The DOI matches its Crossref record; 72 of the 72 references it cites were found in a registry or an index.
Mitochondrial functional impairment with aging is exaggerated in isolated mitochondria compared to permeabilized myofibers
Open accessAbstract
Summary: Mitochondria regulate cellular bioenergetics and apoptosis and have been implicated in aging. However, it remains unclear whether age‐related loss of muscle mass, known as sarcopenia, is associated with abnormal mitochondrial function. Two technically different approaches have mainly been used to measure mitochondrial function: isolated mitochondria and permeabilized myofiber bundles, but the reliability of these measures in the context of sarcopenia has not been systematically assessed before. A key difference between these approaches is that contrary to isolated mitochondria, permeabilized bundles contain the totality of fiber mitochondria where normal mitochondrial morphology and intracellular interactions are preserved. Using the gastrocnemius muscle from young adult and senescent rats, we show marked effects of aging on three primary indices of mitochondrial function (respiration, H 2 O 2 emission, sensitivity of permeability transition pore to Ca 2+ ) when measured in isolated mitochondria, but to a much lesser degree when measured in permeabilized bundles. Our results clearly demonstrate that mitochondrial isolation procedures typically employed to study aged muscles expose functional impairments not seen in situ . We conclude that aging is associated with more modest changes in mitochondrial function in sarcopenic muscle than suggested previously from isolated organelle studies.
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“Furthermore, several of these investigations were performed in isolated mitochondria (6,9,11), which has been shown to exaggerate the observed deficit in mitochondrial function, when compared to measurements conducted on permeabilized myofibers (17).”
From Chronological Age Does not Influence Ex-vivo Mitochondrial Respiration and Quality Control in Skeletal Muscle (2016) · cites this paper as [17]
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Cites
1 retracted
Wenz, T., Rossi, S. G., Rotundo, R. L., Spiegelman, B. M., & Moraes, C. T. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911570106 · Cited by 600
RetractedThe mitochondrial permeability transition
Zoratti, M., & Szabó, I. (1995) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(95)00003-a · Cited by 2,410
St-Pierre, J., Drori, S., Uldry, M., Silvaggi, J. M., Rhee, J., Jäger, S., Handschin, C., Zheng, K., Lin, J., Yang, W., et al. (2006) · Cell · doi:10.1016/j.cell.2006.09.024 · Cited by 2,233
Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression
Lecker, S. H., Jagoe, R. T., Gilbert, A., Gomes, M., Baracos, V., Bailey, J., Price, S. R., Mitch, W. E., & Goldberg, A. L. (2004) · The FASEB Journal · doi:10.1096/fj.03-0610com · Cited by 1,539 · cited 1×
“Likewise, several metabolic changes occur with muscle atrophy (Lecker et al., 2004), and recent evidence suggests that mitochondrial function can be a key regulator of the atrophic process (Romanello et al., 2010).”
BackgroundFunctions and dysfunctions of mitochondrial dynamics
Detmer, S. A., & Chan, D. C. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2275 · Cited by 1,369
Decline in skeletal muscle mitochondrial function with aging in humans
Short, K. R., Bigelow, M. L., Kahl, J., Singh, R., Coenen-Schimke, J., Raghavakaimal, S., & Nair, K. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501559102 · Cited by 1,333
Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts
Frezza, C., Cipolat, S., & Scorrano, L. (2007) · Nature Protocols · doi:10.1038/nprot.2006.478 · Cited by 1,188
Muscle fiber type composition of the rat hindlimb
Armstrong, R. B., & Phelps, R. O. (1984) · American Journal of Anatomy · doi:10.1002/aja.1001710303 · Cited by 1,004
Inhibiting Mitochondrial Fission Protects the Heart Against Ischemia/Reperfusion Injury
Ong, S.-B., Subrayan, S., Lim, S. Y., Yellon, D. M., Davidson, S. M., & Hausenloy, D. J. (2010) · Circulation · doi:10.1161/circulationaha.109.906610 · Cited by 986
Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells
Kuznetsov, A. V., Veksler, V., Gellerich, F. N., Saks, V. A., Margreiter, R., & Kunz, W. S. (2008) · Nature Protocols · doi:10.1038/nprot.2008.61 · Cited by 839
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Sorted by citations
Sarcopenia: Aging-Related Loss of Muscle Mass and Function
Larsson, L., Degens, H., Li, M., Salviati, L., Lee, Y. I., Thompson, W., Kirkland, J. L., & Sandri, M. (2019) · Physiological Reviews · doi:10.1152/physrev.00061.2017 · Cited by 1,797
The role of mitochondria in aging
Bratic, A., & Larsson, N.-G. (2013) · Journal of Clinical Investigation · doi:10.1172/jci64125 · Cited by 1,087
Effects of Exercise and Aging on Skeletal MusclePreprint
Distéfano, G., & Goodpaster, B. H. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a029785 · Cited by 530
Exercise Promotes Healthy Aging of Skeletal Muscle
Cartee, G. D., Hepple, R. T., Bamman, M. M., & Zierath, J. R. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.05.007 · Cited by 483
Self-citationPGC-1α-mediated regulation of mitochondrial function and physiological implications
Halling, J. F., & Pilegaard, H. (2020) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/apnm-2020-0005 · Cited by 430
Metadata
- Authors
- Picard, M., Ritchie, D., Wright, K. J., Romestaing, C., Thomas, M. M., Rowan, S. L., Taivassalo, T., & Hepple, R. T.
- Affiliations
- McGill University (CA)
- University of Calgary (CA)
- Université de Lyon (FR)
- Corresponding author
- Hepple, Russell T.
- Venue
- Aging Cell
- Publisher
- Wiley
- Published
- 2010-11-10
- Volume
- 9
- Issue
- 6
- Pages
- 1032-1046
- ISSN
- 1474-9718 (ISSN-L)
- 1474-9726
- OpenAlex
- W1600619258
- Cited by
- 238
- References
- 72
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 20 · 2023: 13 · 2022: 12 · 2021: 17 · 2020: 12 · 2019: 12 · 2018: 25 · 2017: 19 · 2016: 17 · 2015: 9 · 2014: 10 · 2013: 25 · 2012: 16
- FWCI
- 7.15
- Type
- journal article
- Language
- en
- Open access
- Locations
- Aging Cell (publishedVersion)
- PubMed (publishedVersion)
- eScholarship@McGill (McGill) (submittedVersion)
- Funders
- University of Calgary
- Directorate for Biological Sciences
- Canadian Institutes of Health Research (84673_1, MOP57808)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- mitochondrial function, aging, sarcopenia, isolated mitochondria, mitochondrial respiration, hydrogen peroxide emission, mitochondrial permeability transition pore, calcium sensitivity, gastrocnemius
- MeSH terms
- Aging, Animals, Calcium, Hydrogen Peroxide, Male, Mitochondria, Mitochondria, Muscle, Permeability, Rats, Inbred F344, Rats, Inbred Strains, Reactive Oxygen Species, Mitochondrial Membrane Transport Proteins, Rats
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