GENETIC TRANSFORMATION OF THE DIATOMS CYCLOTELLA CRYPTICA AND NAVICULA SAPROPHILA
Abstract
Two species of diatoms were genetically transformed by introducing plasmid vectors containing the Escherichia coli neomycin phosphotransferase II (npt II)gene. Expression of the bacterial npt II gene in the diatoms was achieved using the putative promoter and terminator sequences from the acetyl‐CoA carboxylase gene from the centric diatom Cyclotella cryptica T13L Reimann, Lewin, and Guillard. The vectors were introduced into C. cryptica and the pennate diatom Navicula saprophila NAVIC1 Lange‐Bertalot and Bonik by microprojectile bombardment. Putative transformants were selected based on their ability to grow in the presence of the antibiotic G418, and production of the neomycin phosphotransferase protein by the transformed cells was confirmed by western blotting. The foreign DNA integrated into one or more random sites within the genome of the transformed algal cells, often in the form of tandem repeats. This is the first report of reproducible, stable genetic transformation of a chlorophyll c‐containing alga.
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Whole-genome sequences have been released for Thalassiosira pseudonana and Phaeodactylum tricornutum, and stable nuclear transformation has been shown for these two species and a few other diatoms.
“Whole-genome sequences have been released for two species, Thalassiosira pseudonana and Phaeodactylum tricornutum, and recent work has shown stable nuclear transformation of these two species and a few other diatoms [91–94].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [92]
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- Authors
- Dunahay, T. G., Jarvis, E. E., & Roessler, P. G.
- Affiliations
- National Laboratory of the Rockies (US)
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 1995-12
- Volume
- 31
- Issue
- 6
- Pages
- 1004-1012
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W2062892053
- Cited by
- 306
- References
- 35
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 11 · 2022: 14 · 2021: 12 · 2020: 8 · 2019: 20 · 2018: 12 · 2017: 27 · 2016: 20 · 2015: 20 · 2014: 17 · 2013: 17 · 2012: 19
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Phycology (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Diatoms and Algae Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protist diversity and phylogeny
- Keywords
- Cyclotella cryptica, neomycin phosphotransferase II, plasmid vectors, particle bombardment, G418 selection, tandem repeats, foreign DNA integration
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