Incorporation of multicellular spheroids into 3‐D polymeric scaffolds provides an improved tumor model for screening anticancer drugs
Open accessAbstract
Development of cancer therapeutics requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Three‐dimensional (3‐D) in vitro models have therefore been investigated for drug screening. In this study, we have developed a novel in vitro model in which multicellular aggregates, or spheroids, were incorporated into 3‐D porous scaffolds. Drug resistance assays showed that spheroid‐seeded scaffolds have much higher drug resistance than monolayer cultures, spheroids on flat substrates, or scaffolds seeded with dispersed cells. Furthermore, spheroid‐seeded scaffolds demonstrated higher lactate production leading to acidosis, and higher expression of angiogenic factors. These data suggest that the spheroid‐seeded 3‐D scaffolds might serve as a useful in vitro system for screening cancer therapeutics. (Cancer Sci 2010; 101: 2637–2643)
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HepG2 cells can cluster over time, but dispersed cells take longer to form cell-cell interactions when encapsulated in a porous scaffold compared to spheroids, which could affect their resistance to drugs.
“HepG2 cells can cluster over time, but dispersed cells take longer to form these cell-cell interactions when encapsulated in a porous scaffold compared to spheroids, which could affect their resistance to drugs [49].”
From A liver-on-a-chip platform with bioprinted hepatic spheroids (2016) · cites this paper as [49]
The section ‘Spheroids remain intact in scaffolds after seeding’ (page unavailable) compares dispersed cells with spheroids in porous scaffolds and reports greater drug resistance for the spheroid-seeded scaffolds. However, the experiments used U251 glioma cells, not HepG2 cells, and the copy does not establish that dispersed cells take longer than spheroids to form cell-cell interactions in scaffolds. (LLM, conf=0.86)
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- Authors
- Ho, W. J., Pham, E. A., Kim, J. W., Ng, C. W., Kim, J. H., Kamei, D. T., & Wu, B. M.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Wu, Benjamin M.
- Venue
- Cancer Science
- Publisher
- Wiley
- Published
- 2010-09-17
- Volume
- 101
- Issue
- 12
- Pages
- 2637-2643
- ISSN
- 1347-9032 (ISSN-L)
- 1349-7006
- PMC
- PMC11158092
- OpenAlex
- W2094280173
- Cited by
- 115
- References
- 46
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 13 · 2017: 11 · 2016: 11 · 2015: 8 · 2014: 8 · 2013: 6 · 2012: 4
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cancer Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- tumor spheroids, in vitro tumor model, anticancer drug screening, drug resistance, lactic acid production, tumor acidosis, cancer therapeutics
- MeSH terms
- Drug Screening Assays, Antitumor, Humans, Tumor Cells, Cultured, Spheroids, Cellular, Tissue Scaffolds
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