The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata: Transcriptional regulation of YPS1 in C. glabrata
Open accessAbstract
In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is not well understood. Here, we report that C. glabrata Yps1 is required for cell growth at elevated temperatures, and that the heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Although a total of 11 YPS genes are present in the C. glabrata genome, the loss of transcriptional induction in a calcineurin mutant was observed only for YPS1. The results of a YPS1 promoter-lacZ reporter assay using a series of constructs with mutated promoter elements indicated that the transcription factor Crz1 binds to multiple sites in the promoter region of YPS1. To date, as none of the putative Crz1 targets in C. glabrata have been characterized using a Δcrz1 mutant, monitoring the expression of YPS1 represents an effective method for measuring the activity of the calcineurin-Crz1 signaling pathway in this fungus.
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CgYPS genes are regulated by various environmental conditions including low pH, thermal stress, macrophage and neutrophil internalization.
“CgYPS genes have been shown to be regulated by various environmental conditions including low pH, thermal stress, macrophage and neutrophil internalization [60,61,62,63].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [62]
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- Authors
- Miyazaki, T., Izumikawa, K., Yamauchi, S., Inamine, T., Nagayoshi, Y., Saijo, T., Seki, M., Kakeya, H., Yamamoto, Y., Yanagihara, K., Miyazaki, Y., Yasuoka, A., & Kohno, S.
- Affiliations
- Nagasaki University (JP)
- National Institute of Infectious Diseases (JP)
- Nagasaki University Hospital (JP)
- Corresponding author
- Miyazaki, Taiga
- ORCID
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-05-16
- Volume
- 11
- Issue
- 5
- Pages
- 449-456
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W1961697585
- Cited by
- 23
- References
- 20
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 1 · 2022: 3 · 2020: 2 · 2019: 2 · 2017: 2 · 2016: 1 · 2014: 1 · 2013: 4 · 2012: 1
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (acceptedVersion)
- Nagasaki University's Academic Output SITE (Nagasaki University) (submittedVersion, cc-by-nc-nd)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (21390305, 20591212)
- Ministry of Health, Labour and Welfare
- Japan Society for the Promotion of Science (19790324)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida glabrata, transcriptional regulation, heat stress response, cell wall integrity, virulence, promoter analysis
- MeSH terms
- beta-Galactosidase, Candidiasis, Cell Wall, Fungal Proteins, Genetic Complementation Test, Hot Temperature, Mutation, Promoter Regions, Genetic, RNA, Fungal, RNA, Messenger, Transcription Factors, Virulence, Signal Transduction, Gene Expression Regulation, Fungal, Glycosylphosphatidylinositols, Calcineurin, Candida glabrata, Aspartic Acid Proteases
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