Oral epithelium–Candida glabrata interactions in vitro
Abstract
BACKGROUND: Oropharyngeal candidiasis is a common opportunistic infection and Candida glabrata is the second or third most frequently isolated species from oropharyngeal candidiasis lesions, after Candida albicans. The aim of this study was to study the cytokine-inducing and cell-damaging potential of C. glabrata in oral epithelial cells and compare this to C. albicans. METHODS: Oral epithelial cell lines and primary gingival epithelial cells were cocultured with C. glabrata strains GDH2269 and 94-11 or C. albicans strains SC5314 and ATCC28366. Supernatants were analysed for the presence of interleukin-1alpha (IL-1alpha), IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF) by enzyme-linked immunosorbent assay. The cytotoxity of different strains was determined using the CytoTox-96 assay. RESULTS: Compared to C. albicans, C. glabrata induced different proinflammatory cytokine responses in oral epithelial cells; a high level of GM-CSF induction was only detected in C. glabrata-infected cells and not in C. albicans-infected cells, regardless of the origin of these cells (cell lines or primary cells) or the strain used. Like C. albicans, C. glabrata induced an IL-1alpha response by oral epithelial cells, but this response was both strain-dependent and epithelial cell origin-dependent. Unlike C. albicans, C. glabrata failed to induce a strong IL-8 response in any of the cell systems studied. Finally, in these studies C. glabrata showed lower cytotoxicity than C. albicans. CONCLUSIONS: C. glabrata is less cytotoxic than C. albicans and induces different proinflammatory cytokine responses in oral epithelial cells.
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C. glabrata caused no significant cytotoxicity in oral epithelial cells.
“Unlike C. albicans, C. glabrata elicited production of the granulocyte monocyte colony-stimulating factor (GM-CSF) in oral epithelial cells but caused no significant cytotoxicity [129].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [129]
C. glabrata elicited production of GM-CSF in oral epithelial cells.
“Unlike C. albicans, C. glabrata elicited production of the granulocyte monocyte colony-stimulating factor (GM-CSF) in oral epithelial cells but caused no significant cytotoxicity [129].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [129]
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- Authors
- Li, L., & Dongari‐Bagtzoglou, A.
- Affiliations
- University of Connecticut (US)
- Corresponding author
- Dongari‐Bagtzoglou, A.
- Venue
- Oral Microbiology and Immunology
- Publisher
- Wiley
- Published
- 2007-04-30
- Volume
- 22
- Issue
- 3
- Pages
- 182-187
- ISSN
- 0902-0055 (ISSN-L)
- 1399-302X
- OpenAlex
- W2151125586
- Cited by
- 29
- References
- 41
- Citations by year
- 2024: 1 · 2022: 2 · 2020: 1 · 2019: 4 · 2018: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oral Microbiology and Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (R01 DE 13986, R01DE013986)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › HIV/AIDS oral health manifestations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Candida glabrata, Candida albicans, oropharyngeal candidiasis, oral epithelial cells, gingival epithelial cells, inflammatory cytokines, interleukin-1 alpha, interleukin-8, GM-CSF, cytotoxicity, host-pathogen interaction, strain-dependent response
- MeSH terms
- Candida albicans, Cells, Cultured, Gingiva, Humans, L-Lactate Dehydrogenase, Mouth Mucosa, Species Specificity, Keratinocytes, Granulocyte-Macrophage Colony-Stimulating Factor, Cytokines, Interleukin-8, Cell Death, Coculture Techniques, Candida glabrata, Interleukin-1alpha
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