Functional characterization of the regulators of calcineurin in Candida glabrata
Open accessAbstract
The serine-threonine-specific protein phosphatase calcineurin is a key mediator of various stress responses in fungi. Herein, we characterized functions of the endogenous regulators of calcineurin (RCNs), Rcn1 and Rcn2, in the pathogenic fungus Candida glabrata. Rcn1 exerted both inhibitory and stimulatory effects on calcineurin signaling, but Rcn2 displayed only inhibitory activity. Phenotypic analyses of C. glabrata strains lacking either RCNs, calcineurin, or both revealed that calcineurin requires Rcn1, but not Rcn2, for antifungal tolerance in C. glabrata.
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Rcn2 functions in its canonical role as a feedback inhibitor of calcineurin signaling, rather than as an effector of calcineurin signaling in the regulation of tolerance and persistence.
“These findings suggest that Rcn2 functions in its canonical role as a feedback inhibitor of calcineurin signaling (28, 37), rather than as an effector of calcineurin signaling in the regulation of tolerance and persistence.”
From Echinocandin tolerance and persistence <i>in vitro</i> are regulated by calcineurin signaling in <i>Candida glabrata</i> (2026) · cites this paper as [37]
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“These findings suggest that Rcn2 functions in its canonical role as a feedback inhibitor of calcineurin signaling (28, 37), rather than as an effector of calcineurin signaling in the regulation of tolerance and persistence.”
- Authors
- Miyazaki, T., Izumikawa, K., Nagayoshi, Y., Saijo, T., Yamauchi, S., Morinaga, Y., Seki, M., Kakeya, H., Yamamoto, Y., Yanagihara, K., Miyazaki, Y., & Kohno, S.
- Affiliations
- Nagasaki University (JP)
- National Institute of Infectious Diseases (JP)
- Corresponding author
- Miyazaki, Taiga
- ORCID
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-09-19
- Volume
- 11
- Issue
- 8
- Pages
- 621-630
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W2132691039
- Cited by
- 17
- References
- 23
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 2 · 2021: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Nagasaki University's Academic Output SITE (Nagasaki University) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- calcineurin, RCN1, Candida glabrata, protein phosphatase, stress response, antifungal tolerance, calcineurin signaling, fungal pathogenesis
- MeSH terms
- Micafungin, Amino Acid Sequence, Anti-Bacterial Agents, Antifungal Agents, Fungal Proteins, Molecular Sequence Data, RNA, Fungal, RNA, Messenger, Saccharomyces cerevisiae, Stress, Physiological, Tunicamycin, Signal Transduction, Fluconazole, Sequence Alignment, Sequence Deletion, Calcineurin, Drug Resistance, Fungal, Candida glabrata, Intracellular Signaling Peptides and Proteins, Echinocandins, Lipopeptides, Calcineurin Inhibitors
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