Cited by 95Allergic Rhinitis and SensitizationFood Allergy and Anaphylaxis ResearchAdvanced Drug Delivery Systems

Allergen‐loaded biodegradable poly(d,l‐lactic‐co‐glycolic) acid nanoparticles down‐regulate an ongoing Th2 response in the BALB/c mouse model

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Schöll, I., Weissenböck, A., Förster‐Waldl, E., Untersmayr, E., Walter, F., Willheim, M., Boltz‐Nitulescu, G., Scheiner, O. +2 more·2004·Clinical & Experimental Allergy

Abstract

Summary: Background and objective Biocompatible and biodegradable microparticles have gained interest as antigen delivery systems during the recent years. We investigated whether biodegradable poly(d,l‐lactic‐co‐glycolic) acid (PLGA) nanospheres could be used as allergen vehicles for few‐shot therapy of type I allergy. Methods The major birch pollen allergen Bet v 1 was encapsulated in PLGA nanospheres (PLGA‐Bet v 1). We examined the antigenicity and the immune response to PLGA‐Bet v 1 in a BALB/c mouse model. Results The antigenicity of Bet v 1 was largely unaffected by PLGA entrapment. When BALB/c mice were immunized subcutaneously with PLGA‐Bet v 1, they formed allergen‐specific IgG antibodies, but did not develop hypersensitivity to Bet v 1, as shown by type I skin tests. To evaluate their therapeutic potential, PLGA‐Bet v 1 with or without Al(OH)3 or non‐entrapped Bet v 1 with Al(OH)3 were used for single‐shot treatment of sensitized mice. Both groups treated with PLGA‐Bet v 1 developed high levels of Bet v 1‐specific IgG2a antibodies (P<0.01), whereas IgG1 levels decreased significantly (P<0.01). Moreover, T cells from mice treated with PLGA‐Bet v 1 showed IFN‐γ and IL‐10 production. The synthesis of these cytokines was enhanced in the groups where Al(OH)3 had been added to the vaccine formulation. Conclusion Allergen‐loaded PLGA nanoparticles modulate an ongoing Th2 response in the BALB/c mouse model, as demonstrated by down‐regulation of IgG1 and production of IFN‐γ and IL‐10. Our data strongly suggest that PLGA nanospheres can advantageously be used for formulations of allergen extracts or allergen derivatives for the few‐shot treatment of type I allergy.

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Crossref · 10.1111/j.1365-2222.2004.01884.x

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Authors
Schöll, I., Weissenböck, A., Förster‐Waldl, E., Untersmayr, E., Walter, F., Willheim, M., Boltz‐Nitulescu, G., Scheiner, O., Gabor, F., & Jensen‐Jarolim, E.
Affiliations
University of Vienna (AT)
Corresponding author
Jensen‐Jarolim, E.
Venue
Clinical & Experimental Allergy
Publisher
Wiley
Published
2004-02
Volume
34
Issue
2
Pages
315-321
ISSN
  • 0954-7894 (ISSN-L)
  • 1365-2222
OpenAlex
W2050131389
Cited by
95
References
45
Citations by year
2024: 5 · 2023: 4 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 11 · 2016: 3 · 2015: 7 · 2014: 6 · 2013: 3 · 2012: 10
FWCI
2.49
Type
journal article
Language
en
Open access
Locations
  • Clinical & Experimental Allergy (publishedVersion)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
  • Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords
PLGA nanospheres, Bet v 1, birch pollen allergen, type I allergy, allergen-specific IgG1, IgG2a, interferon-gamma, IL-10, adjuvants, aluminum hydroxide, allergen immunotherapy, antigen delivery
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Allergens, Animals, Antibodies, Biodegradation, Environmental, Desensitization, Immunologic, Female, Hypersensitivity, Immunoglobulin G, Mice, Inbred BALB C, Plant Proteins, Polyglycolic Acid, Polymers, Skin Tests, T-Lymphocytes, Th2 Cells, Lactic Acid, Nanotubes, Mice, Antigens, Plant

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