Skn7p is involved in oxidative stress response and virulence of Candida glabrata
Abstract
Candida glabrata is an opportunistic fungal pathogen that causes both superficial and deep-seated mycosis in humans. In Saccharomyces cerevisiae and several pathogenic fungi, Skn7p is known as a transcriptional factor involved in oxidative stress response (OSR) but functions of its ortholog have been little investigated in C. glabrata. In this study, we constructed a C. glabrata skn7 deletion strain by the ura-blaster technique and investigated mutant phenotypes related to OSR and virulence. The C. glabrata skn7 deletant showed increased susceptibility to hydrogen peroxide and tert-butyl hydroperoxide. Our transcriptional assay evaluated by quantitative real-time PCR revealed that, in response to the treatment with hydrogen peroxide, transcription of some putative Skn7p target genes including TRX2, TRR1, TSA1 and CTA1 were not fully induced in the skn7 deletant compared to the wild-type control, consistent with the susceptibility phenotype. Furthermore, the deletion of SKN7 resulted in attenuated virulence in a murine model of disseminated candidiasis. These results suggest that Skn7p may play a role in transcriptional regulation of its target genes required for OSR and virulence in C. glabrata.
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CgCTA1 expression is regulated by several stress response transcriptional regulators including CgYap1, CgMsn2, CgMsn4 and CgSkn7.
“CgCTA1 expression is regulated by several stress response transcriptional regulators including CgYap1, CgMsn2, CgMsn4 and CgSkn7 [80,84,85,86].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [86]
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- Authors
- Saijo, T., Miyazaki, T., Izumikawa, K., Mihara, T., Takazono, T., Kosai, K., Imamura, Y., Seki, M., Kakeya, H., Yamamoto, Y., Yanagihara, K., & Kohno, S.
- Affiliations
- Nagasaki University (JP)
- Corresponding author
- Izumikawa, Koichi
- ORCID
- Venue
- Mycopathologia
- Published
- 2009-08-20
- Volume
- 169
- Issue
- 2
- Pages
- 81-90
- ISSN
- 0301-486X (ISSN-L)
- 1573-0832
- OpenAlex
- W1968299410
- Cited by
- 59
- References
- 41
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 6 · 2012: 3
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Mycopathologia (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- Candida glabrata, oxidative stress response, virulence, transcriptional regulation, hydrogen peroxide susceptibility, tert-butyl hydroperoxide, Trx2, Tsa1, systemic candidiasis, opportunistic fungal pathogens
- MeSH terms
- Animals, Candidiasis, Disease Models, Animal, DNA-Binding Proteins, Female, Fungal Proteins, Humans, Hydrogen Peroxide, Kidney, Liver, Mice, Inbred C57BL, Spleen, Transcription Factors, Colony Count, Microbial, Oxidants, Gene Deletion, Oxidative Stress, tert-Butylhydroperoxide, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Candida glabrata, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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