INTERCOLONIAL VARIABILITY IN MACROMOLECULAR COMPOSITION IN P‐STARVED AND P‐REPLETE SCENEDESMUS POPULATIONS REVEALED BY INFRARED MICROSPECTROSCOPY1
Abstract
Macromolecular variability in microalgal populations subject to different nutrient environments was investigated, using the chlorophyte alga Scenedesmus quadricauda (Turpin) Bréb. as a model organism. The large size of the four-cell coenobia in the strain used in this study (∼35 μm diameter) conveniently allowed high quality spectra to be obtained from individual coenobia using a laboratory-based Fourier transform infrared (FTIR) microscope with a conventional globar source of IR. By drawing sizable subpopulations of coenobia from two Scenedesmus cultures grown under either nutrient-replete or P-starved conditions, the population variability in macromolecular composition, and the effects of nutrient change upon this, could be estimated. On average, P-starved coenobia had higher carbohydrate and lower protein absorbance compared with P-replete coenobia. These parameters varied between coenobia with histograms of the ratio of absorbance of the largest protein and carbohydrate bands being Gaussian distributed. Distributions for the P-replete and P-starved subpopulations were nonoverlapping, with the difference in mean ratios for the two populations being statistically significant. Greater variance was observed in the P-starved subpopulation. In addition, multivariate models were developed using the spectral data, which could accurately predict the nutrient status of an independent individual coenobium, based on its FTIR spectrum. Partial least squares discriminant analysis (PLS-DA) was a better prediction method compared with soft independent modeling by class analogy (SIMCA).
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FTIR with multivariate analysis has been used successfully to classify species and to differentiate the nutritional status of microalgae.
“The application of FTIR to classify species with the help of multivariate analysis [24 -29] and to differentiate the nutritional status of microalgae [30 -34] have been attempted successfully.”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [32]
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FT-IR Microspectroscopy Enhances Biological and Ecological Analysis of Algae
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- Authors
- Heraud, P., Stojkovic, S., Beardall, J., McNaughton, D., & Wood, B. R.
- Affiliations
- Monash University (AU)
- Corresponding author
- Heraud, Philip
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 2008-10
- Volume
- 44
- Issue
- 5
- Pages
- 1335-1339
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W1986105905
- Cited by
- 32
- References
- 17
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Phycology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- Scenedesmus quadricauda, phosphorus deficiency, macromolecular composition, intraspecific variation, coenobia, carbohydrate content, protein content, FTIR microspectroscopy, PLS-DA, SIMCA
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