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Cited by 787Electrolyte and hormonal disordersNeuroendocrine regulation and behaviorClinical Laboratory Practices and Quality Control

Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma

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Robertson, Gary L., Mahr, Ermelinda A., Athar, Shahid, Sinha, Tushar·1973·Journal of Clinical Investigation
DOI 10.1172/jci107423PMC PMC333039
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Abstract

A radioimmunoassay has been developed that permits reliable measurements of plasma arginine vasopressin (AVP) at concentrations as low as 0.5 pg/ml in sample volumes of 1 ml or less. Nonhormonal immunoreactivity associated with the plasma proteins is eliminated by acetone precipitation before assay, leaving unaltered a component that is immunologically and chromatographically indistinguishable from standard AVP. Storage of plasma results in a decline in AVP concentration and, thus, must be carefully regulated. The plasma AVP values obtained by our method approximate the anticipated levels and vary in accordance with physiologic expections. In recumbent normal subjects, plasma AVP ranged from (mean +/-SD) 5.4+/-3.4 pg/ml after fluid deprivation to 1.4+/-0.8 pg/ml after water loading, and correlated significantly with both plasma osmolality (r=0.52; P<0.001) and urine osmolality (r=0.77; P<0.001). After fluid restriction, plasma AVP was uniformly normal relative to plasma osmolality in patients with nephrogenic diabetes insipidus and primary polydipsia but was distinctly subnormal in all patients with pituitary diabetes insipidus. The infusion of physiologic amounts of posterior pituitary extract caused a dose-related rise in plasma vasopressin that afterwards declined at the expected rate (t(1/2)=22.5+/-4 min). We conclude that, when used appropriately, our radioimmunoassay method provides a useful way of assessing AVP function in man.

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  • Robertson et al. found that two vasopressin radioimmunoassays on unextracted plasma yielded values at least two orders of magnitude higher than those inferred from other evidence, and these values did not fluctuate in parallel with endogenous authentic vasopressin.

    “Robertson et al. showed that two radioimmunoassays for vasopressin yielded measurements in unextracted plasma that were at least two orders of magnitude greater than those inferred from other evidence, and which did not fluctuate in parallel with endogenous authentic vasopressin (33,58).”

    From Intranasal Oxytocin: Myths and Delusions (2016) · cites this paper as [58]

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Research works printed but not linked: 3

  1. Lauson, H. B. 1960. Vasopressin and oxytocin in the plasma of man and other mammals. In Hormones In Human Plasma. H. N. Antoniades, editor. Little, Brown and Company, Boston. 1st edition. 225.
  2. Verney, E. B. 1947. The antidiuretic hormone and fac- tors which determine its release. Proc. R. Soc. Lond. B. Biol. Sci. 135: 25.
  3. Share, L. 1969. Extracellular fluid volume and vaso- pressin secretion. In Frontiers in Neuroendocrinology. WV. F. Ganong and L. Martini, editors. The Oxford University Press, London. 1st edition. 183.

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Authors
Robertson, G. L., Mahr, E. A., Athar, S., & Sinha, T.
Affiliations
Larue Carter Hospital (US)
ORCID
Robertson, Gary L.
Venue
Journal of Clinical Investigation
Publisher
American Society for Clinical Investigation
Published
1973-09-01
Volume
52
Issue
9
Pages
2340-2352
ISSN
0021-9738 (ISSN-L)
DOI
10.1172/jci107423
PMC
PMC333039
OpenAlex
W1984984454
Cited by
787
References
26
Citations by year
2026: 7 · 2025: 10 · 2024: 15 · 2023: 11 · 2022: 17 · 2021: 11 · 2020: 13 · 2019: 10 · 2018: 13 · 2017: 14 · 2016: 25 · 2015: 14 · 2014: 18 · 2013: 11 · 2012: 15
FWCI
23.58
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Electrolyte and hormonal disorders
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords
vasopressin, radioimmunoassay, acetone precipitation, plasma osmolality, urine osmolality, fluid deprivation, water loading, nephrogenic diabetes insipidus, primary polydipsia, posterior pituitary extract
MeSH terms
Acetone, Antibodies, Antigen-Antibody Reactions, Arginine, Blood Preservation, Blood Pressure, Chromatography, Gel, Chromatography, Ion Exchange, Cross Reactions, Diabetes Insipidus, Diuresis, Humans, Iodine Isotopes, Lysine, Methods, Osmolar Concentration, Oxytocin, Pituitary Hormones, Posterior, Radioimmunoassay, Trimethoprim, Urine, Vasopressins, Vasotocin

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