The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Precise excision of TTAA‐specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera
Abstract
Transposon mutagenesis of baculoviruses provides an ideal experimental system for analysis of the movement of a unique family of mobile element identified from lepidopteran genomes. Members of this family of short‐inverted‐repeat elements are characterized by their extreme Specificity for TTAA target sites. This report describes the analysis of excision events for two representatives of this family, tagalong (formerly TFP3) and piggysac (formerly IFP2). These elements were tagged with a polyhedrin/lacZ reporter gene and inserted back into the virus genome either by homologous recombination or by transposition. Revertants were selected based on a white plaque phenotype. Both elements excise in a precise fashion from their positions in the baculovirus genome in either TN‐368 cells or IPLB‐SF21 AE cells. The precise excision of these elements in infected IPLB‐SF21 AE cells occurs in the absence of either tagalong or piggysac element encoded functions. The common characteristics of both insertion and excision for these elements provides further validation for their inclusion In a single family of unique transposons.
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Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
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Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
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Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
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Genetics of Mosquito Vector Competence
Beerntsen, B. T., James, A. A., & Christensen, B. M. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.1.115-137.2000 · Cited by 425
Metadata
- Authors
- Fraser, M. J., Clszczon, T., Elick, T., & Bauser, C.
- Venue
- Insect Molecular Biology
- Publisher
- Wiley
- Published
- 1996-05
- Volume
- 5
- Issue
- 2
- Pages
- 141-151
- ISSN
- 0962-1075 (ISSN-L)
- 1365-2583
- OpenAlex
- W2110637060
- Cited by
- 301
- References
- 28
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Precise excision of TTAA‐specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. https://app.cochat.ai/papers/10.1111/j.1365-2583.1996.tb00048.x
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- Fraser, M. J., Clszczon, T., Elick, T., & Bauser, C. (1996). Precise excision of TTAA‐specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Molecular Biology. https://doi.org/10.1111/j.1365-2583.1996.tb00048.x
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