Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2.
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We aimed to manipulate the metabolism of Saccharomyces cerevisiae to produce lactic acid and search for the potential influence of acid transport across the plasma membrane in this process. Saccharomyces cerevisiae W303-1A is able to use l-lactic acid but its production in our laboratory has not previously been detected. When the l-LDH gene from Lactobacillus casei was expressed in S. cerevisiae W303-1A and in the isogenic mutants jen1∆, ady2∆ and jen1∆ ady2∆, all strains were able to produce lactic acid, but higher titres were achieved in the mutant strains. In strains constitutively expressing both LDH and JEN1 or ADY2, a higher external lactic acid concentration was found when glucose was present in the medium, but when glucose was exhausted, its consumption was more pronounced. These results demonstrate that expression of monocarboxylate permeases influences lactic acid production. Ady2 has been previously characterized as an acetate permease but our results demonstrated its additional role in lactate uptake. Overall, we demonstrate that monocarboxylate transporters Jen1 and Ady2 are modulators of lactic acid production and may well be used to manipulate lactic acid export in yeast cells.
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In S. cerevisiae, ADY2 (also called ATO1) encodes a permease-mediating uptake of acetate-propionate-formate present in ethanol or acetate-grown cells.
“In S. cerevisiae, ADY2 (also called ATO1) encodes a permease-mediating uptake of acetate–propionate–formate present in ethanol or acetate-grown cells (Casal et al., 1996; Paiva et al., 2004; Pacheco et al., 2012).”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Pacheco et al., 2012
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- Authors
- Pacheco, A., Talaia, G., Sá-Pessoa, J., Bessa, D., Gonçalves, M. J., Moreira, R., Paiva, S., Casal, M., & Queirós, O.
- Affiliations
- Cooperativa de Ensino Superior Politécnico e Universitário (PT)
- University of Minho (PT)
- CBMA - Center of Molecular and Environmental Biology; Department of Biology; University of Minho; Braga; Portugal
- Corresponding author
- Queirós, Odília
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-02-13
- Volume
- 12
- Issue
- 3
- Pages
- 375-381
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W1968652158
- Cited by
- 104
- References
- 25
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 7 · 2023: 13 · 2022: 9 · 2021: 8 · 2020: 6 · 2019: 10 · 2018: 6 · 2017: 6 · 2016: 3 · 2015: 12 · 2014: 5 · 2013: 6
- FWCI
- 2.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cooperativa de Ensino Superior Politécnico e Universitário
- Fundação para a Ciência e a Tecnologia (COMPETE, PEst-C/BIA/UI4050/2011)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- lactic acid production, Saccharomyces cerevisiae, monocarboxylate transporters, lactate utilization, lactate dehydrogenase, plasma membrane transport, glucose utilization
- MeSH terms
- Cell Membrane, Genetic Engineering, L-Lactate Dehydrogenase, Lacticaseibacillus casei, Mutation, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Lactic Acid, Membrane Transport Proteins, Monocarboxylic Acid Transporters, Symporters, Saccharomyces cerevisiae Proteins
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