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Surface-layer protein extracts from Lactobacillus helveticus inhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithelial cells
Open accessAbstract
Adherence of intestinal pathogens, including Escherichia coli O157:H7, to human intestinal epithelial cells is a key step in pathogenesis. Probiotic bacteria, including Lactobacillus helveticus R0052 inhibit the adhesion of E. coli O157:H7 to epithelial cells, a process which may be related to specific components of the bacterial surface. Surface-layer proteins (Slps) are located in a paracrystalline layer outside the bacterial cell wall and are thought to play a role in tissue adherence. However, the ability of S-layer protein extract derived from probiotic bacteria to block adherence of enteric pathogens has not been investigated. Human epithelial (HEp-2 and T84) cells were treated with S-layer protein extract alone, infected with E. coli O157:H7, or pretreated with S-layer protein extract prior to infection to determine their importance in the inhibition of pathogen adherence. The effects of S-layer protein extracts were characterized by phase-contrast and immunofluorescence microscopy and measurement of the transepithelial electrical resistance of polarized monolayers. Pre-treatment of host epithelial cells with S-layer protein extracts prior to E. coli O157:H7 infection decreased pathogen adherence and attaching-effacing lesions in addition to preserving the barrier function of monolayers. These in vitro studies indicate that a non-viable constituent derived from a probiotic strain may prove effective in interrupting the infectious process of an intestinal pathogen.
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“The S-layer protein extract of L. helveticus R0052 has been shown to ameliorate the pathogenesis of E coli O157:H7 (Sherman et al., 2005; Johnson-Henry et al., 2007).”
From Lactobacillus helveticus: the proteolytic system (2013) · cites this paper as Johnson-Henry et al., 2007
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Metadata
- Authors
- Johnson-Henry, K. C., Hagen, K. E., Gordonpour, M., Tompkins, T. A., & Sherman, P. M.
- Affiliations
- Lallemand (Canada) (CA)
- University of Toronto (CA)
- Corresponding author
- Sherman, Philip M.
- Venue
- Cellular Microbiology
- Publisher
- Hindawi Limited
- Published
- 2007-02
- Volume
- 9
- Issue
- 2
- Pages
- 356-367
- ISSN
- 1462-5814 (ISSN-L)
- 1462-5822
- OpenAlex
- W2151081251
- Cited by
- 274
- References
- 55
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 4 · 2023: 15 · 2022: 19 · 2021: 11 · 2020: 19 · 2019: 14 · 2018: 15 · 2017: 14 · 2016: 15 · 2015: 17 · 2014: 10 · 2013: 17 · 2012: 25
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cellular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Guelph
- Canada Research Chairs
- University of Toronto
- Hospital for Sick Children
- Canadian Institutes of Health Research
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillus helveticus, Escherichia coli O157:H7, surface layer protein, probiotics, bacterial adhesion inhibition, intestinal epithelial cells, attaching and effacing lesions, epithelial barrier function, transepithelial electrical resistance
- MeSH terms
- Bacterial Adhesion, Bacterial Outer Membrane Proteins, Bacterial Proteins, Cell Line, Epithelial Cells, Escherichia coli O157, Lactobacillus helveticus
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