Intracellular mobilization of Ca2+ by the insect steroid hormone 20-hydroxyecdysone during programmed cell death in silkworm anterior silk glands
Abstract
20-Hydroxyecdysone (20E) triggers programmed cell death (PCD) and regulates de novo gene expression in the anterior silk glands (ASGs) of the silkworm Bombyx mori. PCD is mediated via a nongenomic pathway that includes Ca2+ as a second messenger and the activation of protein kinase C/caspase-3-like protease; however, the steps leading to a concomitant buildup of intracellular Ca2+ are unknown. We employed pharmacological tools to identify the components of this pathway. ASGs were cultured in the presence of 1 microM 20E and one of the following inhibitors: a G-protein-coupled receptor (GPCR) inhibitor, a phospholipase C (PLC) inhibitor, an inositol 1,4,5-trisphosphate receptor (IP3R) antagonist, and an L- or T-type Ca2+ channel blocker. The T-type Ca2+ channel blocker inhibited 20E-induced nuclear and DNA fragmentation; in contrast, PCD was induced by 20E in Ca2+-free medium, indicating that the source of Ca2+ is an intracellular reservoir. The IP3R antagonist inhibited nuclear and DNA fragmentation, suggesting that the endoplasmic reticulum may be the Ca2+ source. Finally, the GPCR and PLC inhibitors effectively blocked nuclear and DNA fragmentation. Our results indicate that 20E increases the intracellular level of Ca2+ by activating IP3R, and that this effect may be brought about by the serial activation of GPCR, PLC, and IP3.
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20E induces a rapid increase of Ca2+ levels in the cells of the anterior silk gland of B. mori via an unknown GPCR pathway.
“Other evidence is provided by the observation that 20E induces rapid increase of Ca2+ levels in the cells of the anterior silk gland of B. mori via an unknown GPCR pathway [40].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [40]
20E increases cytosolic Ca2+ levels by promoting the release of Ca2+ from the intracellular endoplasmic reticulum via an unknown GPCR in silkworm silk glands.
“20E increases the cytosolic Ca2+ levels by promoting the release of Ca2+ from the intracellular endoplasmic reticulum via an unknown GPCR in silkworm silk glands [23].”
From G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane (2014) · cites this paper as [23]
T-type Ca2+ channels are involved in 20E-induced nuclear and DNA fragmentation in silkworm silk glands.
“T-type Ca2+ channels are involved in 20E-induced nuclear and DNA fragmentation in silkworm silk glands [23].”
From G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane (2014) · cites this paper as [23]
20E induces intracellular Ca2+ release into the cytoplasm via an unknown GPCR pathway in the anterior silk gland of silkworms.
“20E induces intracellular Ca2+ release into the cytoplasm via an unknown G-protein-coupled receptor (GPCR) pathway in the anterior silk gland of silkworms [23].”
From G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane (2014) · cites this paper as [23]
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- Authors
- Manaboon, M., Iga, M., Iwami, M., & Sakurai, S.
- Affiliations
- Kanazawa University (JP)
- Corresponding author
- Manaboon, Manaporn
- Venue
- Journal of Insect Physiology
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 55
- Issue
- 2
- Pages
- 123-129
- ISSN
- 0022-1910 (ISSN-L)
- OpenAlex
- W1978047334
- Cited by
- 37
- References
- 48
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 0.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Insect Physiology (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Silkworms and Sericulture Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- 20-hydroxyecdysone, programmed cell death, calcium mobilization, Bombyx mori, IP3 receptor, phospholipase C, G protein-coupled receptors, T-type calcium channels, endoplasmic reticulum calcium stores, non-genomic steroid action, DNA fragmentation
- MeSH terms
- Animals, Calcium, Ecdysterone, Exocrine Glands, Indoles, Bombyx, Second Messenger Systems, Apoptosis, DNA Fragmentation
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