G-protein-coupled receptor controls steroid hormone signaling in cell membrane
Open accessAbstract
G-protein-coupled receptors (GPCRs) are involved in animal steroid hormone signaling, but their mechanism is unclear. In this research, we report that a GPCR called ErGPCR-2 controls steroid hormone 20-hydroxyecdysone (20E) signaling in the cell membrane of the lepidopteran insect Helicoverpa armigera. ErGPCR-2 was highly expressed during molting and metamorphosis. 20E, via ErGPCR-2, regulated rapid intracellular calcium increase, protein phosphorylation, gene transcription, and insect metamorphosis. ErGPCR-2 was located in the cell surface and was internalized by 20E induction. GPCR kinase 2 participated in 20E-induced ErGPCR-2 phosphorylation and internalization. The internalized ErGPCR-2 was degraded by proteases to desensitize 20E signaling. ErGPCR-2 knockdown suppressed the entrance of 20E analog [(3)H] ponasterone A ([(3)H]Pon A) into the cells. ErGPCR-2 overexpression or blocking of ErGPCR-2 internalization increased the entrance of [(3)H]Pon A into the cells. However, ErGPCR-2 did not bind to [(3)H]Pon A. Results suggest that ErGPCR-2 transmits steroid hormone 20E signaling and controls 20E entrance into cells in the cell membrane.
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ErGPCR-1 and ErGPCR-2 are 20E-responsive GPCRs revealed in Helicoverpa armigera.
“The 20E-responsive GPCR (initially designated ErGPCR, and later, ErGPCR-1) [47] and ErGPCR-2 [48] are further revealed in Helicoverpa armigera.”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [48]
ErGPCR-2 has a similar function to ErGPCR-1, such as regulation of rapid increases in intracellular Ca2+ levels and phosphorylation of USP.
“ErGPCR-2 has a similar function to ErGPCR-1, such as regulation of rapid increases in intracellular Ca2+ levels, and phosphorylation of USP [48].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [48]
[3H] Pon A entry into cells relies on ErGPCR-2 localization in the cell membrane.
“[3H] Pon A entry into cells relies on ErGPCR-2 localization in the cell membrane [48].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [48]
ErGPCR-2 in the lepidopteran H. armigera increases 20E entering cells.
“ErGPCR-2 in the lepidopteran H. armigera increases 20E entering cells [48].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [48]
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- Authors
- Wang, D., Zhao, W.-L., Cai, M.-J., Wang, J.-X., & Zhao, X.-F.
- Affiliations
- Shandong University (CN)
- Corresponding author
- Zhao, Xiao-Fan
- Venue
- Scientific Reports
- Published
- 2015-03-02
- Volume
- 5
- Issue
- 1
- Pages
- 8675-8675
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4345324
- OpenAlex
- W2039504597
- Cited by
- 35
- References
- 40
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2022: 5 · 2021: 5 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 1
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Scientific Reports (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (973 Program, 31230067)
- Ministry of Education of the People's Republic of China (20120131110025)
- National Institutes of Health
- National Institutes of Natural Sciences
- National Key Research and Development Program of China (2012CB114101)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- G protein-coupled receptors, steroid hormone signaling, 20-hydroxyecdysone, Helicoverpa armigera, insect metamorphosis, molting, intracellular calcium, protein phosphorylation, receptor desensitization
- MeSH terms
- Animals, Cell Line, Cell Membrane, Ecdysone, Endocytosis, Hormones, Lepidoptera, Models, Biological, Phosphorylation, Protein Binding, Steroids, Transcription, Genetic, Tritium, Signal Transduction, Up-Regulation, Gene Expression Regulation, Developmental, Molting, Insect Proteins, Gene Silencing, Receptors, G-Protein-Coupled, Gene Knockdown Techniques
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