NO APICAL MERISTEM ( MtNAM ) regulates floral organ identity and lateral organ separation in Medicago truncatula
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Summary: The CUP‐SHAPED COTYLEDON ( CUC )/ NO APICAL MERISTEM ( NAM ) family of genes control boundary formation and lateral organ separation, which is critical for proper leaf and flower patterning. However, most downstream targets of CUC/NAM genes remain unclear. In a forward screen of the tobacco retrotransposon1 ( Tnt1 ) insertion population in Medicago truncatula , we isolated a weak allele of the no‐apical‐meristem mutant mtnam‐2 . Meanwhile, we regenerated a mature plant from the null allele mtnam‐1 . These materials allowed us to extensively characterize the function of MtNAM and its downstream genes. MtNAM is highly expressed in vegetative shoot buds and inflorescence apices, specifically at boundaries between the shoot apical meristem and leaf/flower primordia. Mature plants of the regenerated null allele and the weak allele display remarkable floral phenotypes: floral whorls and organ numbers are reduced and the floral organ identity is compromised. Microarray and quantitative RT‐PCR analyses revealed that all classes of floral homeotic genes are down‐regulated in mtnam mutants. Mutations in MtNAM also lead to fused cotyledons and leaflets of the compound leaf as well as a defective shoot apical meristem. Our results revealed that MtNAM shares the role of CUC/NAM family genes in lateral organ separation and compound leaf development, and is also required for floral organ identity and development.
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The NAM/CUC ortholog from Medicago truncatula is necessary for organ separation including compound leaf development, and is also required for floral organ identity and development.
“Recently, it has been reported that the NAM/CUC ortholog from Medicago truncatula is necessary for organ separation including compound leaf development, and is also required for floral organ identity and development (Cheng et al., 2012).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Cheng et al., 2012
In several compound-leaf species, CUC/GOB orthologous genes are expressed in the boundary of leaflets and down-regulation of their expression results in simplification of leaf architecture.
“In several compound-leaf species, CUC/GOB orthologous genes are expressed in the boundary of leaflets and down-regulation of their expression results in simplification of leaf architecture (Blein et al., 2008;Berger et al., 2009;Cheng et al., 2012; Fig. 2d).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Cheng et al., 2012
CUC/GOB genes restrict growth in the boundary zones between initiating leaflets and promote the development of separated units in a complex leaf.
“Similar to their activity at the margin of Arabidopsis leaves, CUC/GOB genes restrict growth in the boundary zones between initiating leaflets and promote, thereby, the development of separated units in a complex leaf.”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Cheng et al., 2012
Cites
Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
Aida, M., Ishida, T., Fukaki, H., Fujisawa, H., & Tasaka, M. (1997) · The Plant Cell · doi:10.1105/tpc.9.6.841 · Cited by 1,486 · see citing works
Souer, E., van Houwelingen, A., Kloos, D., Mol, J., & Koes, R. (1996) · Cell · doi:10.1016/s0092-8674(00)81093-4 · Cited by 1,059 · see citing works
Reddy, G. V., Heisler, M. G., Ehrhardt, D. W., & Meyerowitz, E. M. (2004) · Development · doi:10.1242/dev.01261 · Cited by 339 · see citing works
The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Berger, Y., Harpaz-Saad, S., Brand, A., Melnik, H., Sirding, N., Alvarez, J. P., Zinder, M., Samach, A., Eshed, Y., & Ori, N. (2009) · Development · doi:10.1242/dev.031625 · Cited by 305 · see citing works
Furutani, M., Vernoux, T., Traas, J., Kato, T., Tasaka, M., & Aida, M. (2004) · Development · doi:10.1242/dev.01388 · Cited by 271 · see citing works
<i>CUPULIFORMIS</i> establishes lateral organ boundaries in <i>Antirrhinum</i>
Weir, I., Lu, J., Cook, H., Causier, B., Schwarz-Sommer, Z., & Davies, B. (2004) · Development · doi:10.1242/dev.00993 · Cited by 183 · see citing works
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How boundaries control plant development
Žádníková, P., & Simon, R. (2014) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2013.11.013 · Cited by 121 · see citing works
<i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems
Wang, Q., Hasson, A., Rossmann, S., & Theres, K. (2016) · New Phytologist · doi:10.1111/nph.13641 · Cited by 106 · see citing works
- Authors
- Cheng, X., Peng, J., Ma, J., Tang, Y., Chen, R., Mysore, K. S., & Wen, J.
- Affiliations
- Noble Research Institute (US)
- Corresponding author
- Wen, Jiangqi
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2012-04-24
- Volume
- 195
- Issue
- 1
- Pages
- 71-84
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2029364838
- Cited by
- 93
- References
- 6
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 6 · 2020: 6 · 2019: 10 · 2018: 6 · 2017: 8 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 7 · 2012: 1
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0703285)
- Samuel Roberts Noble Foundation
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- floral organ identity, Medicago truncatula, compound leaf development, shoot apical meristem, floral homeotic genes, boundary formation, floral organ number
- MeSH terms
- Molecular Sequence Data, Mutation, Plant Proteins, Gene Expression Regulation, Plant, Plant Leaves, Meristem, Cotyledon, Retroelements, Flowers, Medicago truncatula, Inflorescence, Genetic Association Studies
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