The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea‐predominant irritable bowel syndrome
Open accessAbstract
Background The intestinal microbiota has been implicated in the pathophysiology of irritable bowel syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are inconsistent. We analyzed the composition of the intestinal microbiota in a defined subgroup of IBS patients (diarrhea‐predominant IBS, D‐IBS) using a technique that provides the deepest characterization available for complex microbial communities. Methods Fecal DNA was isolated from 23 D‐IBS patients and 23 healthy controls (HC). Variable regions V1–V3 and V6 of the 16S rRNA gene were amplified from all samples. PCR products were sequenced using 454 high throughput sequencing. The composition, diversity and richness of microbial communities were determined and compared between D‐IBS and HC using the quantitative insights into microbial ecology pipeline. Key Results The contribution of bacterial groups to the composition of the intestinal microbiota differed between D‐IBS and HC. D‐IBS patients had significantly higher levels of Enterobacteriaceae (P = 0.03), and lower levels of Fecalibacterium genera (P = 0.04) compared to HC. β‐Diversity values demonstrated significantly lower levels of UniFrac distances in HC compared to D‐IBS patients. The richness of 16S rRNA sequences was significantly decreased in D‐IBS patients (P < 0.04). Conclusions & Inferences Our 16S rRNA sequence data demonstrates a community‐level dysbiosis in D‐IBS. The altered composition of the intestinal microbiota in D‐IBS is associated with significant increases in detrimental and decreases in beneficial bacterial groups, and a reduction in microbial richness.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“others have shown that the colonic microbiome is altered in patients with IBS, when compared with healthy controls. 15 - 18”
From Efficacy of Probiotics in Irritable Bowel Syndrome: Systematic Review and Meta-analysis (2023) · cites this paper as [16]
Experimental — accuracy not yet measuredIn the citing paper
“Nonetheless, emerging data support the existence of dysbiosis in a subset of IBS [59] : a decreased complexity in composition [60] , temporary instability [61] , and changes in the mucosa-associated microbiota, with an increase in Bacteroides and Clostridia and a reduction in Bifidobacterium [62] .”
From Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis (2014) · cites this paper as [60]
In the citing paper
“Recent studies using these advanced molecular biology techniques have demonstrated quantitative alterations of specific bacterial groups (33, 34, 81, 85, 142, 154) and reduced diversity in gut microbial populations in patients with IBS compared with healthy controls (33, 40).”
From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [34]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
QIIME allows analysis of high-throughput community sequencing data
Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., Fierer, N., Peña, A. G., Goodrich, J. K., Gordon, J. I., et al. (2010) · Nature Methods · doi:10.1038/nmeth.f.303 · Cited by 34,887
Search and clustering orders of magnitude faster than BLAST
Edgar, R. C. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq461 · Cited by 21,098
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
Wang, Q., Garrity, G. M., Tiedje, J. M., & Cole, J. R. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.00062-07 · Cited by 20,145
An obesity-associated gut microbiome with increased capacity for energy harvest
Turnbaugh, P. J., Ley, R. E., Mahowald, M. A., Magrini, V., Mardis, E. R., & Gordon, J. I. (2006) · Nature · doi:10.1038/nature05414 · Cited by 12,444
Bergey's Manual of Determinative Bacteriology
S., F. A., Buchanan, R. E., Gibbons, N. E., & Bergey (1975) · Taxon · doi:10.2307/1218353 · Cited by 11,304
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
Lozupone, C., & Knight, R. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.12.8228-8235.2005 · Cited by 8,227
Diversity of the Human Intestinal Microbial Flora
Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S. R., Nelson, K. E., & Relman, D. A. (2005) · Science · doi:10.1126/science.1110591 · Cited by 7,913
A core gut microbiome in obese and lean twins
Turnbaugh, P. J., Hamady, M., Yatsunenko, T., Cantarel, B. L., Duncan, A., Ley, R. E., Sogin, M. L., Jones, W. J., Roe, B. A., Affourtit, J. P., et al. (2009) · Nature · doi:10.1038/nature07540 · Cited by 7,823
Changes of Intestinal Functions in Liver Cirrhosis
Fukui, H., & Wiest, R. (2016) · Inflammatory Intestinal Diseases · doi:10.1159/000444436 · Cited by 6,820
Host-Bacterial Mutualism in the Human Intestine
Bäckhed, F., Ley, R. E., Sonnenburg, J. L., Peterson, D. A., & Gordon, J. I. (2005) · Science · doi:10.1126/science.1104816 · Cited by 5,277
Research works printed but not linked: 1
- Ringel Y, 2008, Netter’s Textbook of Internal Medicine, 419
Cited by
Sorted by citations
Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286
Altered profile of human gut microbiome is associated with cirrhosis and its complications
Bajaj, J. S., Heuman, D. M., Hylemon, P. B., Sanyal, A. J., White, M. B., Monteith, P., Noble, N. A., Unser, A. B., Daita, K., Fisher, A. R., et al. (2014) · Journal of Hepatology · doi:10.1016/j.jhep.2013.12.019 · Cited by 1,099
Bäckhed, F., Fraser, C. M., Ringel, Y., Sanders, M. E., Sartor, R. B., Sherman, P. M., Versalovic, J., Young, V., & Finlay, B. B. (2012) · Cell Host & Microbe · doi:10.1016/j.chom.2012.10.012 · Cited by 836
Self-citationColonocyte metabolism shapes the gut microbiota
Litvak, Y., Byndloss, M. X., & Bäumler, A. J. (2018) · Science · doi:10.1126/science.aat9076 · Cited by 789
Gut Microbiota in Patients With Irritable Bowel Syndrome—A Systematic Review
Pittayanon, R., Lau, J. T., Yuan, Y., Leontiadis, G. I., Tse, F., Surette, M., & Moayyedi, P. (2019) · Gastroenterology · doi:10.1053/j.gastro.2019.03.049 · Cited by 719
Metadata
- Authors
- Carroll, I. M., Ringel‐Kulka, T., Siddle, J. P., & Ringel, Y.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Ringel, Y.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2012-02-20
- Volume
- 24
- Issue
- 6
- Pages
- 521
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- PMC
- PMC3975596
- OpenAlex
- W1487811474
- Cited by
- 425
- References
- 63
- Citations by year
- 2026: 12 · 2025: 18 · 2024: 28 · 2023: 30 · 2022: 39 · 2021: 40 · 2020: 33 · 2019: 35 · 2018: 30 · 2017: 32 · 2016: 31 · 2015: 27 · 2014: 37 · 2013: 25 · 2012: 7
- FWCI
- 12.33
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (R03DK084294, DK075621, K23DK075621, K01DK092330, DK084294)
- National Center for Research Resources (UL1RR025747)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Gastroenterology › Microscopic Colitis
- Keywords
- diarrhea-predominant irritable bowel syndrome, gut microbiota, dysbiosis, microbial diversity, microbial richness, Enterobacteriaceae, 16S rRNA gene sequencing
- MeSH terms
- Adult, Aged, Diarrhea, DNA, Bacterial, Feces, Female, Humans, Intestines, Male, Middle Aged, Irritable Bowel Syndrome, Metagenome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
