Cited by 4Proteoglycans and glycosaminoglycans researchCholesterol and Lipid MetabolismAlzheimer's disease research and treatments

Role of chondroitin sulphate in the uptake of β‐VLDL by brain cells

Rapp, Alfred, Hüttinger, Manfred·2005·European Journal of Neuroscience

Abstract

Proteoglycans (PGs) have been suggested to work as receptors in lipoprotein uptake mechanisms. An interaction between apolipoprotein E (apoE) and glucosaminoglycans (GAG), polysaccharides linked to proteoglycans, has been proposed in this pathway. At the same time, proteoglycans, apoE as well as lipoprotein receptors have been reported to be constituents of amyloid plaques, one hallmark of Alzheimer's disease. With this study, we are the first to investigate the interaction between beta very low density lipoprotein (β‐VLDL) and a neuronal highly abundant GAG, chondroitin sulphate (CS), comparing hippocampal neurons, expressing high levels of low density lipoprotein receptor related protein (LRP) and U373 astrocytoma cells, highly positive for the low density lipoprotein receptor (LDLR). We were able demonstrate that degradation of chondroitin sulphate proteoglycans (CSPGs) with chondroitinase ABC resulted in reduced 125 I‐β‐VLDL uptake. We showed that externally added CSs compete with internalization of β‐VLDL. The effect was found to be dose‐dependent, but was influenced neither by cell type, nor receptor type. The position of sulphation of added CSs showed only a slight influence. The data generated suggested an interaction between apolipoproteins and soluble CSs; therefore, 3 H‐cholesterol linked to apoE was coadministered with CSs to the cells. The results revealed that apoE bound, but no unbound cholesterol, was reduced in cellular internalization, suggesting that CSPGs may be involved in lipoprotein uptake in the intact brain, mediated, at least in part, by apoE.

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Crossref · 10.1111/j.1460-9568.2005.04313.x

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Authors
Rapp, A., & Hüttinger, M.
Affiliations
Medical University of Vienna (AT)
Corresponding author
Rapp, Alfred
Venue
European Journal of Neuroscience
Publisher
Wiley
Published
2005-09
Volume
22
Issue
6
Pages
1400-1408
ISSN
  • 0953-816X (ISSN-L)
  • 1460-9568
OpenAlex
W1492604886
Cited by
4
References
43
FWCI
0.41
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
chondroitin sulfate, lipoprotein uptake, proteoglycans, chondroitinase ABC, apolipoprotein E, glycosaminoglycans, LDL receptor, LRP, astrocytes, hippocampal neurons, Alzheimer's disease, amyloid plaques, cholesterol, receptor internalization
MeSH terms
Animals, Apolipoproteins E, Astrocytoma, Brain, Brain Chemistry, Cell Line, Cholesterol, Chondroitin Sulfates, Female, Hippocampus, Lipoproteins, VLDL, Neurons, Pregnancy, Spectrometry, Fluorescence, Immunoblotting, Receptors, Lipoprotein, Chondroitin ABC Lyase, Rats

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