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High temporal and inter‐individual variation detected in the human ileal microbiota
Open accessAbstract
Summary: The diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with Brooke ileostomies. The total number of bacteria within the ileal effluent was in the range of 10 7 –10 8 bacteria per gram (wet weight). The diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age‐matched healthy adults. The ileal effluent had a higher relative abundance of species within the orders Lactobacillales and Clostridiales , mainly Streptococcus bovis ‐related species, and the Veillonella group, and a lower proportion of species related to Ruminococcus gnavus , R. obeum and Bacteroides plebeius . In addition, inter‐individual differences were found, indicative of a highly personal ileal microbiota profile. Furthermore, temporal profiles showed large fluctuations per individual over a period of 9–28 days (average similarity over a period of 9 days was as low as 44%), and differences between morning and afternoon profiles were observed. Parallel cloning and sequencing efforts revealed several phylotypes that were not identified in previous studies (12 out of 65 phylotypes showed less than 97% sequence similarity with previously reported sequences). Achaea were found to be below detection limit by quantitative PCR. Overall, the results indicate that the microbiota of the human ileum is relatively unstable, less complex and consisting of different dominating phylotypes when compared with the colonic microbiota.
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Metadata
- Authors
- Booijink, C. C. G. M., El‐Aidy, S., Rajilić‐Stojanović, M., Heilig, H. G. H. J., Troost, F. J., Smidt, H., Kleerebezem, M., De Vos, W. M., & Zoetendal, E. G.
- Affiliations
- TiFN (NL)
- Wageningen University & Research (NL)
- Maastricht University Medical Centre (NL)
- Corresponding author
- Zoetendal, Erwin G.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2010-12
- Volume
- 12
- Issue
- 12
- Pages
- 3213-3227
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2130329894
- Cited by
- 324
- References
- 52
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 17 · 2023: 27 · 2022: 22 · 2021: 23 · 2020: 30 · 2019: 29 · 2018: 24 · 2017: 17 · 2016: 10 · 2015: 15 · 2014: 24 · 2013: 28 · 2012: 18
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Environmental Microbiology (publishedVersion)
- PubMed (publishedVersion)
- TechnoRep (University of Belgrade – Faculty of Technology and Metallurgy) (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Research Publications (Maastricht University) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- ileal microbiota, ileostomy, temporal stability, interindividual variability, bacterial diversity, Lactobacillales, Clostridiales, Streptococcus bovis, Veillonella, Ruminococcus, Bacteroides plebeius, Achaea, novel phylotypes, human gut bacteria
- MeSH terms
- Adult, Aged, Bacteria, Feces, Female, Humans, Ileostomy, Ileum, Male, Middle Aged, Molecular Sequence Data, Phylogeny, Time Factors, Case-Control Studies, Polymerase Chain Reaction, Genes, rRNA, Metagenome
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