The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Human platelet lysate can replace fetal bovine serum for clinical‐scale expansion of functional mesenchymal stromal cells
Abstract
BACKGROUND: Human multipotent mesenchymal stromal cells (MSCs) are promising candidates for a growing spectrum of regenerative and immunomodulatory cellular therapies. Translation of auspicious experimental results into clinical applications has been limited by the dependence of MSC propagation from fetal bovine serum (FBS). STUDY DESIGN AND METHODS: The capacity of human platelet lysate (HPL) to replace FBS for clinical‐scale MSC propagation was analyzed. RESULTS: HPL could be efficiently produced from buffy coats. Multiplex analyses allowed a distinct HPL growth factor profile to be delineated. With a previously established two‐step clinical‐scale procedure, HPL was reproducibly more efficient than FBS in supporting MSC outgrowth. With only 3 × 10 5 primary culture‐derived MSCs, a mean of 4.36 × 10 8 HPL‐MSCs (range, 3.01 × 10 8 ‐5.40 × 10 8 ) was obtained within a single secondary 11‐ to 13‐day culture step. Although morphologically distinct, HPL‐MSCs and FBS‐MSCs did not differ significantly in terms of immunophenotype, differentiation potential in vitro, and lack of tumorigenicity in nude mice in vivo. CONCLUSIONS: Replacing FBS with HPL prevents bovine prion, viral, and zoonose contamination of the stem cell product. This new efficient FBS‐free two‐step procedure for clinical‐scale MSC propagation may represent a major step toward challenging new stem cell therapies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Le Blanc, K., Rasmusson, I., Sundberg, B., Götherström, C., Hassan, M., Uzunel, M., & Ringdén, O. (2004) · The Lancet · doi:10.1016/s0140-6736(04)16104-7 · Cited by 2,734
Marx, R. E., Carlson, E. R., Eichstaedt, R. M., Schimmele, S. R., Strauss, J. E., & Georgeff, K. R. (1998) · Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology · doi:10.1016/s1079-2104(98)90029-4 · Cited by 2,598
Horwitz, E. M., Gordon, P. L., Koo, W. K. K., Marx, J. C., Neel, M. D., McNall, R. Y., Muul, L., & Hofmann, T. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.132252399 · Cited by 1,650
Autologous platelets as a source of proteins for healing and tissue regeneration
Anitua, E., Andia, I., Ardanza, B., Nurden, P., & Nurden, A. (2004) · Thrombosis and Haemostasis · doi:10.1160/th03-07-0440 · Cited by 1,495
Mesenchymal Stem Cells for Treatment of Therapy-Resistant Graft-versus-Host Disease
Ringdén, O., Uzunel, M., Rasmusson, I., Remberger, M., Sundberg, B., Lönnies, H., Marschall, H.-U., Dlugosz, A., Szakos, A., Hassan, Z., et al. (2006) · Transplantation · doi:10.1097/01.tp.0000214462.63943.14 · Cited by 1,142
Koç, O. N., Gerson, S. L., Cooper, B. W., Dyhouse, S. M., Haynesworth, S. E., Caplan, A. I., & Lazarus, H. M. (2000) · Journal of Clinical Oncology · doi:10.1200/jco.2000.18.2.307 · Cited by 1,119
Friedenstein, A. J., Uf, D., Nn, K., Af, P., Sf, R., Ea, L., & Ia, R. (1974) · PubMed · Cited by 1,015
Lazarus, H. M., Koc, O. N., Devine, S. M., Curtin, P., Maziarz, R. T., Holland, H. K., Shpall, E. J., McCarthy, P., Atkinson, K., Cooper, B. W., et al. (2005) · Biology of Blood and Marrow Transplantation · doi:10.1016/j.bbmt.2005.02.001 · Cited by 803
Lazarus, H. M., Haynesworth, S. E., Gerson, S. L., Rosenthal, N. S., & Caplan, A. I. (1995) · PubMed · Cited by 749
Doucet, C., Ernou, I., Zhang, Y., Llense, J.-R., Begot, L., Holy, X., & Lataillade, J.-J. (2005) · Journal of Cellular Physiology · doi:10.1002/jcp.20391 · Cited by 689
Cited by
Advancing biomaterials of human origin for tissue engineering
Chen, F.-M., & Liu, X. (2016) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2015.02.004 · Cited by 1,158
Fetal Bovine Serum (FBS): past ‐ present ‐ future
van der Valk, J. (2018) · ALTEX · doi:10.14573/altex.1705101 · Cited by 481
Li, C.-Y., Wu, X.-Y., Tong, J.-B., Yang, X.-X., Zhao, J.-L., Zheng, Q.-F., Zhao, G.-B., & Ma, Z.-J. (2015) · Stem Cell Research & Therapy · doi:10.1186/s13287-015-0066-5 · Cited by 410
J. Centeno, C., R. Schultz, J., Cheever, M., Freeman, M., Faulkner, S., Robinson, B., & Hanson, R. (2011) · Current Stem Cell Research & Therapy · doi:10.2174/157488811797904371 · Cited by 234
Blázquez-Prunera, A., Díez, J. M., Gajardo, R., & Grancha, S. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0552-z · Cited by 79
Metadata
- Authors
- Schallmoser, K., Bartmann, C., Rohde, E., Reinisch, A., Kashofer, K., Stadelmeyer, E., Drexler, C., Lanzer, G., Linkesch, W., & Strunk, D.
- Venue
- Transfusion
- Publisher
- Wiley
- Published
- 2007-05-03
- Volume
- 47
- Issue
- 8
- Pages
- 1436-1446
- ISSN
- 0041-1132 (ISSN-L)
- 1537-2995
- OpenAlex
- W1984180661
- Cited by
- 503
- References
- 45
- FWCI
- 10.72
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Human platelet lysate can replace fetal bovine serum for clinical‐scale expansion of functional mesenchymal stromal cells. https://app.cochat.ai/papers/10.1111/j.1537-2995.2007.01220.x
- The paper
- Schallmoser, K., Bartmann, C., Rohde, E., Reinisch, A., Kashofer, K., Stadelmeyer, E., Drexler, C., Lanzer, G., Linkesch, W., & Strunk, D. (2007). Human platelet lysate can replace fetal bovine serum for clinical‐scale expansion of functional mesenchymal stromal cells. Transfusion. https://doi.org/10.1111/j.1537-2995.2007.01220.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
