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Cited by 51Electrolyte and hormonal disordersNeuroendocrine regulation and behaviorNeuropeptides and Animal Physiology

THE DEVELOPMENT OF A RADIOIMMUNOASSAY FOR THE MEASUREMENT OF HUMAN PLASMA ARGININE VASOPRESSIN

BAYLIS, P. H., HEATH, D. A.·1977·Clinical Endocrinology
DOI 10.1111/j.1365-2265.1977.tb01300.x
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Abstract

SUMMARY: A radioimmunoassay for AVP capable of measuring human plasma AVP is described, Iodination was performed by the chloramine T method and purified by chromatography on Sephadex G‐25. Specific activity of 125 I‐AVP was 1710×155 Ci/mmol. Antiserum of high affinity (Keq= 2.7×10 11 l/mol) has been raised in rabbits, which shows slight cross‐reactivity with LVP and negligible reactivity with oxytocin. The aqueous assay is capable of detecting 0.4 fmol of AVP/tube and it is highly reproducible. A Florisil extraction technique is described in detail and gives recovery of 70% of synthetic AVP added to plasma over a wide physiological range. The lowest detectable concentration of plasma AVP is 0.3 pmol/l. The method has been validated by studying changes in plasma AVP concentration following overnight dehydration (plasma AVP = 3.46×1.89 (SD) pmol/l), and water loading (plasma AVP = 1.54×0.59 pmol/l), P < 0.005, in normal subjects. A A highly significant positive correlation has been found between plasma AVP and plasma osmolality (r=+0.75). Plasma AVP concentration has also been determined in patients with DI and the syndrome of inappropriate ADH secretion. No effect was found on the level of plasma AVP in normally hydrated volunteers undergoing postural change but levels rose following strenuous exercise from basal concentrations of 1.57×0.59 pmol/l to 4.77×3.43 pmol/l, P < 0.01.

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Crossref · 10.1111/j.1365-2265.1977.tb01300.x

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  • Baylis and Heath emphasised that even small increases in protein concentration lower binding and careful control of extract protein concentration is needed to avoid misinterpretation that a fall in binding is due to immunoreactive vasopressin.

    “When describing their vasopressin assay, Baylis and Heath 91 emphasised that ‘even small increases in protein concentration lower binding and therefore careful control of extract protein concentration is needed to avoid the misinterpretation that a fall in binding is due to immunoreactive vasopressin’.”

    From Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers (2016) · cites this paper as [91]

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Authors
BAYLIS, P. H., & HEATH, D. A.
Affiliations
Queen Elizabeth Hospital Birmingham (GB)
Corresponding author
BAYLIS, P. H.
Venue
Clinical Endocrinology
Publisher
Wiley
Published
1977-08
Volume
7
Issue
2
Pages
91-102
ISSN
  • 0300-0664 (ISSN-L)
  • 1365-2265
DOI
10.1111/j.1365-2265.1977.tb01300.x
OpenAlex
W2018439734
Cited by
51
References
27
Citations by year
2023: 1 · 2021: 1 · 2019: 1 · 2016: 1 · 2014: 1
FWCI
9.36
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 11186 d)
Locations
  • Clinical Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
University of Birmingham
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
vasopressin, radioimmunoassay, iodination, chloramine-T, plasma osmolality, dehydration, water loading, diabetes insipidus, syndrome of inappropriate antidiuretic hormone secretion, physical activity, postural changes
MeSH terms
Arginine Vasopressin, Dehydration, Diabetes Insipidus, Physical Exertion, Humans, Immune Sera, Osmolar Concentration, Posture, Radioimmunoassay, Vasopressins

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