- 8.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Keywords
- hair cycle, dissociated cells, epithelial-mesenchymal interactions, infundibular cyst, hair germ, apoptosis, mouse skin
- MeSH terms
- Animals, Cell Differentiation, Male, Mice, Inbred C57BL, Morphogenesis, Skin, Hair Follicle, Mice
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