Regulation of dendritic spine morphology by an NMDA receptor-associated Rho GTPase-activating protein, p250GAP
Abstract
The NMDA receptor regulates spine morphological plasticity by modulating Rho GTPases. However, the molecular mechanisms for NMDA receptor‐mediated regulation of Rho GTPases remain elusive. In this study, we show that p250GAP, an NMDA receptor‐associated RhoGAP, regulates spine morphogenesis by modulating RhoA activity. Knock‐down of p250GAP increased spine width and elevated the endogenous RhoA activity in primary hippocampal neurons. The increased spine width by p250GAP knock‐down was suppressed by the expression of a dominant‐negative form of RhoA. Furthermore, p250GAP is involved in NMDA receptor‐mediated RhoA activation. In response to NMDA receptor activation, exogenously expressed green fluorescent protein (GFP)‐tagged p250GAP was redistributed. Thus, these data suggest that p250GAP plays an important role in NMDA receptor‐mediated regulation of RhoA activity leading to spine morphological plasticity.
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RNAi-mediated knockdown of the known miR-132 target p250GAP increases dendritic spine size.
“RNAi-mediated knockdown of the known miR-132 target p250GAP was recently found to increase dendritic spine size (Nakazawa et al., 2008; Vo et al., 2005; Wayman et al., 2008).”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Nakazawa et al., 2008
Knockdown of p250GAP enlarges dendritic spines in mouse neurons.
“Knockdown of p250GAP has recently been shown to enlarge dendritic spines in mouse neurons (Nakazawa et al., 2008)”
From Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132 (2010) · cites this paper as Nakazawa et al., 2008
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Cites
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Regulation of Synaptic Structure and Function by FMRP-Associated MicroRNAs miR-125b and miR-132
Edbauer, D., Neilson, J. R., Foster, K. A., Wang, C.-F., Seeburg, D. P., Batterton, M. N., Tada, T., Dolan, B. M., Sharp, P. A., & Sheng, M. (2010) · Neuron · doi:10.1016/j.neuron.2010.01.005 · Cited by 735 · see citing works
Dendritic spine actin cytoskeleton in autism spectrum disorder
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Warnet, X. L., Bakke Krog, H., Sevillano‐Quispe, O. G., Poulsen, H., & Kjaergaard, M. (2021) · European Journal of Neuroscience · doi:10.1111/ejn.14842 · Cited by 50 · see citing works
- Authors
- Nakazawa, T., Kuriu, T., Tezuka, T., Umemori, H., Okabe, S., & Yamamoto, T.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 2008-03-10
- Volume
- 105
- Issue
- 4
- Pages
- 1384-1393
- ISSN
- 0022-3042
- 1471-4159
- Cited by
- 59
- References
- 8
- Language
- en
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