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Cited by 102Mitochondrial Function and PathologyMuscle Physiology and DisordersAdipose Tissue and Metabolism

Aging impairs skeletal muscle mitochondrial bioenergetic function

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Figueiredo, P. A., Powers, S. K., Ferreira, R. M., Appell, H. J., Duarte, J. A.·2009·The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
DOI 10.1093/gerona/gln048PMC PMC2691197
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Abstract

This study investigated the influence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O(2) consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde) were measured in isolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confirm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is significantly correlated with higher levels of mitochondrial oxidative damage.

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Crossref · 10.1093/gerona/gln048

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  • There is substantial evidence of increased ROS production and oxidative stress-induced damage to mtDNA and ETC complexes in aged rodents and non-human primates, as well as in human vastus lateralis muscle.

    “there is substantial evidence showing increased ROS production and associated oxidative stress-induced damage to mitochondrial DNA (mtDNA) and ETC complexes in aged rodents and non-human primates (Figueiredo, Powers, Ferreira, Appell, and Duarte 2009;Lee, Choi, Birkenfeld, Alves, Jornayvaz, Jurczak, Zhang, Woo, Shadel, and Ladiges 2010;Lee, Wang, and Fanburg 1998;WANAGAT, CAO, PATHARE, and AIKEN 2”

    From Successful aging: Advancing the science of physical independence in older adults (2015) · cites this paper as Figueiredo et al. 2009

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Authors
Figueiredo, P. A., Powers, S. K., Ferreira, R. M., Appell, H. J., & Duarte, J. A.
Affiliations
  • Universidade do Porto (PT)
  • University of Florida (US)
  • German Sport University Cologne (DE)
ORCID
Venue
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Publisher
Oxford University Press (OUP)
Published
2009-01-01
Volume
64A
Issue
1
Pages
21-33
ISSN
  • 1079-5006 (ISSN-L)
  • 1758-535X
DOI
10.1093/gerona/gln048
PMC
PMC2691197
OpenAlex
W2144365152
Cited by
102
References
60
Citations by year
2026: 3 · 2025: 5 · 2024: 3 · 2023: 7 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 8 · 2014: 7 · 2013: 10 · 2012: 7
FWCI
4.02
Type
journal article
Language
en
Open access
Locations
  • The Journals of Gerontology Series A (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
aging, skeletal muscle, mitochondrial function, mitochondrial bioenergetics, oxidative damage, respiratory control ratio, ADP phosphorylation, aconitase activity, protein carbonylation, malondialdehyde, sulfhydryl groups, mitochondrial homeostasis, C57BL/6 mice
MeSH terms
Aging, Animals, Citrate (si)-Synthase, Electrophoresis, Polyacrylamide Gel, Energy Metabolism, Male, Mice, Inbred C57BL, Mitochondria, Muscle, Muscle Proteins, Lipid Peroxidation, Oxidative Stress, Muscle, Skeletal, Cytochromes c, Microscopy, Electron, Transmission, Mice

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