Oral lactoferrin protects against experimental candidiasis in mice
Open accessAbstract
AIMS: To determine the role of human lactoferrin (hLF) in protecting the oral cavities of mice against Candida albicans infection in lactoferrin knockout (LFKO(-/-)) mice was compared to wild-type (WT) mice. We also aim to determine the protective role of hLF in LFKO(-/-) mice. METHODS AND RESULTS: Antibiotic-treated immunosuppressed mice were inoculated with C. albicans (or sham infection) by oral swab and evaluated for the severity of infection after 7 days of infection. To determine the protective role of hLF, we added 0·3% solution of hLF to the drinking water given to some of the mice. CFU count, scoring of lesions and microscopic observations were carried out to determine the severity of infection. LFKO(-/-) I mice showed a 2 log (P = 0·001) higher CFUs of C. albicans in the oral cavity compared to the WT mice infected with C. albicans (WTI). LFKO(-/-) I mice given hLF had a 3 log (P = 0·001) reduction in CFUs in the oral cavity compared to untreated LFKO(-/-) I mice. The severity of infection, observed by light microscopy, revealed that the tongue of the LFKO(-/-) I mice showed more white patches compared to WTI and LFKO(-/-) I + hLF mice. Scanning electron microscopic observations revealed that more filiform papillae were destroyed in LFKO(-/-) I mice when compared to WTI or LFKO(-/-) I + hLF mice. CONCLUSIONS: Human LF is important in protecting mice from oral C. albicans infection. Administered hLF may be used to prevent C. albicans infection. SIGNIFICANCE AND IMPACT OF THE STUDY: Human LF, a multifunctional iron-binding glycoprotein can be used as a therapeutic active ingredient in oral healthcare products against C. albicans.
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Administration of the glycoprotein lactoferrin can be a valid prospect for C. albicans treatment through sequestration of iron and direct toxicity, and this led to the development of a mucoadhesive tablet containing lactoferrin for treatment of oropharyngeal candidosis.
“Administration of the glycoprotein lactoferrin can be a valid prospect for C. albicans treatment through not only sequestration of iron, but also direct toxicity and this led to the development of a mucoadhesive tablet containing lactoferrin for the treatment of oropharyngeal candidosis (Kuipers et al., 2002; Bai et al., 2006; Velliyagounder et al., 2015).”
From Iron at the Centre of Candida albicans Interactions (2018) · cites this paper as Velliyagounder et al., 2015
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- Velliyagounder K Ganeshnarayan K Furgang D Fine D Lactoferrin Exhibits Proteolytic Activity Against Aae Aggregatibacter actinomycetemcomitans 2010 AADR conference March 3–6 Washington DC
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Iron at the Centre of Candida albicans Interactions
Fourie, R., Kuloyo, O. O., Mochochoko, B. M., Albertyn, J., & Pohl, C. H. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00185 · Cited by 118 · see citing works
Puri, S., Kumar, R., Rojas, I. G., Salvatori, O., & Edgerton, M. (2019) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02152-18 · Cited by 48 · see citing works
- Authors
- Velliyagounder, K., Alsaedi, W., Alabdulmohsen, W., Markowitz, K., & Fine, D. H.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Corresponding author
- Velliyagounder, K.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-11-25
- Volume
- 118
- Issue
- 1
- Pages
- 212-221
- ISSN
- 1364-5072 (ISSN-L)
- PMC
- PMC4469205
- OpenAlex
- W2035860800
- Cited by
- 27
- References
- 59
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH NIDCR (R21 DE019548)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › HIV/AIDS oral health manifestations
- Keywords
- human lactoferrin, Candida albicans, oral candidiasis, oral candidiasis prevention, iron-binding glycoprotein, filiform papillae
- MeSH terms
- Administration, Oral, Animals, Anti-Bacterial Agents, Candida albicans, Candidiasis, Humans, Lactoferrin, Male, Mouth Diseases, Tongue, Mice, Knockout, Mice
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