Cited by 30Neurobiology and Insect Physiology ResearchEstrogen and related hormone effectsReceptor Mechanisms and Signaling

The Steroid Hormone 20-Hydroxyecdysone Enhances Gene Transcription through the cAMP Response Element-binding Protein (CREB) Signaling Pathway

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Jing, Yu-Pu, Wang, Di, Han, Xiao-Lin, Dong, Du-Juan, Wang, Jin-Xing, Zhao, Xiao-Fan·2016·Journal of Biological Chemistry

Abstract

Animal steroid hormones regulate gene transcription through genomic pathways by binding to nuclear receptors. These steroid hormones also rapidly increase intracellular calcium and cyclic adenosine monophosphate (cAMP) levels and activate the protein kinase C (PKC) and protein kinase A (PKA) nongenomic pathways. However, the function and mechanism of the nongenomic pathways of the steroid hormones are unclear, and the relationship between the PKC and PKA pathways is also unclear. We propose that the steroid hormone 20-hydroxyecdysone (20E) activates the PKA pathway to enhance 20E-induced gene transcription in the lepidopteran insect Helicoverpa armigera The expression of the catalytic subunit 1 of PKA (PKAC1) increased during metamorphosis, and PKAC1 knockdown blocked pupation and repressed 20E-responsive gene expression. 20E regulated PKAC1 phosphorylation at threonine 200 and nuclear translocation through an ecdysone-responsive G-protein-coupled receptor 2. PKAC1 induced cAMP response element-binding protein (CREB) phosphorylation at serine 143, which bound to the cAMP response element on DNA to enhance 20E-responsive gene transcription. Through ecdysone-responsive G-protein-coupled receptor 2, 20E increased cAMP levels, which induced CREB PKA phosphorylation and 20E-responsive gene expression. This study demonstrates that the PKA/CREB pathway tightly and critically regulates 20E-induced gene transcription as well as its relationship with the 20E-induced PKC pathway.

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Crossref · 10.1074/jbc.m115.706028

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Authors
Jing, Y.-P., Wang, D., Han, X.-L., Dong, D.-J., Wang, J.-X., & Zhao, X.-F.
Affiliations
Shandong University (CN)
Corresponding author
Zhao, Xiao-Fan
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2016-06
Volume
291
Issue
24
Pages
12771-12785
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2340838087
Cited by
30
References
76
Citations by year
2025: 3 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 5
FWCI
2.62
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China
  • National Key Research and Development Program of China
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
20-hydroxyecdysone, ecdysone, CREB, protein kinase A, non-genomic steroid action, gene transcription, Helicoverpa armigera, metamorphosis, pupation, protein kinase C, CREB phosphorylation, cyclic AMP
MeSH terms
Animals, Cell Line, Ecdysterone, Larva, Metamorphosis, Biological, Moths, Phosphorylation, Protein Binding, Protein Kinase C, Transcription, Genetic, Blotting, Western, Signal Transduction, Cyclic AMP Response Element-Binding Protein, Cyclic AMP-Dependent Protein Kinases, Gene Expression Regulation, Developmental, Insect Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference

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