Cited by 59Fungal and yeast genetics researchAntifungal resistance and susceptibilityRedox biology and oxidative stress

Skn7p is involved in oxidative stress response and virulence of Candida glabrata

Saijo, Tomomi, Miyazaki, Taiga, Izumikawa, Koichi, Mihara, Tomo, Takazono, Takahiro, Kosai, Kosuke, Imamura, Yoshifumi, Seki, Masafumi +4 more·2010·Mycopathologia

Abstract

Candida glabrata is an opportunistic fungal pathogen that causes both superficial and deep-seated mycosis in humans. In Saccharomyces cerevisiae and several pathogenic fungi, Skn7p is known as a transcriptional factor involved in oxidative stress response (OSR) but functions of its ortholog have been little investigated in C. glabrata. In this study, we constructed a C. glabrata skn7 deletion strain by the ura-blaster technique and investigated mutant phenotypes related to OSR and virulence. The C. glabrata skn7 deletant showed increased susceptibility to hydrogen peroxide and tert-butyl hydroperoxide. Our transcriptional assay evaluated by quantitative real-time PCR revealed that, in response to the treatment with hydrogen peroxide, transcription of some putative Skn7p target genes including TRX2, TRR1, TSA1 and CTA1 were not fully induced in the skn7 deletant compared to the wild-type control, consistent with the susceptibility phenotype. Furthermore, the deletion of SKN7 resulted in attenuated virulence in a murine model of disseminated candidiasis. These results suggest that Skn7p may play a role in transcriptional regulation of its target genes required for OSR and virulence in C. glabrata.

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Crossref · 10.1007/s11046-009-9233-5

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  • CgCTA1 expression is regulated by several stress response transcriptional regulators including CgYap1, CgMsn2, CgMsn4 and CgSkn7.

    “CgCTA1 expression is regulated by several stress response transcriptional regulators including CgYap1, CgMsn2, CgMsn4 and CgSkn7 [80,84,85,86].”

    From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [86]

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Authors
Saijo, T., Miyazaki, T., Izumikawa, K., Mihara, T., Takazono, T., Kosai, K., Imamura, Y., Seki, M., Kakeya, H., Yamamoto, Y., Yanagihara, K., & Kohno, S.
Affiliations
Nagasaki University (JP)
Corresponding author
Izumikawa, Koichi
Venue
Mycopathologia
Published
2009-08-20
Volume
169
Issue
2
Pages
81-90
ISSN
  • 0301-486X (ISSN-L)
  • 1573-0832
OpenAlex
W1968299410
Cited by
59
References
41
Citations by year
2026: 2 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 6 · 2012: 3
FWCI
1.74
Type
journal article
Language
en
Open access
closed
Locations
  • Mycopathologia (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
Candida glabrata, oxidative stress response, virulence, transcriptional regulation, hydrogen peroxide susceptibility, tert-butyl hydroperoxide, Trx2, Tsa1, systemic candidiasis, opportunistic fungal pathogens
MeSH terms
Animals, Candidiasis, Disease Models, Animal, DNA-Binding Proteins, Female, Fungal Proteins, Humans, Hydrogen Peroxide, Kidney, Liver, Mice, Inbred C57BL, Spleen, Transcription Factors, Colony Count, Microbial, Oxidants, Gene Deletion, Oxidative Stress, tert-Butylhydroperoxide, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Candida glabrata, Mice

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