Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori
Abstract
Nongenomic action of an insect steroid hormone, 20‐hydroxyecdysone (20E), has been implicated in several 20E‐dependent events including the programmed cell death of Bombyx anterior silk glands (ASGs), but no information is available for the mode of the action. We provide evidence for a putative membrane receptor located in the plasma membrane of the ASGs. Membrane fractions prepared from the ASGs exhibit high binding activity to [3 H]ponasterone A (PonA). The membrane fractions did not contain conventional ecdysone receptor as revealed by Western blot analysis using antibody raised against Bombyx ecdysone receptor A (EcR‐A). The binding activity was not solubilized with 1 m NaCl or 0.05% (w/v) MEGA‐8, indicating that the binding sites were localized in the membrane. Differential solubilization and temperature‐induced phase separation in Triton X‐114 showed that the binding sites might be integrated membrane proteins. These results indicated that the binding sites are located in plasma membrane proteins, which we putatively referred to as membrane ecdysone receptor (mEcR). The mEcR exhibited saturable binding for [3 H]PonA (Kd = 17.3 n m, Bmax = 0.82 pmol·mg−1 protein). Association and dissociation kinetics revealed that [3 H]PonA associated with and dissociated from mEcR within minutes. The combined results support the existence of a plasmalemmal ecdysteroid receptor, which may act in concert with the conventional EcR in various 20E‐dependent developmental events.
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The plasma membrane can bind [3H] ponasterone A, suggesting the existence of an unknown membrane receptor.
“The plasma membrane can bind [3H] ponasterone A ([3H] Pon A), suggesting the existence of an unknown membrane receptor [39].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [39]
Several GPCRs bind with 20E, such as DmDopEcR binding with [3H]Pon A, or an unknown GPCR in the anterior silk gland of silkworms binding with [3H]Pon A.
“Numerous studies have reported the binding of several GPCRs with 20E, such as the binding of DmDopEcR with [3H]Pon A [24], or an unknown GPCR in the anterior silk gland of silkworms binding with [3H]Pon A [22].”
From G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane (2014) · cites this paper as [22]
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- Authors
- Elmogy, M., Iwami, M., & Sakurai, S.
- Affiliations
- Kanazawa University (JP)
- ORCID
- Iwami, Masafumi
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 2004-07-06
- Volume
- 271
- Issue
- 15
- Pages
- 3171-3179
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2148692431
- Cited by
- 57
- References
- 37
- Citations by year
- 2025: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 5.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- National Institute for Basic Biology
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Silkworms and Sericulture Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- 20-hydroxyecdysone, non-genomic steroid action, Bombyx mori, programmed cell death, receptor kinetics, insect steroid hormones
- MeSH terms
- Animals, Binding Sites, Cell Membrane, Ecdysone, Ecdysterone, Kinetics, Membrane Proteins, Polyethylene Glycols, Protein Binding, Receptors, Steroid, Bombyx, Solubility, Subcellular Fractions, Blotting, Western, Octoxynol
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