The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
CsrA post‐transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli
Abstract
Summary: The RNA‐binding protein CsrA represses biofilm formation, while the non‐coding RNAs CsrB and CsrC activate this process by sequestering CsrA. We now provide evidence that the pgaABCD transcript, required for the synthesis of the polysaccharide adhesin PGA (poly‐β‐1,6‐N‐acetyl‐d‐glucosamine) of Escherichia coli, is the key target of biofilm regulation by CsrA. csrA disruption causes an approximately threefold increase in PGA production and an approximately sevenfold increase in expression of a pgaA′–′lacZ translational fusion. A Δ csrB Δ csrC mutant exhibits a modest decrease in pgaA′–′lacZ expression, while the response regulator UvrY, a transcriptional activator of csrB and csrC, stimulates this expression. Biofilm formation is not regulated by csrA, csrB or uvrY in a Δ pgaC mutant, which cannot synthesize PGA. Gel mobility shift and toeprint analyses demonstrate that CsrA binds cooperatively to pgaA mRNA and competes with 30S ribosome subunit for binding. CsrA destabilizes the pgaA transcript in vivo. RNA footprinting and boundary analyses identify six apparent CsrA binding sites in the pgaA mRNA leader, the most extensive arrangement of such sites in any mRNA examined to date. Substitution mutations in CsrA binding sites overlapping the Shine–Dalgarno sequence and initiation codon partially relieve repression by CsrA. These studies define the crucial mechanisms, though not the only means, by which the Csr system influences biofilm formation.
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Cites
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Cited by
Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules
Cress, B. F., Englaender, J. A., He, W., Kasper, D., Linhardt, R. J., & Koffas, M. A. G. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12056 · Cited by 282
“All rights reserved exopolysaccharide of E. coli, commonly secreted by many other bacteria as well, is the adhesin poly-b-1,6-GlcNAc, a homopolymer that encourages adherence and biofilm formation (Wang et al., 2004, 2005).”
BackgroundHigh-throughput mapping of the phage resistance landscape in E. coli
Mutalik, V. K., Adler, B. A., Rishi, H. S., Piya, D., Zhong, C., Koskella, B., Kutter, E. M., Calendar, R., Novichkov, P. S., Price, M. N., et al. (2020) · PLOS Biology · doi:10.1371/journal.pbio.3000877 · Cited by 171
The Role of Exopolysaccharides in Oral Biofilms
Cugini, C., Shanmugam, M., Landge, N., & Ramasubbu, N. (2019) · Journal of Dental Research · doi:10.1177/0022034519845001 · Cited by 124
Kirby, A. E., Garner, K., & Levin, B. R. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.06480-11 · Cited by 72
The role of RNA regulators, quorum sensing and c‐di‐GMP in bacterial biofilm formation
Condinho, M., Carvalho, B., Cruz, A., Pinto, S. N., Arraiano, C. M., & Pobre, V. (2023) · FEBS Open Bio · doi:10.1002/2211-5463.13389 · Cited by 64
Biofilm formation-defective mutants in Pseudomonas putida
López-Sánchez, A., Leal-Morales, A., Jiménez-Díaz, L., Platero, A. I., Bardallo-Pérez, J., Díaz-Romero, A., Acemel, R. D., Illán, J. M., Jiménez-López, J., & Govantes, F. (2016) · FEMS Microbiology Letters · doi:10.1093/femsle/fnw127 · Cited by 31
Shanmugam, M., Oyeniyi, A. O., Parthiban, C., Gujjarlapudi, S. K., Pier, G. B., & Ramasubbu, N. (2017) · Molecular Oral Microbiology · doi:10.1111/omi.12188 · Cited by 8
Metadata
- Authors
- Wang, X., Dubey, A. K., Suzuki, K., Baker, C. S., Babitzke, P., & Romeo, T.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2005-04-29
- Volume
- 56
- Issue
- 6
- Pages
- 1648-1663
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1579607832
- Cited by
- 304
- References
- 6
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- closed
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