Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema
Abstract
Background Prior to the discovery of filaggrin (FLG) mutations, evidence for an impaired skin barrier in atopic dermatitis (AD) has been documented, and changes in ceramide profile, altered skin pH and increased trans-epidermal water loss (TEWL) in patients with AD have been reported. Until now, no studies have analysed stratum corneum (SC) lipids combined with skin barrier parameters in subjects of known FLG genotype. Methods A cohort of 49 German individuals genotyped for the most common FLG mutations (R501X, 2282del4) had SC samples taken for lipid analysis by high-performance thin layer chromatography. In addition, TEWL, erythema, skin hydration and pH were measured. In 27 of the 49 individuals, a 24-h irritation patch test with sodium lauryl sulphate was performed. For the analysis, both the AD group and the control group were stratified by FLG mutation status (FLGmut/FLGwt). Results In the FLGmut AD group, significantly lower levels of ceramide 4 and significantly higher levels of ceramide 7 were observed when compared to both healthy control groups. However, ceramide 7 levels also significantly differed between FLGwt AD and FLGwt controls, as did ceramide 1 levels. No significant differences were observed for ceramide 2, 3, 5 and 6. FLGmut individuals had significantly higher skin pH values than individuals not carrying FLG mutations. Patients with AD with FLG mutations had significantly higher erythema compared to patients with AD without FLG mutations. Conclusion Our…
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This reduction occurs in both affected and unaffected skin, especially in those with filaggrin abnormalities.
“This reduction occurs in both affected and unaffected skin, especially in those with filaggrin abnormalities.”
From Update on the pathogenesis of atopic dermatitis. (2024) · cites this paper as [56]
A reduction in the relationship between ceramide and cholesterol was identified in the stratum corneum of these patients.
“Furthermore, a reduction in the relationship between ceramide and cholesterol was identified in the stratum corneum of these patients.”
From Update on the pathogenesis of atopic dermatitis. (2024) · cites this paper as [56]
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“FLG mutations are the best known causes of impaired skin barrier and is considered as predisposing factors for AD.2,31,32 Many genes have been associated with AD and these genes include components of the epidermal skin barrier.33 R510X and 2282del4 are the most common mutant alleles associated with AD in Northern Euro…”
BackgroundInfluentialLipid abnormalities and lipid-based repair strategies in atopic dermatitis
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Update on the pathogenesis of atopic dermatitis.
Criado, P. R., Miot, H. A., Bueno-Filho, R., Ianhez, M., Criado, R. F. J., & de Castro, C. C. S. (2024) · Anais brasileiros de dermatologia · doi:10.1016/j.abd.2024.06.001 · Cited by 66 · see citing works
- Authors
- Jungersted, J. M., Scheer, H., Mempel, M., Baurecht, H., Cifuentes, L., Høgh, J. K., Hellgren, L. I., Jemec, G. B. E., Agner, T., & Weidinger, S.
- Affiliations
- University of Copenhagen (DK)
- Technical University of Munich (DE)
- Technical University of Denmark (DK)
- Helmholtz Munich (DE)
- Corresponding author
- Jungersted, J. M.
- Venue
- Allergy
- Publisher
- Wiley
- Published
- 2010-06
- Volume
- 65
- Issue
- 7
- Pages
- 911-918
- ISSN
- 0105-4538 (ISSN-L)
- 1398-9995
- OpenAlex
- W1916016072
- Cited by
- 344
- References
- 2
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 25 · 2023: 10 · 2022: 22 · 2021: 26 · 2020: 17 · 2019: 13 · 2018: 26 · 2017: 19 · 2016: 18 · 2015: 17 · 2014: 34 · 2013: 25 · 2012: 31
- FWCI
- 37.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Allergy (publishedVersion)
- PubMed (publishedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- Site cant be reached (submittedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- atopic dermatitis, filaggrin mutations, stratum corneum lipids, skin barrier function, transepidermal water loss, skin pH, erythema, skin hydration
- MeSH terms
- Filaggrin Proteins, Ceramides, Chromatography, High Pressure Liquid, Dermatitis, Atopic, Female, Humans, Hydrogen-Ion Concentration, Intermediate Filament Proteins, Lipids, Male, Middle Aged, Mutation, Skin
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